Inactivation of the Mycobacterium bovis homologue of the polymorphic RD1 gene Rv3879c (Mb3909c) does not affect virulence

Jacqueline Inwald; Keith Jahans; R. Glyn Hewinson; Stephen V. Gordon

doi:10.1016/j.tube.2003.08.018

Inactivation of the Mycobacterium bovis homologue of the polymorphic RD1 gene Rv3879c (Mb3909c) does not affect virulence

Jacqueline Inwald, Keith Jahans, R. Glyn Hewinson, Stephen V. Gordon^*

^*Corresponding author for this work

Bovine TB, diseases and control

Animal and Plant Health Agency

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The RD1 locus is deleted from all strains of Mycobacterium bovis BCG but present in virulent isolates of M. bovis and Mycobacterium tuberculosis. The RD1 gene Rv3879c encodes a proline- and alanine-rich protein that shows sequence polymorphism across members of the M. tuberculosis complex. The role of this protein in virulence was investigated by deleting the Rv3879c homologue from M. bovis (Mb3909c) and testing the virulence of the mutant in the guinea pig model. The M. bovis ΔMb3909c mutant was not attenuated in the guinea pig model, showing that this gene does not encode a virulence factor and plays no role in the attenuation caused by loss of RD1. Crown

Original language	English
Pages (from-to)	387-393
Number of pages	7
Journal	Tuberculosis
Volume	83
Issue number	6
DOIs	https://doi.org/10.1016/j.tube.2003.08.018
Publication status	Published - 13 Nov 2003

Keywords

Comparative genomics
Mycobacterium bovis
Virulence

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Inactivation of the Mycobacterium bovis homologue of the polymorphic RD1 gene Rv3879c (Mb3909c) does not affect virulence",

abstract = "The RD1 locus is deleted from all strains of Mycobacterium bovis BCG but present in virulent isolates of M. bovis and Mycobacterium tuberculosis. The RD1 gene Rv3879c encodes a proline- and alanine-rich protein that shows sequence polymorphism across members of the M. tuberculosis complex. The role of this protein in virulence was investigated by deleting the Rv3879c homologue from M. bovis (Mb3909c) and testing the virulence of the mutant in the guinea pig model. The M. bovis ΔMb3909c mutant was not attenuated in the guinea pig model, showing that this gene does not encode a virulence factor and plays no role in the attenuation caused by loss of RD1. Crown",

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AU - Inwald, Jacqueline

AU - Jahans, Keith

AU - Hewinson, R. Glyn

AU - Gordon, Stephen V.

PY - 2003/11/13

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N2 - The RD1 locus is deleted from all strains of Mycobacterium bovis BCG but present in virulent isolates of M. bovis and Mycobacterium tuberculosis. The RD1 gene Rv3879c encodes a proline- and alanine-rich protein that shows sequence polymorphism across members of the M. tuberculosis complex. The role of this protein in virulence was investigated by deleting the Rv3879c homologue from M. bovis (Mb3909c) and testing the virulence of the mutant in the guinea pig model. The M. bovis ΔMb3909c mutant was not attenuated in the guinea pig model, showing that this gene does not encode a virulence factor and plays no role in the attenuation caused by loss of RD1. Crown

AB - The RD1 locus is deleted from all strains of Mycobacterium bovis BCG but present in virulent isolates of M. bovis and Mycobacterium tuberculosis. The RD1 gene Rv3879c encodes a proline- and alanine-rich protein that shows sequence polymorphism across members of the M. tuberculosis complex. The role of this protein in virulence was investigated by deleting the Rv3879c homologue from M. bovis (Mb3909c) and testing the virulence of the mutant in the guinea pig model. The M. bovis ΔMb3909c mutant was not attenuated in the guinea pig model, showing that this gene does not encode a virulence factor and plays no role in the attenuation caused by loss of RD1. Crown

KW - Comparative genomics

KW - Mycobacterium bovis

KW - Virulence

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M3 - Article

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AN - SCOPUS:0344873211

SN - 1472-9792

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JO - Tuberculosis

JF - Tuberculosis

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ER -

Inactivation of the Mycobacterium bovis homologue of the polymorphic RD1 gene Rv3879c (Mb3909c) does not affect virulence

Abstract

Keywords

UN SDGs

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