Inactivation of the Mycobacterium bovis homologue of the polymorphic RD1 gene Rv3879c (Mb3909c) does not affect virulence

Jacqueline Inwald, Keith Jahans, R. Glyn Hewinson, Stephen V. Gordon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (SciVal)

Abstract

The RD1 locus is deleted from all strains of Mycobacterium bovis BCG but present in virulent isolates of M. bovis and Mycobacterium tuberculosis. The RD1 gene Rv3879c encodes a proline- and alanine-rich protein that shows sequence polymorphism across members of the M. tuberculosis complex. The role of this protein in virulence was investigated by deleting the Rv3879c homologue from M. bovis (Mb3909c) and testing the virulence of the mutant in the guinea pig model. The M. bovis ΔMb3909c mutant was not attenuated in the guinea pig model, showing that this gene does not encode a virulence factor and plays no role in the attenuation caused by loss of RD1. Crown

Original languageEnglish
Pages (from-to)387-393
Number of pages7
JournalTuberculosis
Volume83
Issue number6
DOIs
Publication statusPublished - 13 Nov 2003

Keywords

  • Comparative genomics
  • Mycobacterium bovis
  • Virulence

Fingerprint

Dive into the research topics of 'Inactivation of the Mycobacterium bovis homologue of the polymorphic RD1 gene Rv3879c (Mb3909c) does not affect virulence'. Together they form a unique fingerprint.

Cite this