Gene marksmanship access to quality HMW subunit gene expression in transgenic wheat

To explore the feasibility of using genetic engineering techniques for quality improvement of wheat by particle bombardment of Hubei Province three varieties of wheat E - en No. 1, Emai 11 and Emai 12 were high-molecular-weight glutenin subunits (HMW- GS) gene 1Ax1 and 1Dx5 + 1Dy10 transduction. The results showed that the frequency of regeneration and transformation frequency by the bombardment of immature embryo explants was significantly higher than that of young panicle; differences among genotypes callus regeneration capacity and frequency conversion, a direct impact on the success rate of wheat transformation; 3 immature embryo development period and the frequency of regeneration showed a significant negative correlation varieties (r = -0.93, or -0.95); under controlled environmental conditions, the test plants were immature embryo transformation frequency 12 ~ 14 d after flowering, the highest (E Well No. 1, Emai 11 and Emai 12, respectively, 4.5%, 2.9% and 2%). Studies have shown that E Well No. 1, Emai on the 11th age of immature embryos tested materials, with advantages subunit gene 1Dx5 molar ratio and a selectable marker gene and 1Dy10 2: 1 molar ratio of precipitate wrapped powder, dried after 600 ~ 900 psi pressure helium bombardment, callus induction on MS medium containing 0.5 mg · L-1 2,4-D's, containing 0.1 mg · L-1 2,4-D and 5 mg · L-1 differentiated zeatin R media, 3mg · L-1 L-PPT selection pressure subculture differentiation filter, to obtain a stable fertile T0 transgenic plants and endosperm-specific expression of T1 transgenic seeds.