Genome Sequence Analysis of Two Pseudomonas putida Strains to Identify a 17-Hydroxylase Putatively Involved in Sparteine Degradation

Andrew Detheridge; Gareth Griffith; David Hopper

doi:10.1007/s00284-018-1573-2

Genome Sequence Analysis of Two Pseudomonas putida Strains to Identify a 17-Hydroxylase Putatively Involved in Sparteine Degradation

Institute of Biological, Environmental, and Rural Sciences

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Abstract

Two strains of Pseudomonas putida, Psp-LUP and Psp-SPAR, capable of growth on the quinolizidine alkaloids, lupanine and sparteine respectively, were studied here. We report the isolation of Psp-SPAR and the complete genome sequencing of both bacteria. Both were confirmed to belong to P. putida, Psp-LUP close to the type isolate of the species (NBRC14164T) and Psp-SPAR close to strains KT2440 and F1. Psp-SPAR did not grow on lupanine but did contain a gene encoding a putative quinolizidine-17-hydroxylase peptide which exhibited high similarity (76%identity) to the lupanine-17-hydroxylase characterised from Psp-LUP.

Original language	English
Pages (from-to)	1649-1654
Number of pages	6
Journal	Current Microbiology
Volume	75
Issue number	12
Early online date	28 Sept 2018
DOIs	https://doi.org/10.1007/s00284-018-1573-2
Publication status	Published - 01 Dec 2018

Keywords

Alkaloids/genetics
Bacterial Proteins/genetics
Genome, Bacterial/genetics
Oxidoreductases Acting on CH-NH Group Donors/genetics
Pseudomonas putida/genetics
Sequence Analysis/methods
Sparteine/analogs & derivatives
Steroid 17-alpha-Hydroxylase/genetics
Whole Genome Sequencing/methods

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10.1007/s00284-018-1573-2Licence: CC BY

Genome Sequence Analysis of Two Pseudomonas putida Strains to Identify a 17-Hydroxylase Putatively Involved in Sparteine DegradationFinal published version, 1.13 MBLicence: CC BY

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title = "Genome Sequence Analysis of Two Pseudomonas putida Strains to Identify a 17-Hydroxylase Putatively Involved in Sparteine Degradation",

abstract = "Two strains of Pseudomonas putida, Psp-LUP and Psp-SPAR, capable of growth on the quinolizidine alkaloids, lupanine and sparteine respectively, were studied here. We report the isolation of Psp-SPAR and the complete genome sequencing of both bacteria. Both were confirmed to belong to P. putida, Psp-LUP close to the type isolate of the species (NBRC14164T) and Psp-SPAR close to strains KT2440 and F1. Psp-SPAR did not grow on lupanine but did contain a gene encoding a putative quinolizidine-17-hydroxylase peptide which exhibited high similarity (76\%identity) to the lupanine-17-hydroxylase characterised from Psp-LUP.",

keywords = "Alkaloids/genetics, Bacterial Proteins/genetics, Genome, Bacterial/genetics, Oxidoreductases Acting on CH-NH Group Donors/genetics, Pseudomonas putida/genetics, Sequence Analysis/methods, Sparteine/analogs \& derivatives, Steroid 17-alpha-Hydroxylase/genetics, Whole Genome Sequencing/methods",

author = "Andrew Detheridge and Gareth Griffith and David Hopper",

note = "Funding Information: The complete genome sequences were deposited in the European Nucelotide Archive (https://www.ebi.ac.uk/ena) with the accession number for Pseudomonas putida (LUP) of ERX2741807 (PRJEB28091) and for Pseudomonas putida (SPAR) of ERX2741806 (PRJEB28090). Genome sequencing was provided by MicrobesNG (http://www.microbesng .uk), which is supported by the BBSRC (grant number BB/ L024209/1).Both cultures are deposited at the National Collection of Industrial, Food and Marine Bacteria (NCIMB Ltd., Aberdeen) with accession numbers 15126 (Psp-LUP) Publisher Copyright: {\textcopyright} 2018, The Author(s).",

year = "2018",

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doi = "10.1007/s00284-018-1573-2",

language = "English",

volume = "75",

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T1 - Genome Sequence Analysis of Two Pseudomonas putida Strains to Identify a 17-Hydroxylase Putatively Involved in Sparteine Degradation

AU - Detheridge, Andrew

AU - Griffith, Gareth

AU - Hopper, David

N1 - Funding Information: The complete genome sequences were deposited in the European Nucelotide Archive (https://www.ebi.ac.uk/ena) with the accession number for Pseudomonas putida (LUP) of ERX2741807 (PRJEB28091) and for Pseudomonas putida (SPAR) of ERX2741806 (PRJEB28090). Genome sequencing was provided by MicrobesNG (http://www.microbesng .uk), which is supported by the BBSRC (grant number BB/ L024209/1).Both cultures are deposited at the National Collection of Industrial, Food and Marine Bacteria (NCIMB Ltd., Aberdeen) with accession numbers 15126 (Psp-LUP) Publisher Copyright: © 2018, The Author(s).

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Two strains of Pseudomonas putida, Psp-LUP and Psp-SPAR, capable of growth on the quinolizidine alkaloids, lupanine and sparteine respectively, were studied here. We report the isolation of Psp-SPAR and the complete genome sequencing of both bacteria. Both were confirmed to belong to P. putida, Psp-LUP close to the type isolate of the species (NBRC14164T) and Psp-SPAR close to strains KT2440 and F1. Psp-SPAR did not grow on lupanine but did contain a gene encoding a putative quinolizidine-17-hydroxylase peptide which exhibited high similarity (76%identity) to the lupanine-17-hydroxylase characterised from Psp-LUP.

AB - Two strains of Pseudomonas putida, Psp-LUP and Psp-SPAR, capable of growth on the quinolizidine alkaloids, lupanine and sparteine respectively, were studied here. We report the isolation of Psp-SPAR and the complete genome sequencing of both bacteria. Both were confirmed to belong to P. putida, Psp-LUP close to the type isolate of the species (NBRC14164T) and Psp-SPAR close to strains KT2440 and F1. Psp-SPAR did not grow on lupanine but did contain a gene encoding a putative quinolizidine-17-hydroxylase peptide which exhibited high similarity (76%identity) to the lupanine-17-hydroxylase characterised from Psp-LUP.

KW - Alkaloids/genetics

KW - Bacterial Proteins/genetics

KW - Genome, Bacterial/genetics

KW - Oxidoreductases Acting on CH-NH Group Donors/genetics

KW - Pseudomonas putida/genetics

KW - Sequence Analysis/methods

KW - Sparteine/analogs & derivatives

KW - Steroid 17-alpha-Hydroxylase/genetics

KW - Whole Genome Sequencing/methods

UR - https://www.scopus.com/pages/publications/85054063760

U2 - 10.1007/s00284-018-1573-2

DO - 10.1007/s00284-018-1573-2

M3 - Article

C2 - 30267141

SN - 0343-8651

VL - 75

SP - 1649

EP - 1654

JO - Current Microbiology

JF - Current Microbiology

IS - 12

ER -

Genome Sequence Analysis of Two Pseudomonas putida Strains to Identify a 17-Hydroxylase Putatively Involved in Sparteine Degradation

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