Gene silencing in the liver fluke Fasciola hepatica: RNA interference

Gabriel Rinaldi*, Nicolás Dell’Oca, Estela Castillo, José F. Tort

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (SciVal)

Abstract

The chronic infection with the liver fluke of the genus Fasciola spp. is the most prevalent foodborne trematodiasis, affecting at least one-fourth of the world livestock grazing in areas where the parasite is present. Moreover, fascioliasis is considered a major zoonosis mainly in rural areas of central South America, Northern Africa, and Central Asia. Increasing evidences of resistance against triclabendazole may compromise its use as drug of choice; thus, novel control strategies are desperately needed. Functional genomic approaches play a key role in the validation and characterization of new targets for drug and vaccine development. So far, RNA interference has been the only gene silencing approach successfully employed in liver flukes of the genus Fasciola spp. Herein, we describe a detailed step-by-step protocol to perform gene silencing mediated by RNAi in Fasciola hepatica.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
EditorsMartin Cancela, Gabriela Maggioli
PublisherHumana Press Inc.
Pages67-92
Number of pages26
ISBN (Electronic)9781071604755
ISBN (Print)9781071604748
DOIs
Publication statusPublished - 2020
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2137
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • dsRNA
  • Electrosoaking
  • F. hepatica
  • Gene silencing
  • RNAi
  • siRNA
  • Soaking

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