Projects per year
Abstract
Key message: Publically available SNP array increases the marker density for genotyping of forage crop,Lolium perenne. Applied to 90 European ecotypes composed of 716 individuals identifies a significant genetic–geographic correlation. Abstract: Grassland ecosystems are ubiquitous across temperate and tropical regions, totalling 37 % of the terrestrial land cover of the planet, and thus represent a global resource for understanding local adaptations to environment. However, genomic resources for grass species (outside cereals) are relatively poor. The advent of next-generation DNA sequencing and high-density SNP genotyping platforms enables the development of dense marker assays for population genetics analyses and genome-wide association studies. A high-density SNP marker resource (Illumina Infinium assay) for perennial ryegrass (Lolium perenne) was created and validated in a broad ecotype collection of 716 individuals sampled from 90 sites across Europe. Genetic diversity within and between populations was assessed. A strong correlation of geographic origin to genetic structure was found using principal component analysis, with significant correlation to longitude and latitude (P < 0.001). The potential of this array as a resource for studies of germplasm diversity and identifying traits underpinning adaptive variation is highlighted.
Original language | English |
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Pages (from-to) | 1917-1932 |
Number of pages | 16 |
Journal | Theoretical and Applied Genetics |
Volume | 128 |
Issue number | 10 |
Early online date | 21 Jun 2015 |
DOIs | |
Publication status | Published - 24 Oct 2015 |
Keywords
- DNA, Plant/genetics
- Ecotype
- Europe
- Genetic Markers
- Genetics, Population
- Genotype
- Genotyping Techniques
- Geography
- Lolium/genetics
- Oligonucleotide Array Sequence Analysis
- Polymorphism, Single Nucleotide
- Sequence Analysis, DNA
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Projects
- 2 Finished
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FACCE ERA-NET+ GrassLandscape (Project Leader: Jean-Paul Sampoux, INRA, France)
Hegarty, M. (PI)
Biotechnology and Biological Sciences Research Council
01 Dec 2014 → 31 Jul 2018
Project: Externally funded research
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C3G: Lolium and Trifolium genetics, genomics and germplasm development
Armstead, I. (PI), Jenkins, G. (PI), Marshall, A. (PI), Skot, L. (PI), Thomas, I. (PI) & Thorogood, D. (PI)
01 Apr 2012 → 31 Mar 2017
Project: Externally funded research