Identification of putative methylotrophic and hydrogenotrophic methanogens within sedimenting material and copepod faecal pellets

Arlene K. Ditchfield, Samuel T. Wilson, Mark C. Hart, Kevin J. Purdy, David H. Green, Angela D. Hatton*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

In the present study, sedimenting particulate material and faecal pellets from natural and laboratory-reared copepods were examined for the presence of methanogenic Archaea. 16S rRNA gene sequences closely related to Methanogenium, Methanobacterium and Methanolobus spp. were detected, indicating the potential for different methanogenic metabolisms (hydro geno - trophic and methylotrophic methanogenesis) in these samples. The present study represents the first 16S rRNA gene-based identification of methanogens in copepod faecal pellets and importantly the first reported 16S rRNA-based occurrence of potentially hydrogenotrophic methano - gens from marine particulate material, where it was previously thought that methylotrophic methano gens dominated methanogenesis. Anaerobic incubation experiments using sedimenting particulate material and copepod faecal pellets demonstrated active methane production and indicated that the associated methanogenic community was viable under the conditions tested.

Original languageEnglish
Pages (from-to)151-160
Number of pages10
JournalAquatic Microbial Ecology
Volume67
Issue number2
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • Copepod
  • Faecal pellets
  • Hydrogenotrophic methanogens
  • Methane production
  • Methyltrophic methanogens
  • Sedimenting particulate material

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