Immunogenicity and protection against mycobacterium caprae challenge in goats vaccinated with BCG and revaccinated after one year

Claudia Arrieta-Villegas*, Enric Vidal, Maite Martín, Judit Verdés, Xavier Moll, Yvonne Espada, Mahavir Singh, Bernardo Villarreal-Ramos, Mariano Domingo, Bernat Pérez de Val

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Vaccination has been proposed as a supplementary tool for the control of tuberculosis in livestock. The long-term immunogenicity elicited by bacillus Calmette–Guerin (BCG) and the efficacy of revaccination were investigated in thirty goat kids distributed into three groups: unvaccinated controls, BCG (vaccinated at week 0) and BCG-BCG (vaccinated at weeks 0 and 56). Sixty-four weeks after the first vaccination, all animals were challenged with Mycobacterium caprae and examined post-mortem (pathology and bacterial load) at week 73. Antigen-specific interferon-gamma (IFN-γ) release was measured throughout the experiment. At week 59, peripheral blood mononuclear cells were stained for CD4, CD45RO and IFN-γ to determine the presence of antigen-specific cells secreting IFN-γ. The BCG-BCG group showed reductions in rectal temperatures, M. caprae DNA load in pulmonary lymph nodes (LN), the volume of lesions in pulmonary LN, mineralization in lungs, and higher weight gains compared to unvaccinated controls. IFN-γ responses were undetectable from 32 weeks after primary vaccination until revaccination, when the BCG-BCG group showed detectable IFN-γ production and a greater percentage of antigen-specific CD4+CD45RO+IFNγ+ and CD4CD45RO+IFNγ+ cells compared to the BCG and control groups, which may be an indicator of the mechanisms of protection. Thus, re-vaccination of goats with BCG appears to prolong protection against infection with M. caprae.

Original languageEnglish
Article number751
Number of pages16
Issue number4
Publication statusPublished - 10 Dec 2020


  • Antigen-specific memory T-cells
  • BCG
  • Diagnosis
  • Duration of immunity
  • Goat
  • Interferon gamma
  • Revaccination
  • Tuberculosis
  • Vaccine


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