Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica released by Nonidet P40

R. M. Morphew; Clare M. Hamilton; H. A. Wright; David J. Dowling; Sandra M. O'Neill; P. M. Brophy

doi:10.1016/j.vetpar.2012.08.029

Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica released by Nonidet P40

R. M. Morphew, Clare M. Hamilton, H. A. Wright, David J. Dowling, Sandra M. O'Neill, P. M. Brophy

Department of Life Sciences

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Abstract

Fasciola hepatica NP-40 released protein extract (FhNPE) exhibits potent Th1 immunosuppressive properties in vitro and in vivo. However, the protein composition of this active fraction, responsible for Th1 immune modulatory activity, has yet to be resolved. Therefore, FhNPE, a Nonidet P-40 extract, was subjected to a proteomic analysis in order to identify individual protein components. This was performed using an in house F. hepatica EST database following 2D electrophoresis combined with de novo sequencing based mass spectrometry. The identified proteins, a mixture of excretory/secretory and membrane-associated proteins, are associated with stress response and chaperoning, energy metabolism and cytoskeletal components. The immune modulatory properties of these identified protein(s) are discussed and HSP70 from F. hepatica is highlighted as a potential host immune modulator for future study.

Original language	English
Pages (from-to)	379–385
Number of pages	7
Journal	Veterinary Parasitology
Volume	191
Issue number	3-4
Early online date	11 Sept 2012
DOIs	https://doi.org/10.1016/j.vetpar.2012.08.029
Publication status	Published - 31 Jan 2013

Keywords

Anti-inflammatory
Heat shock protein
Proteomics
Amino Acid Sequence
Molecular Sequence Data
Electrophoresis, Gel, Two-Dimensional
Fasciola hepatica/chemistry
Immunologic Factors/chemistry
Sequence Alignment
Animals
Mass Spectrometry
Polyethylene Glycols/chemistry
Octoxynol
Helminth Proteins/chemistry

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120712 Morphew et alAccepted author manuscript, 153 KB
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title = "Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica released by Nonidet P40",

abstract = "Fasciola hepatica NP-40 released protein extract (FhNPE) exhibits potent Th1 immunosuppressive properties in vitro and in vivo. However, the protein composition of this active fraction, responsible for Th1 immune modulatory activity, has yet to be resolved. Therefore, FhNPE, a Nonidet P-40 extract, was subjected to a proteomic analysis in order to identify individual protein components. This was performed using an in house F. hepatica EST database following 2D electrophoresis combined with de novo sequencing based mass spectrometry. The identified proteins, a mixture of excretory/secretory and membrane-associated proteins, are associated with stress response and chaperoning, energy metabolism and cytoskeletal components. The immune modulatory properties of these identified protein(s) are discussed and HSP70 from F. hepatica is highlighted as a potential host immune modulator for future study.",

keywords = "Anti-inflammatory, Heat shock protein, Proteomics, Amino Acid Sequence, Molecular Sequence Data, Electrophoresis, Gel, Two-Dimensional, Fasciola hepatica/chemistry, Immunologic Factors/chemistry, Sequence Alignment, Animals, Mass Spectrometry, Polyethylene Glycols/chemistry, Octoxynol, Helminth Proteins/chemistry",

author = "Morphew, {R. M.} and Hamilton, {Clare M.} and Wright, {H. A.} and Dowling, {David J.} and O'Neill, {Sandra M.} and Brophy, {P. M.}",

note = "Funding Information: The authors would like to thank Mr. Jim Heald and Dr. Charly Potter for their MS skills. The authors acknowledge the support of the BBSRC UK ( BBH0092561 ) and the Wellcome Trust (Grant WT094907MA ).",

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doi = "10.1016/j.vetpar.2012.08.029",

language = "English",

volume = "191",

pages = "379–385",

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AU - Hamilton, Clare M.

AU - Wright, H. A.

AU - Dowling, David J.

AU - O'Neill, Sandra M.

AU - Brophy, P. M.

N1 - Funding Information: The authors would like to thank Mr. Jim Heald and Dr. Charly Potter for their MS skills. The authors acknowledge the support of the BBSRC UK ( BBH0092561 ) and the Wellcome Trust (Grant WT094907MA ).

PY - 2013/1/31

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N2 - Fasciola hepatica NP-40 released protein extract (FhNPE) exhibits potent Th1 immunosuppressive properties in vitro and in vivo. However, the protein composition of this active fraction, responsible for Th1 immune modulatory activity, has yet to be resolved. Therefore, FhNPE, a Nonidet P-40 extract, was subjected to a proteomic analysis in order to identify individual protein components. This was performed using an in house F. hepatica EST database following 2D electrophoresis combined with de novo sequencing based mass spectrometry. The identified proteins, a mixture of excretory/secretory and membrane-associated proteins, are associated with stress response and chaperoning, energy metabolism and cytoskeletal components. The immune modulatory properties of these identified protein(s) are discussed and HSP70 from F. hepatica is highlighted as a potential host immune modulator for future study.

AB - Fasciola hepatica NP-40 released protein extract (FhNPE) exhibits potent Th1 immunosuppressive properties in vitro and in vivo. However, the protein composition of this active fraction, responsible for Th1 immune modulatory activity, has yet to be resolved. Therefore, FhNPE, a Nonidet P-40 extract, was subjected to a proteomic analysis in order to identify individual protein components. This was performed using an in house F. hepatica EST database following 2D electrophoresis combined with de novo sequencing based mass spectrometry. The identified proteins, a mixture of excretory/secretory and membrane-associated proteins, are associated with stress response and chaperoning, energy metabolism and cytoskeletal components. The immune modulatory properties of these identified protein(s) are discussed and HSP70 from F. hepatica is highlighted as a potential host immune modulator for future study.

KW - Anti-inflammatory

KW - Heat shock protein

KW - Proteomics

KW - Amino Acid Sequence

KW - Molecular Sequence Data

KW - Electrophoresis, Gel, Two-Dimensional

KW - Fasciola hepatica/chemistry

KW - Immunologic Factors/chemistry

KW - Sequence Alignment

KW - Animals

KW - Mass Spectrometry

KW - Polyethylene Glycols/chemistry

KW - Octoxynol

KW - Helminth Proteins/chemistry

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M3 - Article

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VL - 191

SP - 379

EP - 385

JO - Veterinary Parasitology

JF - Veterinary Parasitology

IS - 3-4

ER -

Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica released by Nonidet P40

Abstract

Keywords

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Developing a 'Validation Portfolio' to Exploit Key Virulence Proteins in Fasciola Species for Parasite Control

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Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica released by Nonidet P40

Abstract

Keywords

Access to Document

Permanent link

Other files and links

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Projects

Developing a 'Validation Portfolio' to Exploit Key Virulence Proteins in Fasciola Species for Parasite Control

Cite this