In silico approaches illustrate the evolutionary pattern and protein-small molecule interactions of quinolone synthase from Aegle marmelos Correa

V Mallika, K C Sivakumar, G Aiswarya, E V Soniya

Research output: Contribution to journalArticlepeer-review

Abstract

Quinolone synthase from Aegle marmelos (AmQNS) is a Rutacean-specific plant type III polyketide synthase that synthesizes quinolone, acridone, and benzalacetone with therapeutic potential. Simple architecture and broad substrate affinity of AmQNS make it as one of the target enzymes to produce novel structural scaffolds. Another unique feature of AmQNS despite its high similarity to acridone forming type III polyketide synthase from Citrus microcarpa is the variation in the product formation. Hence, to explore the characteristic features of AmQNS, an in-depth sequence and structure-based bioinformatics analyses were performed. Our studies indicated that AmQNS and its nearest homologs have evolved by a series of gene duplication events and strong purifying selection pressure constrains them in the evolutionary process. Additionally, some amino acid alterations were identified in the functionally important region(s), which can contribute to the functional divergence of the enzyme. Prediction of favorable amino acid substitutions will be advantageous in the metabolic engineering of AmQNS for the production of novel compounds. Furthermore, comparative modeling and docking studies were utilized to investigate the structural behavior and small molecule interaction pattern of AmQNS. The observations and results reported here are crucial for advancing our understanding of AmQNS's phylogenetic position, selection pressure, evolvability, interaction pattern and thus providing the foundation for further studies on the structural and reaction mechanism.

Original languageEnglish
Pages (from-to)195-209
Number of pages15
JournalJournal of Biomolecular Structure & Dynamics
Volume37
Issue number1
Early online date24 Jan 2018
DOIs
Publication statusPublished - 02 Jan 2019
Externally publishedYes

Keywords

  • Aegle/chemistry
  • Amino Acid Substitution
  • Biological Evolution
  • Drug Design
  • Ligands
  • Models, Molecular
  • Molecular Docking Simulation
  • Molecular Dynamics Simulation
  • Molecular Structure
  • Mutation
  • Phylogeny
  • Polyketide Synthases/chemistry
  • Protein Binding
  • Quantitative Structure-Activity Relationship
  • Selection, Genetic
  • evolutionary analysis
  • Aegle marmelos
  • AmQNS
  • type III PKS
  • purifying selection
  • quinolone synthase

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