Identification of genes expressed during the self-incompatibility response in perennial ryegrass (Lolium perenne L.)

Bicheng Yang; Daniel Thorogood; Ian P. Armstead; F. C. H. Franklin; Susanne Barth

doi:10.1007/s11103-009-9501-2

Identification of genes expressed during the self-incompatibility response in perennial ryegrass (Lolium perenne L.)

Bicheng Yang, Daniel Thorogood, Ian P. Armstead, F. C. H. Franklin, Susanne Barth

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Self-incompatibility (SI) in Lolium perenne is controlled gametophytically by the S–Z two-locus system. S and Z loci mapped to L. perenne linkage groups 1 and 2, respectively, with their corresponding putative-syntenic regions on rice chromosome 5 (R5) and R4. None of the gene products of S and Z have yet been identified. SI cDNA libraries were developed to enrich for SI expressed genes in L. perenne. Transcripts were identified from the SI libraries that were orthologous to sequences on rice R4 and R5. These represent potential SI candidate genes. Altogether ten expressed SI candidate genes were identified. A rapid increase in gene expression within two minutes after pollen-stigma contact was revealed, reaching a maximum between 2 and 10 min. The potential involvement of these genes in the SI reactions is discussed.

Original language	English
Pages (from-to)	709-723
Number of pages	15
Journal	Plant Molecular Biology
Volume	70
Issue number	6
Early online date	30 May 2009
DOIs	https://doi.org/10.1007/s11103-009-9501-2
Publication status	Published - 01 Aug 2009

Keywords

Self-incompatibility (SI)
Lolium perenne
Grasses
S and Z
Suppression subtractive hybridization (SSH)

Access to Document

10.1007/s11103-009-9501-2

http://hdl.handle.net/2160/4569

Permanent link

Persistent link

Cite this

@article{e377cd619039430081132638f548340b,

title = "Identification of genes expressed during the self-incompatibility response in perennial ryegrass (Lolium perenne L.)",

abstract = "Self-incompatibility (SI) in Lolium perenne is controlled gametophytically by the S–Z two-locus system. S and Z loci mapped to L. perenne linkage groups 1 and 2, respectively, with their corresponding putative-syntenic regions on rice chromosome 5 (R5) and R4. None of the gene products of S and Z have yet been identified. SI cDNA libraries were developed to enrich for SI expressed genes in L. perenne. Transcripts were identified from the SI libraries that were orthologous to sequences on rice R4 and R5. These represent potential SI candidate genes. Altogether ten expressed SI candidate genes were identified. A rapid increase in gene expression within two minutes after pollen-stigma contact was revealed, reaching a maximum between 2 and 10 min. The potential involvement of these genes in the SI reactions is discussed.",

keywords = "Self-incompatibility (SI), Lolium perenne, Grasses, S and Z, Suppression subtractive hybridization (SSH)",

author = "Bicheng Yang and Daniel Thorogood and Armstead, {Ian P.} and Franklin, {F. C. H.} and Susanne Barth",

note = "The online version of this article (doi:10.1007/s11103-009-9501-2) contains supplementary material, which is available to authorized users. IMPF: 03.98 RONO: 1340 3002 Sponsorship: Teagasc/National Development Plan.",

year = "2009",

month = aug,

day = "1",

doi = "10.1007/s11103-009-9501-2",

language = "English",

volume = "70",

pages = "709--723",

journal = "Plant Molecular Biology",

issn = "1573-5028",

publisher = "Springer Nature",

number = "6",

}

TY - JOUR

T1 - Identification of genes expressed during the self-incompatibility response in perennial ryegrass (Lolium perenne L.)

AU - Yang, Bicheng

AU - Thorogood, Daniel

AU - Armstead, Ian P.

AU - Franklin, F. C. H.

AU - Barth, Susanne

N1 - The online version of this article (doi:10.1007/s11103-009-9501-2) contains supplementary material, which is available to authorized users. IMPF: 03.98 RONO: 1340 3002 Sponsorship: Teagasc/National Development Plan.

PY - 2009/8/1

Y1 - 2009/8/1

N2 - Self-incompatibility (SI) in Lolium perenne is controlled gametophytically by the S–Z two-locus system. S and Z loci mapped to L. perenne linkage groups 1 and 2, respectively, with their corresponding putative-syntenic regions on rice chromosome 5 (R5) and R4. None of the gene products of S and Z have yet been identified. SI cDNA libraries were developed to enrich for SI expressed genes in L. perenne. Transcripts were identified from the SI libraries that were orthologous to sequences on rice R4 and R5. These represent potential SI candidate genes. Altogether ten expressed SI candidate genes were identified. A rapid increase in gene expression within two minutes after pollen-stigma contact was revealed, reaching a maximum between 2 and 10 min. The potential involvement of these genes in the SI reactions is discussed.

AB - Self-incompatibility (SI) in Lolium perenne is controlled gametophytically by the S–Z two-locus system. S and Z loci mapped to L. perenne linkage groups 1 and 2, respectively, with their corresponding putative-syntenic regions on rice chromosome 5 (R5) and R4. None of the gene products of S and Z have yet been identified. SI cDNA libraries were developed to enrich for SI expressed genes in L. perenne. Transcripts were identified from the SI libraries that were orthologous to sequences on rice R4 and R5. These represent potential SI candidate genes. Altogether ten expressed SI candidate genes were identified. A rapid increase in gene expression within two minutes after pollen-stigma contact was revealed, reaching a maximum between 2 and 10 min. The potential involvement of these genes in the SI reactions is discussed.

KW - Self-incompatibility (SI)

KW - Lolium perenne

KW - Grasses

KW - S and Z

KW - Suppression subtractive hybridization (SSH)

U2 - 10.1007/s11103-009-9501-2

DO - 10.1007/s11103-009-9501-2

M3 - Article

C2 - 19484189

SN - 1573-5028

VL - 70

SP - 709

EP - 723

JO - Plant Molecular Biology

JF - Plant Molecular Biology

IS - 6

ER -

Identification of genes expressed during the self-incompatibility response in perennial ryegrass (Lolium perenne L.)

Abstract

Keywords

Access to Document

Permanent link

Fingerprint

Cite this