Immune reactions of CD4- and CD8-positive T cell subpopulations in spleen and lymph nodes of guinea pigs after vaccination with Bacillus Calmette Guerin

Thomas Klünner, Thomas Bartels, Martin Vordermeier, Reinhard Burger, Hubert Schäfer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (SciVal)

Abstract

Vaccination of guinea pigs with Mycobacterium bovis BCG confers partial resistance against infection with Mycobacterium tuberculosis. Induction of immunity is associated with a strong T cell response. The reactions of the cytotoxic and helper T lymphocyte subsets after BCG vaccination were analyzed by cytofluorometry and in functional tests. The relative number of CD8+ T cells in the spleen increased substantially after injection of BCG. In vitro restimulation after immunization induced a strong proliferative response but no cytotoxic reactions of CD8+ T cells against BCG-infected macrophages. A specific induction of IFN-γ and RANTES mRNA was observed after vaccination particularly in CD8+ but not in CD4+ T cells of the lymph nodes.

Original languageEnglish
Pages (from-to)1968-1977
Number of pages10
JournalVaccine
Volume19
Issue number15-16
Early online date22 Feb 2001
DOIs
Publication statusPublished - 28 Feb 2001

Keywords

  • Guinea pig
  • Mycobacteria
  • T lymphocytes

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