Genetic manipulation of schistosomes - Progress with integration competent vectors

Sutas Suttiprapa; Gabriel Rinaldi; Paul J. Brindley

doi:10.1017/S003118201100134X

Genetic manipulation of schistosomes - Progress with integration competent vectors

Sutas Suttiprapa, Gabriel Rinaldi, Paul J. Brindley^*

^*Corresponding author for this work

Research output: Contribution to journal › Review Article › peer-review

14 Citations (Scopus)

Abstract

Draft genome sequences for Schistosoma japonicum and S. mansoni are now available. The schistosome genome encodes ∼13 000 protein-encoding genes for which the functions of few are well understood. Nonetheless, the new genes represent potential intervention targets, and molecular tools are being developed to determine their importance. Over the past 15 years, noteworthy progress has been achieved towards development of tools for gene manipulation and transgenesis of schistosomes. A brief history of genetic manipulation is presented, along with a review of the field with emphasis on reports of integration of transgenes into schistosome chromosomes.

Original language	English
Pages (from-to)	641-650
Number of pages	10
Journal	Parasitology
Volume	139
Issue number	5
DOIs	https://doi.org/10.1017/S003118201100134X
Publication status	Published - Apr 2012
Externally published	Yes

Keywords

chromosome integration
genetic manipulation
germ line
murine leukaemia virus
piggyBac
pseudotyped gammaretrovirus
retrovirus
Schistosomes
transgenesis
transposon
Gene Transfer Techniques
Schistosoma/genetics
Animals
Life Cycle Stages/genetics
RNA Interference
Genetic Vectors/genetics
Transgenes/genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1017/S003118201100134X

Permanent link

Persistent link

Cite this

@article{533de1c7b19b4c88a48bb98c4d3f3f3f,

title = "Genetic manipulation of schistosomes - Progress with integration competent vectors",

abstract = "Draft genome sequences for Schistosoma japonicum and S. mansoni are now available. The schistosome genome encodes ∼13 000 protein-encoding genes for which the functions of few are well understood. Nonetheless, the new genes represent potential intervention targets, and molecular tools are being developed to determine their importance. Over the past 15 years, noteworthy progress has been achieved towards development of tools for gene manipulation and transgenesis of schistosomes. A brief history of genetic manipulation is presented, along with a review of the field with emphasis on reports of integration of transgenes into schistosome chromosomes.",

keywords = "chromosome integration, genetic manipulation, germ line, murine leukaemia virus, piggyBac, pseudotyped gammaretrovirus, retrovirus, Schistosomes, transgenesis, transposon, Gene Transfer Techniques, Schistosoma/genetics, Animals, Life Cycle Stages/genetics, RNA Interference, Genetic Vectors/genetics, Transgenes/genetics",

author = "Sutas Suttiprapa and Gabriel Rinaldi and Brindley, {Paul J.}",

year = "2012",

month = apr,

doi = "10.1017/S003118201100134X",

language = "English",

volume = "139",

pages = "641--650",

journal = "Parasitology",

issn = "0031-1820",

publisher = "Cambridge University Press",

number = "5",

}

TY - JOUR

T1 - Genetic manipulation of schistosomes - Progress with integration competent vectors

AU - Suttiprapa, Sutas

AU - Rinaldi, Gabriel

AU - Brindley, Paul J.

PY - 2012/4

Y1 - 2012/4

N2 - Draft genome sequences for Schistosoma japonicum and S. mansoni are now available. The schistosome genome encodes ∼13 000 protein-encoding genes for which the functions of few are well understood. Nonetheless, the new genes represent potential intervention targets, and molecular tools are being developed to determine their importance. Over the past 15 years, noteworthy progress has been achieved towards development of tools for gene manipulation and transgenesis of schistosomes. A brief history of genetic manipulation is presented, along with a review of the field with emphasis on reports of integration of transgenes into schistosome chromosomes.

AB - Draft genome sequences for Schistosoma japonicum and S. mansoni are now available. The schistosome genome encodes ∼13 000 protein-encoding genes for which the functions of few are well understood. Nonetheless, the new genes represent potential intervention targets, and molecular tools are being developed to determine their importance. Over the past 15 years, noteworthy progress has been achieved towards development of tools for gene manipulation and transgenesis of schistosomes. A brief history of genetic manipulation is presented, along with a review of the field with emphasis on reports of integration of transgenes into schistosome chromosomes.

KW - chromosome integration

KW - genetic manipulation

KW - germ line

KW - murine leukaemia virus

KW - piggyBac

KW - pseudotyped gammaretrovirus

KW - retrovirus

KW - Schistosomes

KW - transgenesis

KW - transposon

KW - Gene Transfer Techniques

KW - Schistosoma/genetics

KW - Animals

KW - Life Cycle Stages/genetics

KW - RNA Interference

KW - Genetic Vectors/genetics

KW - Transgenes/genetics

UR - http://www.scopus.com/inward/record.url?scp=84859386551&partnerID=8YFLogxK

U2 - 10.1017/S003118201100134X

DO - 10.1017/S003118201100134X

M3 - Review Article

C2 - 21854676

AN - SCOPUS:84859386551

SN - 0031-1820

VL - 139

SP - 641

EP - 650

JO - Parasitology

JF - Parasitology

IS - 5

ER -

Genetic manipulation of schistosomes - Progress with integration competent vectors

Abstract

Keywords

UN SDGs

Access to Document

Permanent link

Other files and links

Fingerprint

Cite this