Human U6 promoter drives stronger shRNA activity than its schistosome orthologue in Schistosoma mansoni and human fibrosarcoma cells

Raphaël Duvoisin, Mary A. Ayuk, Gabriel Rinaldi, Sutas Suttiprapa, Victoria H. Mann, Clarence M. Lee, Nicola Harris, Paul J. Brindley

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Blood flukes or schistosomes are the causative agents of human schistosomiasis, one of the major neglected tropical diseases. Draft genome sequences have been reported for schistosomes, but functional genomics tools are needed to investigate the role and essentiality of the newly reported genes. Vector based RNA interference can contribute to functional genomics analysis for schistosomes. Using mRNA encoding reporter firefly luciferase as a model target, we compared the performance of a schistosome and a human promoter from the U6 gene in driving shRNA in human fibrosarcoma cells and in cultured schistosomes. Further, both a retroviral [Murine leukemia virus (MLV)] and plasmid (piggyBac, pXL-Bac II) vector were utilized. The schistosome U6 gene promoter was 270 bp in length, the human U6 gene promoter was 264 bp; they shared 41% identity. Following transduction of both HT1080 fibrosarcoma cells and schistosomules of Schistosoma mansoni with pseudotyped MLV virions, stronger knockdown of luciferase activity was seen with the virions encoding the human U6 promoter driven shRNA than the schistosome U6 promoter. A similar trend was seen after transfection of HT1080 cells and schistosomules with the pXL-Bac-II constructs-stronger knockdown of luciferase activity was seen with constructs encoding the human compared to schistosome U6 promoter. The findings indicate that a human U6 gene promoter drives stronger shRNA activity than its schistosome orthologue, not only in a human cancer cell line but also in larval schistosomes. This RNA polymerase III promoter represents a potentially valuable component for vector based RNA interference studies in schistosomes and related platyhelminth parasites.

Original languageEnglish
Pages (from-to)511-521
Number of pages11
JournalTransgenic Research
Volume21
Issue number3
DOIs
Publication statusPublished - Jun 2012
Externally publishedYes

Keywords

  • Firefly luciferase
  • Murine leukemia virus
  • piggyBac transposon
  • Promoter
  • RNA interference
  • Schistosoma mansoni
  • Schistosome
  • Short hairpin RNA
  • U6 gene
  • Humans
  • RNA, Small Interfering/genetics
  • Genes, Helminth
  • Leukemia Virus, Murine/genetics
  • Plasmids/genetics
  • Ribonucleoprotein, U4-U6 Small Nuclear/genetics
  • RNA, Messenger/genetics
  • Transfection
  • RNA Interference
  • Schistosoma mansoni/genetics
  • Genes, Reporter
  • Promoter Regions, Genetic
  • Luciferases, Firefly/genetics
  • Animals
  • Genetic Vectors/genetics
  • Cell Line, Tumor
  • Fibrosarcoma/genetics
  • Enzyme Activation

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