TY - JOUR
T1 - High-resolution organellar genome analysis of Triticum and Aegilops sheds new light on cytoplasm evolution in wheat
AU - Provan, J
AU - Wolters, P
AU - Caldwell, KH
AU - Powell, W
N1 - Provan, J., Wolters, P., Caldwell, K. S., Powell, W. (2004).High-resolution organellar genome analysis of Triticum and Aegilops sheds new light on cytoplasm evolution in wheat. Theoretical and Applied Genetics, 108, (6), 1182-1190.
Sponsorship: SEERAD
PY - 2004/11/20
Y1 - 2004/11/20
N2 - We have utilised polymorphic chloroplast microsatellites to analyse cytoplasmic relationships between accessions in the genera Triticum and Aegilops. Sequencing of PCR products revealed point mutations and insertions/deletions in addition to the standard repeat length expansion/contraction which most likely represent ancient synapomorphies. Phylogenetic analyses revealed three distinct groups of accessions. One of these contained all the non-Aegilops speltoides S-type cytoplasm species, another comprised almost exclusively A, C, D, M, N, T and U cytoplasm-type accessions and the third contained the polyploid Triticum species and all the Ae. speltoides accessions, further confirming that Ae. speltoides or a closely related but now extinct species was the original B-genome donor of cultivated polyploid wheat. Successive decreases in levels of genetic diversity due to domestication were also observed. Finally, we highlight the importance of elucidating longer-term evolutionary processes operating at microsatellite repeat loci.
AB - We have utilised polymorphic chloroplast microsatellites to analyse cytoplasmic relationships between accessions in the genera Triticum and Aegilops. Sequencing of PCR products revealed point mutations and insertions/deletions in addition to the standard repeat length expansion/contraction which most likely represent ancient synapomorphies. Phylogenetic analyses revealed three distinct groups of accessions. One of these contained all the non-Aegilops speltoides S-type cytoplasm species, another comprised almost exclusively A, C, D, M, N, T and U cytoplasm-type accessions and the third contained the polyploid Triticum species and all the Ae. speltoides accessions, further confirming that Ae. speltoides or a closely related but now extinct species was the original B-genome donor of cultivated polyploid wheat. Successive decreases in levels of genetic diversity due to domestication were also observed. Finally, we highlight the importance of elucidating longer-term evolutionary processes operating at microsatellite repeat loci.
U2 - 10.1007/s00122-003-1538-z
DO - 10.1007/s00122-003-1538-z
M3 - Article
SN - 1432-2242
VL - 108
SP - 1182
EP - 1190
JO - Theoretical and Applied Genetics
JF - Theoretical and Applied Genetics
IS - 6
ER -