Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis

Konstantin P. Lyashchenko; Adrian Grandison; Karen Keskinen; Alina Sikar-gang; Paul Lambotte; Javan Esfandiari; Gregory C. Ireton; Aarthy Vallur; Steven G. Reed; Gareth Jones; H. Martin Vordermeier; Judy R. Stabel; Tyler C. Thacker; Mitchell V. Palmer; W. Ray Waters; Patricia P. Wilkins

doi:10.1128/CVI.00259-17

Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis

Konstantin P. Lyashchenko
, Adrian Grandison
, Karen Keskinen
, Alina Sikar-gang
, Paul Lambotte
, Javan Esfandiari
, Gregory C. Ireton
, Aarthy Vallur
, Steven G. Reed
, Gareth Jones
, H. Martin Vordermeier
, Judy R. Stabel
, Tyler C. Thacker
, Mitchell V. Palmer
, W. Ray Waters
, Patricia P. Wilkins (Editor)

Bovine TB, diseases and control

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Bovine tuberculosis (TB), caused by Mycobacterium bovis, remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we screened a panel of 101 recombinant proteins, including 10 polyepitope fusions, by a multiantigen print immunoassay (MAPIA) with well-characterized serum samples serially collected from cattle with experimental or naturally acquired M. bovis infection. A novel set of 12 seroreactive antigens was established. Evaluation of selected proteins in the dual-path platform (DPP) assay showed that the highest diagnostic accuracy (∼95%) was achieved with a cocktail of five best-performing antigens, thus demonstrating the potential for development of an improved and more practical serodiagnostic test for bovine TB.

Original language	English
Article number	e00259-17
Number of pages	9
Journal	Clinical and Vaccine Immunology
Volume	24
Issue number	12
DOIs	https://doi.org/10.1128/CVI.00259-17
Publication status	Published - 05 Dec 2017

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https://cvi.asm.org/content/24/12/e00259-17

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Lyashchenko, K. P., Grandison, A., Keskinen, K., Sikar-gang, A., Lambotte, P., Esfandiari, J., Ireton, G. C., Vallur, A., Reed, S. G., Jones, G., Vordermeier, H. M., Stabel, J. R., Thacker, T. C., Palmer, M. V., Waters, W. R., & Wilkins, P. P. (Ed.) (2017). Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis. Clinical and Vaccine Immunology, 24(12), Article e00259-17. https://doi.org/10.1128/CVI.00259-17

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title = "Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis",

abstract = "Bovine tuberculosis (TB), caused by Mycobacterium bovis, remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we screened a panel of 101 recombinant proteins, including 10 polyepitope fusions, by a multiantigen print immunoassay (MAPIA) with well-characterized serum samples serially collected from cattle with experimental or naturally acquired M. bovis infection. A novel set of 12 seroreactive antigens was established. Evaluation of selected proteins in the dual-path platform (DPP) assay showed that the highest diagnostic accuracy (∼95\%) was achieved with a cocktail of five best-performing antigens, thus demonstrating the potential for development of an improved and more practical serodiagnostic test for bovine TB.",

author = "Lyashchenko, \{Konstantin P.\} and Adrian Grandison and Karen Keskinen and Alina Sikar-gang and Paul Lambotte and Javan Esfandiari and Ireton, \{Gregory C.\} and Aarthy Vallur and Reed, \{Steven G.\} and Gareth Jones and Vordermeier, \{H. Martin\} and Stabel, \{Judy R.\} and Thacker, \{Tyler C.\} and Palmer, \{Mitchell V.\} and Waters, \{W. Ray\} and Wilkins, \{Patricia P.\}",

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doi = "10.1128/CVI.00259-17",

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Lyashchenko, KP, Grandison, A, Keskinen, K, Sikar-gang, A, Lambotte, P, Esfandiari, J, Ireton, GC, Vallur, A, Reed, SG, Jones, G, Vordermeier, HM, Stabel, JR, Thacker, TC, Palmer, MV, Waters, WR & Wilkins, PP (ed.) 2017, 'Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis', Clinical and Vaccine Immunology, vol. 24, no. 12, e00259-17. https://doi.org/10.1128/CVI.00259-17

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T1 - Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis

AU - Lyashchenko, Konstantin P.

AU - Grandison, Adrian

AU - Keskinen, Karen

AU - Sikar-gang, Alina

AU - Lambotte, Paul

AU - Esfandiari, Javan

AU - Ireton, Gregory C.

AU - Vallur, Aarthy

AU - Reed, Steven G.

AU - Jones, Gareth

AU - Vordermeier, H. Martin

AU - Stabel, Judy R.

AU - Thacker, Tyler C.

AU - Palmer, Mitchell V.

AU - Waters, W. Ray

A2 - Wilkins, Patricia P.

PY - 2017/12/5

Y1 - 2017/12/5

N2 - Bovine tuberculosis (TB), caused by Mycobacterium bovis, remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we screened a panel of 101 recombinant proteins, including 10 polyepitope fusions, by a multiantigen print immunoassay (MAPIA) with well-characterized serum samples serially collected from cattle with experimental or naturally acquired M. bovis infection. A novel set of 12 seroreactive antigens was established. Evaluation of selected proteins in the dual-path platform (DPP) assay showed that the highest diagnostic accuracy (∼95%) was achieved with a cocktail of five best-performing antigens, thus demonstrating the potential for development of an improved and more practical serodiagnostic test for bovine TB.

AB - Bovine tuberculosis (TB), caused by Mycobacterium bovis, remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we screened a panel of 101 recombinant proteins, including 10 polyepitope fusions, by a multiantigen print immunoassay (MAPIA) with well-characterized serum samples serially collected from cattle with experimental or naturally acquired M. bovis infection. A novel set of 12 seroreactive antigens was established. Evaluation of selected proteins in the dual-path platform (DPP) assay showed that the highest diagnostic accuracy (∼95%) was achieved with a cocktail of five best-performing antigens, thus demonstrating the potential for development of an improved and more practical serodiagnostic test for bovine TB.

U2 - 10.1128/CVI.00259-17

DO - 10.1128/CVI.00259-17

M3 - Article

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SN - 1556-6811

VL - 24

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

IS - 12

M1 - e00259-17

ER -

Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis

Abstract

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