Highly variable SSR markers in Douglas-fir: Mendelian inheritance and map locations

G. T. Slavov*, G. T. Howe, I. Yakovlev, K. J. Edwards, K. V. Krutovskii, G. A. Tuskan, J. E. Carlson, S. H. Strauss, W. T. Adams

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

47 Citations (SciVal)


Twenty-two highly variable SSR markers were developed in Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco] from five SSR-enriched genomic libraries. Fifteen PCR primer pairs amplified a single codominant locus, while seven primer pairs occasionally amplified two loci. The Mendelian inheritance of all 22 SSRs was confirmed via segregation analyses in several Douglas-fir families. The mean observed heterozygosity and the mean number of alleles per locus were 0.855 (SE=0.020) and 23 (SE=1.6), respectively. Twenty markers were used in genetic linkage analysis and mapped to ten known linkage groups. Because of their high polymorphism and unambiguous phenotypes, 15 single-locus markers were selected as the most suitable for DNA fingerprinting and parentage analysis. Only three SSRs were sufficient to achieve an average probability of exclusion from paternity of 0.998 in a Douglas-fir seed orchard block consisting of 59 parents.

Original languageEnglish
Pages (from-to)873-880
Number of pages8
JournalTheoretical and Applied Genetics
Issue number5
Publication statusPublished - Mar 2004


  • Base Sequence
  • DNA Primers
  • Genetic Markers
  • Heterozygote
  • Phenotype
  • Polymorphism, Genetic
  • Trees/genetics


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