Iminosugar Amino Acid idoBR1 Reduces Inflammatory Responses in Microglia

OA Olajide; VU Iwuanyanwu; OW Banjo; A Kato; YB Penkova; GWJ Fleet; RJ Nash

doi:10.3390/molecules27103342

Iminosugar Amino Acid idoBR1 Reduces Inflammatory Responses in Microglia

OA Olajide, VU Iwuanyanwu, OW Banjo, A Kato, YB Penkova, GWJ Fleet, RJ Nash

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Abstract

Background
Inflammation is reported to be a key factor in neurodegeneration. The microglia are immune cells present in the central nervous system; their activation results in the release of inflammatory cytokines and is thought to be related to aging and neurodegenerative disorders, such as Alzheimer’s disease.

Methods
A mouse BV-2 microglia cell line was activated using LPS and the anti-inflammatory cucumber-derived iminosugar amino acid idoBR1, (2R,3R,4R,5S)-3,4,5-trihydroxypiperidine-2-carboxylic acid, was used alongside dexamethasone as the control to determine whether it could reduce the inflammatory responses.

Results
A dose-dependent reduction in the LPS-induced production of the proinflammatory factors TNFα, IL-6, and nitric oxide and the transcription factor NF-κB was found.

Conclusions
Further investigations of the anti-inflammatory effects of idoBR1 in other models of neurodegenerative diseases are warranted.

Original language	English
Article number	3342
Number of pages	11
Journal	Molecules
Volume	27
Issue number	10
DOIs	https://doi.org/10.3390/molecules27103342
Publication status	Published - 23 May 2022
Externally published	Yes

Keywords

(2R,3R,4R,5S)-3,4,5-trihydroxypiperidine-2-carboxylic acid
Cucumis sativus
iminosugar amino acid-idoBR1
lipopolysaccharide
microglia cell line
neuroprotection
pro-inflammatory factors

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ArticleFinal published version, 1.47 MBLicence: CC BY

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@article{d7357625ffa446c58c22b4fef39e7cca,

title = "Iminosugar Amino Acid idoBR1 Reduces Inflammatory Responses in Microglia",

abstract = "BackgroundInflammation is reported to be a key factor in neurodegeneration. The microglia are immune cells present in the central nervous system; their activation results in the release of inflammatory cytokines and is thought to be related to aging and neurodegenerative disorders, such as Alzheimer{\textquoteright}s disease.MethodsA mouse BV-2 microglia cell line was activated using LPS and the anti-inflammatory cucumber-derived iminosugar amino acid idoBR1, (2R,3R,4R,5S)-3,4,5-trihydroxypiperidine-2-carboxylic acid, was used alongside dexamethasone as the control to determine whether it could reduce the inflammatory responses.ResultsA dose-dependent reduction in the LPS-induced production of the proinflammatory factors TNFα, IL-6, and nitric oxide and the transcription factor NF-κB was found.ConclusionsFurther investigations of the anti-inflammatory effects of idoBR1 in other models of neurodegenerative diseases are warranted.",

keywords = "(2R,3R,4R,5S)-3,4,5-trihydroxypiperidine-2-carboxylic acid, Cucumis sativus, iminosugar amino acid-idoBR1, lipopolysaccharide, microglia cell line, neuroprotection, pro-inflammatory factors",

author = "OA Olajide and VU Iwuanyanwu and OW Banjo and A Kato and YB Penkova and GWJ Fleet and RJ Nash",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2022",

month = may,

day = "23",

doi = "10.3390/molecules27103342",

language = "English",

volume = "27",

journal = "Molecules",

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TY - JOUR

T1 - Iminosugar Amino Acid idoBR1 Reduces Inflammatory Responses in Microglia

AU - Olajide, OA

AU - Iwuanyanwu, VU

AU - Banjo, OW

AU - Kato, A

AU - Penkova, YB

AU - Fleet, GWJ

AU - Nash, RJ

PY - 2022/5/23

Y1 - 2022/5/23

N2 - BackgroundInflammation is reported to be a key factor in neurodegeneration. The microglia are immune cells present in the central nervous system; their activation results in the release of inflammatory cytokines and is thought to be related to aging and neurodegenerative disorders, such as Alzheimer’s disease.MethodsA mouse BV-2 microglia cell line was activated using LPS and the anti-inflammatory cucumber-derived iminosugar amino acid idoBR1, (2R,3R,4R,5S)-3,4,5-trihydroxypiperidine-2-carboxylic acid, was used alongside dexamethasone as the control to determine whether it could reduce the inflammatory responses.ResultsA dose-dependent reduction in the LPS-induced production of the proinflammatory factors TNFα, IL-6, and nitric oxide and the transcription factor NF-κB was found.ConclusionsFurther investigations of the anti-inflammatory effects of idoBR1 in other models of neurodegenerative diseases are warranted.

AB - BackgroundInflammation is reported to be a key factor in neurodegeneration. The microglia are immune cells present in the central nervous system; their activation results in the release of inflammatory cytokines and is thought to be related to aging and neurodegenerative disorders, such as Alzheimer’s disease.MethodsA mouse BV-2 microglia cell line was activated using LPS and the anti-inflammatory cucumber-derived iminosugar amino acid idoBR1, (2R,3R,4R,5S)-3,4,5-trihydroxypiperidine-2-carboxylic acid, was used alongside dexamethasone as the control to determine whether it could reduce the inflammatory responses.ResultsA dose-dependent reduction in the LPS-induced production of the proinflammatory factors TNFα, IL-6, and nitric oxide and the transcription factor NF-κB was found.ConclusionsFurther investigations of the anti-inflammatory effects of idoBR1 in other models of neurodegenerative diseases are warranted.

KW - (2R,3R,4R,5S)-3,4,5-trihydroxypiperidine-2-carboxylic acid

KW - Cucumis sativus

KW - iminosugar amino acid-idoBR1

KW - lipopolysaccharide

KW - microglia cell line

KW - neuroprotection

KW - pro-inflammatory factors

UR - http://www.scopus.com/inward/record.url?scp=85130553852&partnerID=8YFLogxK

U2 - 10.3390/molecules27103342

DO - 10.3390/molecules27103342

M3 - Article

C2 - 35630818

SN - 1420-3049

VL - 27

JO - Molecules

JF - Molecules

IS - 10

M1 - 3342

ER -

Iminosugar Amino Acid idoBR1 Reduces Inflammatory Responses in Microglia

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