TY - JOUR
T1 - Identification of a lineage negative cell population in bovine peripheral blood with the ability to mount a strong type I interferon response
AU - Gibson, Amanda
AU - Miah, Suhel
AU - Griebel, Philip
AU - Brownlie, Joe
AU - Werling, Dirk
PY - 2012/2/1
Y1 - 2012/2/1
N2 - Lineage negative dendritic cells, or natural interferon-producing cells (NIPC), also referred to as plasmacytoid dendritic cells (pDC) constitute a small population of leukocytes secreting high levels of type I interferon (IFNα/β) in response to certain danger signals. Here, we provide initial data towards the identification of so far uncharacterised circulating bovine pDC like cells. A lineage negative cell population (LIN - cells) was isolated from PBMC which showed characteristics similar to that of pDC in other species. Isolated LIN - cells presented lymphoid morphology with a semi-crescent nucleus, extensive ER and Golgi network; indicative of pDC. In addition phenotypic analysis of LIN - cells described them as distinct from other bovine DC subsets; expressing both lymphoid and myeloid surface markers. LIN - cells did not express lineage specific markers, but were MHC class II +, CD45RO +, CD80/86 +, CD6 +, WC1 +, CD26 + and expressed the myeloid markers CD205, CD172a and CD11a. In keeping with pDC, LIN - cells express TLR7 mRNA transcripts; however, in a resting state do not express TLR8 or TLR9. Functionally, LIN - cells, but not PBMC, monocytes and monocyte derived DC produce large amounts of IFNα/β in response to different CpG oligonucleotides. Taken together, we present data suggesting that an enriched circulating population of bovine LIN - cells are uniquely capable of producing IFNα/β in response to CpG oligonucleotides and thus this population likely contain the functional equivalent of bovine pDC.
AB - Lineage negative dendritic cells, or natural interferon-producing cells (NIPC), also referred to as plasmacytoid dendritic cells (pDC) constitute a small population of leukocytes secreting high levels of type I interferon (IFNα/β) in response to certain danger signals. Here, we provide initial data towards the identification of so far uncharacterised circulating bovine pDC like cells. A lineage negative cell population (LIN - cells) was isolated from PBMC which showed characteristics similar to that of pDC in other species. Isolated LIN - cells presented lymphoid morphology with a semi-crescent nucleus, extensive ER and Golgi network; indicative of pDC. In addition phenotypic analysis of LIN - cells described them as distinct from other bovine DC subsets; expressing both lymphoid and myeloid surface markers. LIN - cells did not express lineage specific markers, but were MHC class II +, CD45RO +, CD80/86 +, CD6 +, WC1 +, CD26 + and expressed the myeloid markers CD205, CD172a and CD11a. In keeping with pDC, LIN - cells express TLR7 mRNA transcripts; however, in a resting state do not express TLR8 or TLR9. Functionally, LIN - cells, but not PBMC, monocytes and monocyte derived DC produce large amounts of IFNα/β in response to different CpG oligonucleotides. Taken together, we present data suggesting that an enriched circulating population of bovine LIN - cells are uniquely capable of producing IFNα/β in response to CpG oligonucleotides and thus this population likely contain the functional equivalent of bovine pDC.
KW - Bovine
KW - IFNα/β
KW - PDC
KW - Plasmacytoid
KW - TLR
UR - http://www.scopus.com/inward/record.url?scp=80455164670&partnerID=8YFLogxK
U2 - 10.1016/j.dci.2011.05.002
DO - 10.1016/j.dci.2011.05.002
M3 - Article
C2 - 21663757
AN - SCOPUS:80455164670
SN - 0145-305X
VL - 36
SP - 332
EP - 341
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
IS - 2
ER -