High throughput, non-targeted metabolite fingerprinting using nominal mass Flow Injection Electrospray Mass Spectrometry

Manfred Beckmann, David A Parker, David Pierre Louis Enot, Emilie Duval, John Draper

Research output: Contribution to journalArticle

110 Citations (SciVal)

Abstract

Flow injection electrospray–mass spectrometry (FIE–MS) is finding utility as a first-pass metabolite fingerprinting tool in many research fields. We provide a protocol that has proved reliable in large-scale research projects involving diverse sample matrices originating from plants, microbes and mammalian biofluids. Using Brachypodium leaf material as an example matrix all steps are summarized from sample extraction to data quality assessment. Alternative procedures for dealing with other common matrices such as bloods and urine are included. With little sample pretreatment, no chromatography and instrument cycle times of <5 min it is feasible to analyze >1,000 samples per week. Analysis of a typical batch of 240 samples (including first-pass data analysis) can be accomplished within 48 h.
Original languageEnglish
Pages (from-to)486-504
Number of pages19
JournalNature Protocols
Volume3
DOIs
Publication statusPublished - 28 Feb 2008

Fingerprint

Dive into the research topics of 'High throughput, non-targeted metabolite fingerprinting using nominal mass Flow Injection Electrospray Mass Spectrometry'. Together they form a unique fingerprint.

Cite this