Genome Sequence Analysis of Two Pseudomonas putida Strains to Identify a 17-Hydroxylase Putatively Involved in Sparteine Degradation

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Abstract

Two strains of Pseudomonas putida, Psp-LUP and Psp-SPAR, capable of growth on the quinolizidine alkaloids, lupanine and sparteine respectively, were studied here. We report the isolation of Psp-SPAR and the complete genome sequencing of both bacteria. Both were confirmed to belong to P. putida, Psp-LUP close to the type isolate of the species (NBRC14164T) and Psp-SPAR close to strains KT2440 and F1. Psp-SPAR did not grow on lupanine but did contain a gene encoding a putative quinolizidine-17-hydroxylase peptide which exhibited high similarity (76%identity) to the lupanine-17-hydroxylase characterised from Psp-LUP.
Original languageEnglish
Pages (from-to)1649-1654
Number of pages6
JournalCurrent Microbiology
Volume75
Issue number12
Early online date28 Sept 2018
DOIs
Publication statusPublished - 01 Dec 2018

Keywords

  • Alkaloids/genetics
  • Bacterial Proteins/genetics
  • Genome, Bacterial/genetics
  • Oxidoreductases Acting on CH-NH Group Donors/genetics
  • Pseudomonas putida/genetics
  • Sequence Analysis/methods
  • Sparteine/analogs & derivatives
  • Steroid 17-alpha-Hydroxylase/genetics
  • Whole Genome Sequencing/methods

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