Immune reactions of CD4- and CD8-positive T cell subpopulations in spleen and lymph nodes of guinea pigs after vaccination with Bacillus Calmette Guerin

Thomas Klünner; Thomas Bartels; Martin Vordermeier; Reinhard Burger; Hubert Schäfer

doi:10.1016/S0264-410X(00)00429-1

Immune reactions of CD4- and CD8-positive T cell subpopulations in spleen and lymph nodes of guinea pigs after vaccination with Bacillus Calmette Guerin

Thomas Klünner, Thomas Bartels, Martin Vordermeier, Reinhard Burger, Hubert Schäfer^*

^*Corresponding author for this work

Bovine TB, diseases and control

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Vaccination of guinea pigs with Mycobacterium bovis BCG confers partial resistance against infection with Mycobacterium tuberculosis. Induction of immunity is associated with a strong T cell response. The reactions of the cytotoxic and helper T lymphocyte subsets after BCG vaccination were analyzed by cytofluorometry and in functional tests. The relative number of CD8⁺ T cells in the spleen increased substantially after injection of BCG. In vitro restimulation after immunization induced a strong proliferative response but no cytotoxic reactions of CD8⁺ T cells against BCG-infected macrophages. A specific induction of IFN-γ and RANTES mRNA was observed after vaccination particularly in CD8⁺ but not in CD4⁺ T cells of the lymph nodes.

Original language	English
Pages (from-to)	1968-1977
Number of pages	10
Journal	Vaccine
Volume	19
Issue number	15-16
Early online date	22 Feb 2001
DOIs	https://doi.org/10.1016/S0264-410X(00)00429-1
Publication status	Published - 28 Feb 2001

Keywords

Guinea pig
Mycobacteria
T lymphocytes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/S0264-410X(00)00429-1

Permanent link

Persistent link

Cite this

@article{52bafb01380041fd96856bf94ce9c008,

title = "Immune reactions of CD4- and CD8-positive T cell subpopulations in spleen and lymph nodes of guinea pigs after vaccination with Bacillus Calmette Guerin",

abstract = "Vaccination of guinea pigs with Mycobacterium bovis BCG confers partial resistance against infection with Mycobacterium tuberculosis. Induction of immunity is associated with a strong T cell response. The reactions of the cytotoxic and helper T lymphocyte subsets after BCG vaccination were analyzed by cytofluorometry and in functional tests. The relative number of CD8+ T cells in the spleen increased substantially after injection of BCG. In vitro restimulation after immunization induced a strong proliferative response but no cytotoxic reactions of CD8+ T cells against BCG-infected macrophages. A specific induction of IFN-γ and RANTES mRNA was observed after vaccination particularly in CD8+ but not in CD4+ T cells of the lymph nodes.",

keywords = "Guinea pig, Mycobacteria, T lymphocytes",

author = "Thomas Kl{\"u}nner and Thomas Bartels and Martin Vordermeier and Reinhard Burger and Hubert Sch{\"a}fer",

year = "2001",

month = feb,

day = "28",

doi = "10.1016/S0264-410X(00)00429-1",

language = "English",

volume = "19",

pages = "1968--1977",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier",

number = "15-16",

}

TY - JOUR

T1 - Immune reactions of CD4- and CD8-positive T cell subpopulations in spleen and lymph nodes of guinea pigs after vaccination with Bacillus Calmette Guerin

AU - Klünner, Thomas

AU - Bartels, Thomas

AU - Vordermeier, Martin

AU - Burger, Reinhard

AU - Schäfer, Hubert

PY - 2001/2/28

Y1 - 2001/2/28

N2 - Vaccination of guinea pigs with Mycobacterium bovis BCG confers partial resistance against infection with Mycobacterium tuberculosis. Induction of immunity is associated with a strong T cell response. The reactions of the cytotoxic and helper T lymphocyte subsets after BCG vaccination were analyzed by cytofluorometry and in functional tests. The relative number of CD8+ T cells in the spleen increased substantially after injection of BCG. In vitro restimulation after immunization induced a strong proliferative response but no cytotoxic reactions of CD8+ T cells against BCG-infected macrophages. A specific induction of IFN-γ and RANTES mRNA was observed after vaccination particularly in CD8+ but not in CD4+ T cells of the lymph nodes.

AB - Vaccination of guinea pigs with Mycobacterium bovis BCG confers partial resistance against infection with Mycobacterium tuberculosis. Induction of immunity is associated with a strong T cell response. The reactions of the cytotoxic and helper T lymphocyte subsets after BCG vaccination were analyzed by cytofluorometry and in functional tests. The relative number of CD8+ T cells in the spleen increased substantially after injection of BCG. In vitro restimulation after immunization induced a strong proliferative response but no cytotoxic reactions of CD8+ T cells against BCG-infected macrophages. A specific induction of IFN-γ and RANTES mRNA was observed after vaccination particularly in CD8+ but not in CD4+ T cells of the lymph nodes.

KW - Guinea pig

KW - Mycobacteria

KW - T lymphocytes

UR - https://www.scopus.com/pages/publications/0035961551

U2 - 10.1016/S0264-410X(00)00429-1

DO - 10.1016/S0264-410X(00)00429-1

M3 - Article

C2 - 11228367

AN - SCOPUS:0035961551

SN - 0264-410X

VL - 19

SP - 1968

EP - 1977

JO - Vaccine

JF - Vaccine

IS - 15-16

ER -

Immune reactions of CD4- and CD8-positive T cell subpopulations in spleen and lymph nodes of guinea pigs after vaccination with Bacillus Calmette Guerin

Abstract

Keywords

UN SDGs

Access to Document

Permanent link

Other files and links

Fingerprint

Cite this