Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis

Konstantin P. Lyashchenko, Adrian Grandison, Karen Keskinen, Alina Sikar-gang, Paul Lambotte, Javan Esfandiari, Gregory C. Ireton, Aarthy Vallur, Steven G. Reed, Gareth Jones, H. Martin Vordermeier, Judy R. Stabel, Tyler C. Thacker, Mitchell V. Palmer, W. Ray Waters, Patricia P. Wilkins (Editor)

Research output: Contribution to journalArticlepeer-review

17 Citations (SciVal)

Abstract

Bovine tuberculosis (TB), caused by Mycobacterium bovis, remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we screened a panel of 101 recombinant proteins, including 10 polyepitope fusions, by a multiantigen print immunoassay (MAPIA) with well-characterized serum samples serially collected from cattle with experimental or naturally acquired M. bovis infection. A novel set of 12 seroreactive antigens was established. Evaluation of selected proteins in the dual-path platform (DPP) assay showed that the highest diagnostic accuracy (∼95%) was achieved with a cocktail of five best-performing antigens, thus demonstrating the potential for development of an improved and more practical serodiagnostic test for bovine TB.
Original languageEnglish
Article numbere00259-17
Number of pages9
JournalClinical and Vaccine Immunology
Volume24
Issue number12
DOIs
Publication statusPublished - 05 Dec 2017

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