Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine- 2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms

Fabienne Floret; Lal C. Chaudhary; William C. Ellis; Suliman El Hassan; N. McKain; C. J. Newbold; R. J. Wallace

doi:10.1128/aem.65.7.3258-3260.1999

Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine- 2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms

Fabienne Floret, Lal C. Chaudhary, William C. Ellis, Suliman El Hassan, N. McKain, C. J. Newbold, R. J. Wallace

Institute of Biological, Environmental, and Rural Sciences

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl] pyrrolidine-2-carboxylic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.

Original language	English
Pages (from-to)	3258-3260
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	65
Issue number	7
DOIs	https://doi.org/10.1128/aem.65.7.3258-3260.1999
Publication status	Published - Jul 1999

Keywords

SP-NOV
PEPTIDES
NITROGEN-METABOLISM
RUMEN BACTERIA
FLUID
SHEEP
BREAKDOWN
Endopeptidases/metabolism
Proteins/metabolism
Enzyme Inhibitors
Rumen/metabolism
Bacteria/drug effects
Clostridium/drug effects
Poaceae
Pyrrolidines/pharmacology
Biphenyl Compounds/pharmacology
Onium Compounds/pharmacology
Animals
Deamination
Azetidines/pharmacology
Sheep
Proline/analogs & derivatives
Animal Feed

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http://aem.asm.org/content/65/7/3258.abstract

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Floret, F., Chaudhary, L. C., Ellis, W. C., El Hassan, S., McKain, N., Newbold, C. J., & Wallace, R. J. (1999). Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine- 2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms. Applied and Environmental Microbiology, 65(7), 3258-3260. https://doi.org/10.1128/aem.65.7.3258-3260.1999

@article{579e619aef6c450fb42ed7b862dc8fbd,

title = "Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine- 2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms",

abstract = "The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl] pyrrolidine-2-carboxylic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.",

keywords = "SP-NOV, PEPTIDES, NITROGEN-METABOLISM, RUMEN BACTERIA, FLUID, SHEEP, BREAKDOWN, Endopeptidases/metabolism, Proteins/metabolism, Enzyme Inhibitors, Rumen/metabolism, Bacteria/drug effects, Clostridium/drug effects, Poaceae, Pyrrolidines/pharmacology, Biphenyl Compounds/pharmacology, Onium Compounds/pharmacology, Animals, Deamination, Azetidines/pharmacology, Sheep, Proline/analogs \& derivatives, Animal Feed",

author = "Fabienne Floret and Chaudhary, \{Lal C.\} and Ellis, \{William C.\} and \{El Hassan\}, Suliman and N. McKain and Newbold, \{C. J.\} and Wallace, \{R. J.\}",

note = "Floret, F., Chaudhary, L. C., Ellis, W. C., El Hassan, S., McKain, N., Newbold, C. J., Wallace, R. J. (1999). Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diazl-1-enyl]pyrrolidine-2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms. Applied and Environmental Microbiology, 65 (7), 3258-3260.",

year = "1999",

month = jul,

doi = "10.1128/aem.65.7.3258-3260.1999",

language = "English",

volume = "65",

pages = "3258--3260",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "7",

}

Floret, F, Chaudhary, LC, Ellis, WC, El Hassan, S, McKain, N, Newbold, CJ & Wallace, RJ 1999, 'Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine- 2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms', Applied and Environmental Microbiology, vol. 65, no. 7, pp. 3258-3260. https://doi.org/10.1128/aem.65.7.3258-3260.1999

Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine- 2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms. / Floret, Fabienne; Chaudhary, Lal C.; Ellis, William C. et al.
In: Applied and Environmental Microbiology, Vol. 65, No. 7, 07.1999, p. 3258-3260.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine- 2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms

AU - Floret, Fabienne

AU - Chaudhary, Lal C.

AU - Ellis, William C.

AU - El Hassan, Suliman

AU - McKain, N.

AU - Newbold, C. J.

AU - Wallace, R. J.

N1 - Floret, F., Chaudhary, L. C., Ellis, W. C., El Hassan, S., McKain, N., Newbold, C. J., Wallace, R. J. (1999). Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diazl-1-enyl]pyrrolidine-2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms. Applied and Environmental Microbiology, 65 (7), 3258-3260.

PY - 1999/7

Y1 - 1999/7

N2 - The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl] pyrrolidine-2-carboxylic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.

AB - The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl] pyrrolidine-2-carboxylic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.

KW - SP-NOV

KW - PEPTIDES

KW - NITROGEN-METABOLISM

KW - RUMEN BACTERIA

KW - FLUID

KW - SHEEP

KW - BREAKDOWN

KW - Endopeptidases/metabolism

KW - Proteins/metabolism

KW - Enzyme Inhibitors

KW - Rumen/metabolism

KW - Bacteria/drug effects

KW - Clostridium/drug effects

KW - Poaceae

KW - Pyrrolidines/pharmacology

KW - Biphenyl Compounds/pharmacology

KW - Onium Compounds/pharmacology

KW - Animals

KW - Deamination

KW - Azetidines/pharmacology

KW - Sheep

KW - Proline/analogs & derivatives

KW - Animal Feed

UR - https://www.scopus.com/pages/publications/0033014984

U2 - 10.1128/aem.65.7.3258-3260.1999

DO - 10.1128/aem.65.7.3258-3260.1999

M3 - Article

C2 - 10388733

SN - 0099-2240

VL - 65

SP - 3258

EP - 3260

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 7

ER -

Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine- 2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms

Abstract

Keywords

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