Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.)

Emilio Mendoza de Gyves, Caroline A Sparks, Olga Sayanova, Paul Lazzeri, Johnathan A Napier, Huw D. Jones

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)
124 Downloads (Pure)

Abstract

A robust Agrobacterium-mediated transformation procedure was developed for Rigel, a commercial cultivar of evening primrose, and used to deliver a cDNA encoding a Delta(6)-desaturase from borage under the control of a cauliflower mosaic virus (CaMV) 35S promoter. Analysis of the transformed plants demonstrated an altered profile of polyunsaturated fatty acids, with an increase in gamma-linolenic acid and octadecatetraenoic acid in leaf tissues when compared with control lines.

Original languageEnglish
Pages (from-to)351-7
Number of pages7
JournalPlant Biotechnology Journal
Volume2
Issue number4
DOIs
Publication statusPublished - Jul 2004

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