Induction of cellular immunity to a mycobacterial antigen adsorbed on lamellar particles of lactide polymers

N. Venkataprasad, A. G.A. Coombes*, M. Singh, M. Rohde, K. Wilkinson, F. Hudecz, S. S. Davis, H. M. Vordermeier

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

54 Citations (SciVal)

Abstract

Microspheres prepared from synthetic, biodegradable poly (L-lactide) [PLA] and copolymers of lactide and glycolide such as poly (DL lactide co- glycolide) [PLG] have been widely investigated for controlled delivery of encapsulated vaccine antigens. In this study we describe novel lamellar microparticles produced from PLA to which protein antigens can be adsorbed. These particles when administered to mice, induced strong Th1-type T cell responses to the adsorbed 38 kDa protein antigen from M. tuberculosis characterised by high levels of Interferon-gamma. In addition to proteins, we were also able to adsorb synthetic peptides resulting in specific T cell proliferation. Induction of strong cellular immunity together with the versatility of antigen adsorption to these particles should make such lamellae a useful tool to deliver protective antigens from intracellular pathogens.

Original languageEnglish
Pages (from-to)1814-1819
Number of pages6
JournalVaccine
Volume17
Issue number15-16
Early online date18 Mar 1999
DOIs
Publication statusPublished - 09 Apr 1999

Keywords

  • Delivery system
  • Lactide polymers
  • Resorbable lamellae
  • Tuberculosis
  • Polyesters/chemistry
  • Drug Carriers
  • Lipoproteins/chemistry
  • Microspheres
  • Antigens, Bacterial/chemistry
  • Interleukin-4/analysis
  • Female
  • Peptide Fragments/chemical synthesis
  • Polyglactin 910/chemistry
  • Immunization
  • Mycobacterium tuberculosis/immunology
  • Lymphocyte Activation
  • Mice, Inbred C57BL
  • Cells, Cultured
  • Crystallization
  • Interferon-gamma/analysis
  • Animals
  • Adsorption
  • DNA/biosynthesis
  • Mice
  • Th1 Cells/immunology

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