Projects per year
Abstract
Pea (Pisum sativum) is one of relatively few genetically amenable plant species with compound leaves. Pea leaves have a variety of specialized organs: leaflets, tendrils, pulvini and stipules, which enable the identification of mutations that transform or affect distinct parts of the leaf. Characterization of these mutations offers insights into the development and evolution of novel leaf traits. The previously characterized morphological gene Cochleata, conferring stipule identity, was known to interact with Stipules reduced (St), which conditions stipule size in pea, but the St gene remained unknown.
Here we analysed Fast Neutron irradiated pea mutants by restriction site associated DNA sequencing.
We identified St as a gene encoding a C2H2 zinc finger transcription factor that is regulated by Cochleata. St regulates both cell division and cell expansion in the stipule.
Our approach shows how systematic genome‐wide screens can be used successfully for the analysis of traits in species for which whole genome sequences are not available.
Here we analysed Fast Neutron irradiated pea mutants by restriction site associated DNA sequencing.
We identified St as a gene encoding a C2H2 zinc finger transcription factor that is regulated by Cochleata. St regulates both cell division and cell expansion in the stipule.
Our approach shows how systematic genome‐wide screens can be used successfully for the analysis of traits in species for which whole genome sequences are not available.
Original language | English |
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Pages (from-to) | 288-299 |
Number of pages | 11 |
Journal | New Phytologist |
Volume | 220 |
Issue number | 1 |
Early online date | 05 Jul 2018 |
DOIs | |
Publication status | Published - 01 Oct 2018 |
Keywords
- pea (Pisum sativum)
- leaf
- stipule
- RAD sequencing
- fast neutron
- mutant
- C2H2 zinc finger
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Dive into the research topics of 'Identification of Stipules reduced, a leaf morphology gene in pea (Pisum sativum)'. Together they form a unique fingerprint.Profiles
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Matthew Hegarty
- Faculty of Earth and Life Sciences, Department of Life Sciences - Senior Lecturer
Person: Teaching And Research
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Martin Swain
- Faculty of Earth and Life Sciences, Department of Life Sciences - Senior Lecturer
Person: Teaching And Research
Projects
- 1 Finished
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Bioinformatics and genomic and phenomic platform development
Armstead, I., Boyle, R., Doonan, J., Fernandez Fuentes, N., Gay, A., Hegarty, M., Huang, L., Neal, M., Swain, M. & Thomas, I.
Biotechnology and Biological Sciences Research Council
01 Apr 2012 → 31 Mar 2017
Project: Externally funded research