Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis

Konstantin P. Lyashchenko; Adrian Grandison; Karen Keskinen; Alina Sikar-gang; Paul Lambotte; Javan Esfandiari; Gregory C. Ireton; Aarthy Vallur; Steven G. Reed; Gareth Jones; H. Martin Vordermeier; Judy R. Stabel; Tyler C. Thacker; Mitchell V. Palmer; W. Ray Waters; Patricia P. Wilkins

doi:10.1128/CVI.00259-17

Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis

Konstantin P. Lyashchenko, Adrian Grandison, Karen Keskinen, Alina Sikar-gang, Paul Lambotte, Javan Esfandiari, Gregory C. Ireton, Aarthy Vallur, Steven G. Reed, Gareth Jones, H. Martin Vordermeier, Judy R. Stabel, Tyler C. Thacker, Mitchell V. Palmer, W. Ray Waters, Patricia P. Wilkins (Editor)

Bovine TB, diseases and control

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Bovine tuberculosis (TB), caused by Mycobacterium bovis, remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we screened a panel of 101 recombinant proteins, including 10 polyepitope fusions, by a multiantigen print immunoassay (MAPIA) with well-characterized serum samples serially collected from cattle with experimental or naturally acquired M. bovis infection. A novel set of 12 seroreactive antigens was established. Evaluation of selected proteins in the dual-path platform (DPP) assay showed that the highest diagnostic accuracy (∼95%) was achieved with a cocktail of five best-performing antigens, thus demonstrating the potential for development of an improved and more practical serodiagnostic test for bovine TB.

Original language	English
Article number	e00259-17
Number of pages	9
Journal	Clinical and Vaccine Immunology
Volume	24
Issue number	12
DOIs	https://doi.org/10.1128/CVI.00259-17
Publication status	Published - 05 Dec 2017

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1128/CVI.00259-17

https://cvi.asm.org/content/24/12/e00259-17

Permanent link

Persistent link

Cite this

Lyashchenko, K. P., Grandison, A., Keskinen, K., Sikar-gang, A., Lambotte, P., Esfandiari, J., Ireton, G. C., Vallur, A., Reed, S. G., Jones, G., Vordermeier, H. M., Stabel, J. R., Thacker, T. C., Palmer, M. V., Waters, W. R., & Wilkins, P. P. (Ed.) (2017). Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis. Clinical and Vaccine Immunology, 24(12), Article e00259-17. https://doi.org/10.1128/CVI.00259-17

@article{a85de33464a240b49bcf8aeeb9407dc8,

title = "Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis",

abstract = "Bovine tuberculosis (TB), caused by Mycobacterium bovis, remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we screened a panel of 101 recombinant proteins, including 10 polyepitope fusions, by a multiantigen print immunoassay (MAPIA) with well-characterized serum samples serially collected from cattle with experimental or naturally acquired M. bovis infection. A novel set of 12 seroreactive antigens was established. Evaluation of selected proteins in the dual-path platform (DPP) assay showed that the highest diagnostic accuracy (∼95%) was achieved with a cocktail of five best-performing antigens, thus demonstrating the potential for development of an improved and more practical serodiagnostic test for bovine TB.",

author = "Lyashchenko, {Konstantin P.} and Adrian Grandison and Karen Keskinen and Alina Sikar-gang and Paul Lambotte and Javan Esfandiari and Ireton, {Gregory C.} and Aarthy Vallur and Reed, {Steven G.} and Gareth Jones and Vordermeier, {H. Martin} and Stabel, {Judy R.} and Thacker, {Tyler C.} and Palmer, {Mitchell V.} and Waters, {W. Ray} and Wilkins, {Patricia P.}",

year = "2017",

month = dec,

day = "5",

doi = "10.1128/CVI.00259-17",

language = "English",

volume = "24",

journal = "Clinical and Vaccine Immunology",

issn = "1556-6811",

publisher = "American Society for Microbiology",

number = "12",

}

Lyashchenko, KP, Grandison, A, Keskinen, K, Sikar-gang, A, Lambotte, P, Esfandiari, J, Ireton, GC, Vallur, A, Reed, SG, Jones, G, Vordermeier, HM, Stabel, JR, Thacker, TC, Palmer, MV, Waters, WR & Wilkins, PP (ed.) 2017, 'Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis', Clinical and Vaccine Immunology, vol. 24, no. 12, e00259-17. https://doi.org/10.1128/CVI.00259-17

TY - JOUR

T1 - Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis

AU - Lyashchenko, Konstantin P.

AU - Grandison, Adrian

AU - Keskinen, Karen

AU - Sikar-gang, Alina

AU - Lambotte, Paul

AU - Esfandiari, Javan

AU - Ireton, Gregory C.

AU - Vallur, Aarthy

AU - Reed, Steven G.

AU - Jones, Gareth

AU - Vordermeier, H. Martin

AU - Stabel, Judy R.

AU - Thacker, Tyler C.

AU - Palmer, Mitchell V.

AU - Waters, W. Ray

A2 - Wilkins, Patricia P.

PY - 2017/12/5

Y1 - 2017/12/5

N2 - Bovine tuberculosis (TB), caused by Mycobacterium bovis, remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we screened a panel of 101 recombinant proteins, including 10 polyepitope fusions, by a multiantigen print immunoassay (MAPIA) with well-characterized serum samples serially collected from cattle with experimental or naturally acquired M. bovis infection. A novel set of 12 seroreactive antigens was established. Evaluation of selected proteins in the dual-path platform (DPP) assay showed that the highest diagnostic accuracy (∼95%) was achieved with a cocktail of five best-performing antigens, thus demonstrating the potential for development of an improved and more practical serodiagnostic test for bovine TB.

AB - Bovine tuberculosis (TB), caused by Mycobacterium bovis, remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we screened a panel of 101 recombinant proteins, including 10 polyepitope fusions, by a multiantigen print immunoassay (MAPIA) with well-characterized serum samples serially collected from cattle with experimental or naturally acquired M. bovis infection. A novel set of 12 seroreactive antigens was established. Evaluation of selected proteins in the dual-path platform (DPP) assay showed that the highest diagnostic accuracy (∼95%) was achieved with a cocktail of five best-performing antigens, thus demonstrating the potential for development of an improved and more practical serodiagnostic test for bovine TB.

U2 - 10.1128/CVI.00259-17

DO - 10.1128/CVI.00259-17

M3 - Article

C2 - 28978510

SN - 1556-6811

VL - 24

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

IS - 12

M1 - e00259-17

ER -

Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis

Abstract

UN SDGs

Access to Document

Permanent link

Fingerprint

Cite this