Flow cytometric determination of the effects of antibacterial agents on Mycoplasma agalactiae, Mycoplasma putrefaciens, Mycoplasma capricolum subsp capricolum, and Mycoplasma mycoides subsp mycoides large colony type

Patrícia Assunção; Nuno T. Antunes; Ruben S. Rosales; Carlos Poveda; José B. Poveda; Hazel M. Davey

doi:10.1128/AAC.01582-05

Flow cytometric determination of the effects of antibacterial agents on Mycoplasma agalactiae, Mycoplasma putrefaciens, Mycoplasma capricolum subsp capricolum, and Mycoplasma mycoides subsp mycoides large colony type

Patrícia Assunção
, Nuno T. Antunes
, Ruben S. Rosales
, Carlos Poveda
, José B. Poveda
, Hazel M. Davey

Institute of Biological, Environmental, and Rural Sciences

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Flow cytometry together with SYBR green I and propidium iodide was used to study the effects of enrofloxacin, ciprofloxacin, gentamicin, chloramphenicol, oxytetracycline, and tylosin on four mycoplasma species. Inhibition of mycoplasma growth could be detected by as early as 3 h after the start of treatment. The strongest effect was observed with enrofloxacin- and ciprofloxacin-treated cells.

Original language	English
Pages (from-to)	2845-2849
Number of pages	5
Journal	Antimicrobial Agents and Chemotherapy
Volume	50
Issue number	8
DOIs	https://doi.org/10.1128/AAC.01582-05
Publication status	Published - 13 May 2006

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Assunção, P., Antunes, N. T., Rosales, R. S., Poveda, C., Poveda, J. B., & Davey, H. M. (2006). Flow cytometric determination of the effects of antibacterial agents on Mycoplasma agalactiae, Mycoplasma putrefaciens, Mycoplasma capricolum subsp capricolum, and Mycoplasma mycoides subsp mycoides large colony type. Antimicrobial Agents and Chemotherapy, 50(8), 2845-2849. https://doi.org/10.1128/AAC.01582-05

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title = "Flow cytometric determination of the effects of antibacterial agents on Mycoplasma agalactiae, Mycoplasma putrefaciens, Mycoplasma capricolum subsp capricolum, and Mycoplasma mycoides subsp mycoides large colony type",

abstract = "Flow cytometry together with SYBR green I and propidium iodide was used to study the effects of enrofloxacin, ciprofloxacin, gentamicin, chloramphenicol, oxytetracycline, and tylosin on four mycoplasma species. Inhibition of mycoplasma growth could be detected by as early as 3 h after the start of treatment. The strongest effect was observed with enrofloxacin- and ciprofloxacin-treated cells.",

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Assunção, P, Antunes, NT, Rosales, RS, Poveda, C, Poveda, JB & Davey, HM 2006, 'Flow cytometric determination of the effects of antibacterial agents on Mycoplasma agalactiae, Mycoplasma putrefaciens, Mycoplasma capricolum subsp capricolum, and Mycoplasma mycoides subsp mycoides large colony type', Antimicrobial Agents and Chemotherapy, vol. 50, no. 8, pp. 2845-2849. https://doi.org/10.1128/AAC.01582-05

Flow cytometric determination of the effects of antibacterial agents on Mycoplasma agalactiae, Mycoplasma putrefaciens, Mycoplasma capricolum subsp capricolum, and Mycoplasma mycoides subsp mycoides large colony type. / Assunção, Patrícia; Antunes, Nuno T.; Rosales, Ruben S. et al.
In: Antimicrobial Agents and Chemotherapy, Vol. 50, No. 8, 13.05.2006, p. 2845-2849.

Research output: Contribution to journal › Article › peer-review

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Assunção P, Antunes NT, Rosales RS, Poveda C, Poveda JB, Davey HM. Flow cytometric determination of the effects of antibacterial agents on Mycoplasma agalactiae, Mycoplasma putrefaciens, Mycoplasma capricolum subsp capricolum, and Mycoplasma mycoides subsp mycoides large colony type. Antimicrobial Agents and Chemotherapy. 2006 May 13;50(8):2845-2849. doi: 10.1128/AAC.01582-05