Projects per year
Abstract
In this study, genetic diversity among 177 oat (Avena sativa L.) accessions including both white and red oat landraces and 36 commercial cultivars was studied for simple sequence repeat (SSR) loci. Thirty-one genomic and expressed sequence tags (EST)-derived primer pairs were selected according to high polymorphism from an initial 66 SSR batch. Markers revealed a high level of polymorphism, detecting a total of 454 alleles. The average gene diversity for the whole sample was 0.29. Genetic similarity, calculated using the Dice coefficient, was used for cluster analysis, and principal component analysis was also applied. In addition, population structure using a Bayesian clustering approach identified discrete subpopulation based on allele frequency and showed similar clustering of oat genotypes in four groups. Accessions could be classified into four main clusters that clearly separated the commercial cultivars, the red oat landraces and two clusters of white oat landraces. Cultivars showed less diversity than the landraces indicating a reduction of genetic diversity during breeding, whereas white oat landraces showed higher diversity than red ones. The average polymorphic information content of 0.80 for the SSR loci indicated the usefulness of many of the SSR for genotype identification. In particular, two markers, MAMA5 and AM04, with a total of 50 alleles and a high discrimination power (>0.90), were sufficient to discriminate among all commercial cultivars studied highlighting their potential use for variety identification.
Original language | English |
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Pages (from-to) | 1305-1314 |
Number of pages | 10 |
Journal | Plant Molecular Biology Reporter |
Volume | 31 |
Issue number | 6 |
Early online date | 09 May 2013 |
DOIs | |
Publication status | Published - 01 Dec 2013 |
Keywords
- A. sativa
- Genetic diversity
- Oat
- Simple sequence repeat (SSR)
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Dive into the research topics of 'Genetic Diversity and Population Structure Among Oat Cultivars and Landraces'. Together they form a unique fingerprint.Projects
- 3 Finished
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Developing tools and resources for oat breeding
Armstead, I. (PI)
01 Apr 2012 → 31 Mar 2017
Project: Externally funded research
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Generation of Oat varieties with enhanced resistance to crown rust and mildew
Marshall, A. (PI)
Engineering and Physical Sciences Research Council
10 Oct 2010 → 30 Sept 2015
Project: Externally funded research
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Harnessing new technologies for sustainable oat production and utilisation (QUOATS)
Marshall, A. (PI)
Biotechnology and Biological Sciences Research Council
14 Sept 2009 → 13 Sept 2014
Project: Externally funded research