Gender-associated gene expression in two related strains of Schistosoma japonicum

Jennifer M Fitzpatrick, Maria Vang Johansen, David A Johnston, David W Dunne, Karl F Hoffmann

Research output: Contribution to journalArticlepeer-review

70 Citations (SciVal)

Abstract

Host inflammatory responses directed against eggs laid by sexually-mature Schistosoma japonicum female worms instigate lesion formation and associated clinical pathologies during infection. To identify parasite gene transcripts that associate with egg production and to characterise sexually-mature adult gene expression profiles of two related Chinese strains, S. japonicum cDNA microarrays were fabricated using 457 ESTs originating from three parasite developmental stages. Twenty-two female-associated and 8 male-associated gene transcripts were identified in the adult Anhui strain whereas 21 female-associated and 7 male-associated gene transcripts were revealed in the adult Zhejiang strain. RT-PCR analysis, in situ enzyme localisation studies and enzymatic assays confirmed the cDNA microarray results, and importantly, provided information previously unappreciated in schistosome conjugal biology. Specifically, our novel findings include the female-specific expression of genes putatively involved in haemoglobin digestion and eggshell formation including extracellular superoxide dismutase, two histidine-rich proteins, a large blood-brain barrier amino acid transporter and two tyrosinase orthologues. In contrast, transcripts involved in mechanical support (actin), cytoskeletal infrastructure (e.g. dynein light chain 3 and myosin regulatory light chain) and tegumental biology (e.g. TM4SF and Sj25) were more highly represented in adult male schistosomes. Together these data establish a transcriptional basis for adult schistosome labour division and expands the list of novel S. japonicum gender-associated gene transcripts that may be considered targets for improved intervention strategies.
Original languageEnglish
Pages (from-to)191-209
Number of pages19
JournalMolecular and Biochemical Parasitology
Volume136
Issue number2
Early online date13 May 2004
DOIs
Publication statusPublished - Aug 2004

Keywords

  • schistosome
  • gene expression
  • microarray

Fingerprint

Dive into the research topics of 'Gender-associated gene expression in two related strains of Schistosoma japonicum'. Together they form a unique fingerprint.

Cite this