Greenhouse Spatial Effects Detected in the Barley (Hordeum vulgare L.) Epigenome Underlie Stochasticity of DNA Methylation

Moumouni Konate, Michael J. Wilkinson*, Julian Taylor, Eileen S. Scott, Bettina Berger, Carlos Marcelino Rodriguez Lopez

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (SciVal)
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Abstract

Environmental cues are known to alter the methylation profile of genomic DNA, and thereby change the expression of some genes. A proportion of such modifications may become adaptive by adjusting expression of stress response genes but others have been shown to be highly stochastic, even under controlled conditions. The influence of environmental flux on plants adds an additional layer of complexity that has potential to confound attempts to interpret interactions between environment, methylome, and plant form. We therefore adopt a positional and longitudinal approach to study progressive changes to barley DNA methylation patterns in response to salt exposure during development under greenhouse conditions. Methylation-sensitive amplified polymorphism (MSAP) and phenotypic analyses of nine diverse barley varieties were grown in a randomized plot design, under two salt treatments (0 and 75 mM NaCl). Combining environmental, phenotypic and epigenetic data analyses, we show that at least part of the epigenetic variability, previously described as stochastic, is linked to environmental micro-variations during plant growth. Additionally, we show that differences in methylation increase with time of exposure to micro-variations in environment. We propose that subsequent epigenetic studies take into account microclimate-induced epigenetic variability.

Original languageEnglish
Article number553907
Number of pages15
JournalFrontiers in Plant Science
Volume11
DOIs
Publication statusPublished - 10 Sept 2020

Keywords

  • epigenetics
  • genome by environment
  • methylation-sensitive amplified polymorphism
  • phenotypic plasticity
  • positional effect
  • salt stress

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