In vivo measurement of lipogenesis in ruminants using [1-14C]acetate

H. M. R. Greathead, J. M. Dawson, Nigel D. Scollan, P. J. Buttery

Research output: Contribution to journalArticlepeer-review

Abstract

A method for the measurement of the rate of lipogenesis in ruminants using a continuous intravenous infusion of [1-14C]acetate and measuring the rate of [1-14C]acetate incorporation into adipose tissue lipid was evaluated. Subcutaneous adipose tissue samples obtained by biopsy over the course of a 6 h continuous intravenous infusion of [1-14C]acetate into a wether and a steer maintained in a ‘metabolic steady state’ demonstrated that the incorporation of [1-14C]acetate into subcutaneous adipose tissue lipid was linear for the duration of the infusion period. Subsequent measures of rates of [1-14C]acetate incorporation into adipose tissue lipid were made on adipose tissue samples taken at a single time point during the infusion period. The technique was used to measure rates of lipogenesis in the subcutaneous adipose tissue of fourteen Hereford × Friesian steers that had been fed a pelleted diet of dried grass at a range of metabolizable energy (ME) intakes from 1·1 × ME requirement for maintenance to ad libitum for 11 weeks. Rates of lipogenesis increased linearly (P
Original languageEnglish
Pages (from-to)37-44
Number of pages8
JournalBritish Journal of Nutrition
Volume86
Issue number1
DOIs
Publication statusPublished - Jul 2001

Keywords

  • ruminant
  • lipogenesis
  • acetate
  • metabolizable energy intake

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