TY - JOUR
T1 - Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.)
AU - de Gyves, Emilio Mendoza
AU - Sparks, Caroline A
AU - Sayanova, Olga
AU - Lazzeri, Paul
AU - Napier, Johnathan A
AU - Jones, Huw D.
PY - 2004/7
Y1 - 2004/7
N2 - A robust Agrobacterium-mediated transformation procedure was developed for Rigel, a commercial cultivar of evening primrose, and used to deliver a cDNA encoding a Delta(6)-desaturase from borage under the control of a cauliflower mosaic virus (CaMV) 35S promoter. Analysis of the transformed plants demonstrated an altered profile of polyunsaturated fatty acids, with an increase in gamma-linolenic acid and octadecatetraenoic acid in leaf tissues when compared with control lines.
AB - A robust Agrobacterium-mediated transformation procedure was developed for Rigel, a commercial cultivar of evening primrose, and used to deliver a cDNA encoding a Delta(6)-desaturase from borage under the control of a cauliflower mosaic virus (CaMV) 35S promoter. Analysis of the transformed plants demonstrated an altered profile of polyunsaturated fatty acids, with an increase in gamma-linolenic acid and octadecatetraenoic acid in leaf tissues when compared with control lines.
UR - http://hdl.handle.net/2160/42790
U2 - 10.1111/j.1467-7652.2004.00079.x
DO - 10.1111/j.1467-7652.2004.00079.x
M3 - Article
C2 - 17134396
SN - 1467-7644
VL - 2
SP - 351
EP - 357
JO - Plant Biotechnology Journal
JF - Plant Biotechnology Journal
IS - 4
ER -