Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine- 2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms

Fabienne Floret; Lal C. Chaudhary; William C. Ellis; Suliman El Hassan; N. McKain; C. J. Newbold; R. J. Wallace

doi:10.1128/aem.65.7.3258-3260.1999

Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine- 2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms

Fabienne Floret
, Lal C. Chaudhary
, William C. Ellis
, Suliman El Hassan
, N. McKain
, C. J. Newbold
, R. J. Wallace

Institute of Biological, Environmental, and Rural Sciences

Instituto de Nutricion e Higiene de los Alimentos
Indian Veterinary Research Institute
Texas A&M University
University of Khartoum

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl] pyrrolidine-2-carboxylic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.

Original language	English
Pages (from-to)	3258-3260
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	65
Issue number	7
DOIs	https://doi.org/10.1128/aem.65.7.3258-3260.1999
Publication status	Published - Jul 1999

Keywords

SP-NOV
PEPTIDES
NITROGEN-METABOLISM
RUMEN BACTERIA
FLUID
SHEEP
BREAKDOWN
Endopeptidases/metabolism
Proteins/metabolism
Enzyme Inhibitors
Rumen/metabolism
Bacteria/drug effects
Clostridium/drug effects
Poaceae
Pyrrolidines/pharmacology
Biphenyl Compounds/pharmacology
Onium Compounds/pharmacology
Animals
Deamination
Azetidines/pharmacology
Sheep
Proline/analogs & derivatives
Animal Feed

Access to Document

10.1128/aem.65.7.3258-3260.1999

http://aem.asm.org/content/65/7/3258.abstract

Permanent link

Persistent link

Cite this

Floret, F., Chaudhary, L. C., Ellis, W. C., El Hassan, S., McKain, N., Newbold, C. J., & Wallace, R. J. (1999). Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine- 2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms. Applied and Environmental Microbiology, 65(7), 3258-3260. https://doi.org/10.1128/aem.65.7.3258-3260.1999

@article{579e619aef6c450fb42ed7b862dc8fbd,

title = "Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine- 2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms",

abstract = "The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl] pyrrolidine-2-carboxylic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.",

keywords = "SP-NOV, PEPTIDES, NITROGEN-METABOLISM, RUMEN BACTERIA, FLUID, SHEEP, BREAKDOWN, Endopeptidases/metabolism, Proteins/metabolism, Enzyme Inhibitors, Rumen/metabolism, Bacteria/drug effects, Clostridium/drug effects, Poaceae, Pyrrolidines/pharmacology, Biphenyl Compounds/pharmacology, Onium Compounds/pharmacology, Animals, Deamination, Azetidines/pharmacology, Sheep, Proline/analogs \& derivatives, Animal Feed",

author = "Fabienne Floret and Chaudhary, \{Lal C.\} and Ellis, \{William C.\} and \{El Hassan\}, Suliman and N. McKain and Newbold, \{C. J.\} and Wallace, \{R. J.\}",

note = "Floret, F., Chaudhary, L. C., Ellis, W. C., El Hassan, S., McKain, N., Newbold, C. J., Wallace, R. J. (1999). Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diazl-1-enyl]pyrrolidine-2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms. Applied and Environmental Microbiology, 65 (7), 3258-3260.",

year = "1999",

month = jul,

doi = "10.1128/aem.65.7.3258-3260.1999",

language = "English",

volume = "65",

pages = "3258--3260",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "7",

}

Floret, F, Chaudhary, LC, Ellis, WC, El Hassan, S, McKain, N, Newbold, CJ & Wallace, RJ 1999, 'Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine- 2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms', Applied and Environmental Microbiology, vol. 65, no. 7, pp. 3258-3260. https://doi.org/10.1128/aem.65.7.3258-3260.1999

Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine- 2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms. / Floret, Fabienne; Chaudhary, Lal C.; Ellis, William C. et al.
In: Applied and Environmental Microbiology, Vol. 65, No. 7, 07.1999, p. 3258-3260.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine- 2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms

AU - Floret, Fabienne

AU - Chaudhary, Lal C.

AU - Ellis, William C.

AU - El Hassan, Suliman

AU - McKain, N.

AU - Newbold, C. J.

AU - Wallace, R. J.

N1 - Floret, F., Chaudhary, L. C., Ellis, W. C., El Hassan, S., McKain, N., Newbold, C. J., Wallace, R. J. (1999). Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diazl-1-enyl]pyrrolidine-2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms. Applied and Environmental Microbiology, 65 (7), 3258-3260.

PY - 1999/7

Y1 - 1999/7

N2 - The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl] pyrrolidine-2-carboxylic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.

AB - The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl] pyrrolidine-2-carboxylic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.

KW - SP-NOV

KW - PEPTIDES

KW - NITROGEN-METABOLISM

KW - RUMEN BACTERIA

KW - FLUID

KW - SHEEP

KW - BREAKDOWN

KW - Endopeptidases/metabolism

KW - Proteins/metabolism

KW - Enzyme Inhibitors

KW - Rumen/metabolism

KW - Bacteria/drug effects

KW - Clostridium/drug effects

KW - Poaceae

KW - Pyrrolidines/pharmacology

KW - Biphenyl Compounds/pharmacology

KW - Onium Compounds/pharmacology

KW - Animals

KW - Deamination

KW - Azetidines/pharmacology

KW - Sheep

KW - Proline/analogs & derivatives

KW - Animal Feed

UR - https://www.scopus.com/pages/publications/0033014984

U2 - 10.1128/aem.65.7.3258-3260.1999

DO - 10.1128/aem.65.7.3258-3260.1999

M3 - Article

C2 - 10388733

SN - 0099-2240

VL - 65

SP - 3258

EP - 3260

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 7

ER -

Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine- 2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms

Abstract

Keywords

Access to Document

Permanent link

Other files and links

Fingerprint

Cite this