Projects per year
Abstract
Nitrogen and water are the primary resources limiting agricultural production worldwide. We have demonstrated the ability of a novel halotolerant bacterial endophyte, Bacillus velezensis CBE, to induce osmotic stress tolerance in Brachypodium distachyon under nitrogen-deprived conditions. Additionally, we aimed to identify the molecular factors in plants that contribute to the beneficial effects induced by B. velezensis CBE in B. distachyon. To achieve this, we conducted transcriptomic profiling using RNA-seq on 18-day-old B. distachyon seedlings treated with B. velezensis CBE in the presence or absence of available nitrogen, with and without osmotic stress. These profiles were then compared to those obtained from B. distachyon treated with known plant growth-promoting bacterial strains, Azospirillum brasilense Cd and Azoarcus olearius DQS4, under the same growth conditions. We identified differentially expressed genes (DEGs) in response to the combinations of bacterial strains and stress treatments. Interestingly, only 73 transcripts showed significant differential expression in B. velezensis CBE-treated plants under stress conditions, compared to 1,078 DEGs in plants treated with A. brasilense Cd and 2,015 DEGs in A. olearius DQS4. Our findings suggest that the novel endophyte B. velezensis CBE mediates osmotic stress tolerance in B. distachyon through the fine-tuning of molecular mechanisms with minimal transcriptional modifications.
Original language | English |
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Article number | 1485391 |
Number of pages | 16 |
Journal | Frontiers in Plant Science |
Volume | 15 |
DOIs | |
Publication status | Published - 10 Jan 2025 |
Keywords
- abiotic stress
- transcription
- endophytes
- tolerance
- beneficial microbes
Fingerprint
Dive into the research topics of 'Halotolerant bacterial endophyte Bacillus velezensis CBE mediates abiotic stress tolerance with minimal transcriptional modifications in Brachypodium distachyon'. Together they form a unique fingerprint.Projects
- 4 Finished
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BBSRC Core Strategic Programme in Resilient Crops: Miscanthus
Donnison, I. (PI)
Biotechnology and Biological Sciences Research Council
01 Apr 2017 → 31 Mar 2020
Project: Externally funded research
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RCUK-CONFAP Newton Fund Research Partnership Award BB/M029271/1 - Brazilian Funds
Farrar, K. (PI)
Biotechnology and Biological Sciences Research Council
15 Oct 2016 → 29 Dec 2016
Project: Externally funded research
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Understanding and Exploiting Biological Nitrogen Fixation for improvement of Brazilian Agriculture
Farrar, K. (PI)
Biotechnology and Biological Sciences Research Council
01 Feb 2016 → 30 Sept 2019
Project: Externally funded research
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Comparative genomic and physiological analysis of beneficial C4 plant-microbe symbiosis for biological nitrogen fixation, drought tolerance and sustainable agriculture
Farrar, K. (PI)
01 Jan 2015 → 30 Sept 2018
Project: Externally funded research
Press/Media
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Institute of Biological Research Researchers Release New Study Findings on Plant Science (Halotolerant bacterial endophyte Bacillus velezensis CBE mediates abiotic stress tolerance with minimal transcriptional modifications in Brachypodium ...)
Fernandez Fuentes, N. & Farrar, K.
28 Jan 2025
1 item of Media coverage
Press/Media: Media coverage