Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.)

Emilio Mendoza de Gyves; Caroline A Sparks; Olga Sayanova; Paul Lazzeri; Johnathan A Napier; Huw D. Jones

doi:10.1111/j.1467-7652.2004.00079.x

Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.)

Emilio Mendoza de Gyves
, Caroline A Sparks
, Olga Sayanova
, Paul Lazzeri
, Johnathan A Napier
, Huw D. Jones

Institute of Biological, Environmental, and Rural Sciences

Rothamsted Research

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

180 Downloads (Pure)

Abstract

A robust Agrobacterium-mediated transformation procedure was developed for Rigel, a commercial cultivar of evening primrose, and used to deliver a cDNA encoding a Delta(6)-desaturase from borage under the control of a cauliflower mosaic virus (CaMV) 35S promoter. Analysis of the transformed plants demonstrated an altered profile of polyunsaturated fatty acids, with an increase in gamma-linolenic acid and octadecatetraenoic acid in leaf tissues when compared with control lines.

Original language	English
Pages (from-to)	351-7
Number of pages	7
Journal	Plant Biotechnology Journal
Volume	2
Issue number	4
DOIs	https://doi.org/10.1111/j.1467-7652.2004.00079.x
Publication status	Published - Jul 2004

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Genetic manipulation of γ-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.)Final published version, 162 KB

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title = "Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.)",

abstract = "A robust Agrobacterium-mediated transformation procedure was developed for Rigel, a commercial cultivar of evening primrose, and used to deliver a cDNA encoding a Delta(6)-desaturase from borage under the control of a cauliflower mosaic virus (CaMV) 35S promoter. Analysis of the transformed plants demonstrated an altered profile of polyunsaturated fatty acids, with an increase in gamma-linolenic acid and octadecatetraenoic acid in leaf tissues when compared with control lines.",

author = "\{de Gyves\}, \{Emilio Mendoza\} and Sparks, \{Caroline A\} and Olga Sayanova and Paul Lazzeri and Napier, \{Johnathan A\} and Jones, \{Huw D.\}",

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T1 - Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.)

AU - de Gyves, Emilio Mendoza

AU - Sparks, Caroline A

AU - Sayanova, Olga

AU - Lazzeri, Paul

AU - Napier, Johnathan A

AU - Jones, Huw D.

PY - 2004/7

Y1 - 2004/7

N2 - A robust Agrobacterium-mediated transformation procedure was developed for Rigel, a commercial cultivar of evening primrose, and used to deliver a cDNA encoding a Delta(6)-desaturase from borage under the control of a cauliflower mosaic virus (CaMV) 35S promoter. Analysis of the transformed plants demonstrated an altered profile of polyunsaturated fatty acids, with an increase in gamma-linolenic acid and octadecatetraenoic acid in leaf tissues when compared with control lines.

AB - A robust Agrobacterium-mediated transformation procedure was developed for Rigel, a commercial cultivar of evening primrose, and used to deliver a cDNA encoding a Delta(6)-desaturase from borage under the control of a cauliflower mosaic virus (CaMV) 35S promoter. Analysis of the transformed plants demonstrated an altered profile of polyunsaturated fatty acids, with an increase in gamma-linolenic acid and octadecatetraenoic acid in leaf tissues when compared with control lines.

UR - http://hdl.handle.net/2160/42790

U2 - 10.1111/j.1467-7652.2004.00079.x

DO - 10.1111/j.1467-7652.2004.00079.x

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JO - Plant Biotechnology Journal

JF - Plant Biotechnology Journal

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ER -

Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.)

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