Influence of nitrogen source on the fermentation of fibre from barley straw and sugarbeet pulp by ruminal micro-organisms in vitro

M. J. Ranilla; M. D. Carro; S López; C. J. Newbold; R. J. Wallace

doi:10.1079/BJN2001475

Influence of nitrogen source on the fermentation of fibre from barley straw and sugarbeet pulp by ruminal micro-organisms in vitro

M. J. Ranilla, M. D. Carro, S López, C. J. Newbold, R. J. Wallace

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Incubations were carried out with a batch culture system to study the effects of different N sources on the fermentation by ruminal micro-organisms from Merino sheep of two fibre substrates derived from feedstuffs that differed in their fermentation rate. The substrates were neutral-detergent fibre (NDF) from barley straw and sugarbeet pulp. N sources were ammonia (NH4Cl) and peptides (Trypticase). Three treatments were made by replacing ammonia-N with peptide-N at levels of 0 (AMMO), 33 (PEPLOW) and 66 % (PEPHIGH) of total N. There were no differences (P>0.05) between treatments in NDF degradation for both the barley straw and the sugarbeet pulp. Peptides increased (P0.05) by the presence of peptides compared with the AMMO treatment, but values were greater for the PEPHIGH compared with the PEPLOW N source, reaching statistical significance (P0.05) by N source. These results indicate that N forms other than ammonia are needed for maximal growth of fibre-digesting ruminal micro-organisms.

Original language	English
Pages (from-to)	717-724
Number of pages	8
Journal	British Journal of Nutrition
Volume	86
Issue number	6
DOIs	https://doi.org/10.1079/BJN2001475
Publication status	Published - Dec 2001

Keywords

liquid-associated micro-organisms
BACTERIA
AMINO-ACIDS
SHEEP RUMEN
solids-associated micro-organisms
nitrogen
CONTINUOUS-CULTURE
SOLID FRACTIONS
IN-VITRO
batch cultures
CHEMICAL-COMPOSITION
DE-NOVO SYNTHESIS
GROWTH
rumen microbial protein synthesis
RUMEN DIGESTA

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title = "Influence of nitrogen source on the fermentation of fibre from barley straw and sugarbeet pulp by ruminal micro-organisms in vitro",

abstract = "Incubations were carried out with a batch culture system to study the effects of different N sources on the fermentation by ruminal micro-organisms from Merino sheep of two fibre substrates derived from feedstuffs that differed in their fermentation rate. The substrates were neutral-detergent fibre (NDF) from barley straw and sugarbeet pulp. N sources were ammonia (NH4Cl) and peptides (Trypticase). Three treatments were made by replacing ammonia-N with peptide-N at levels of 0 (AMMO), 33 (PEPLOW) and 66 % (PEPHIGH) of total N. There were no differences (P>0.05) between treatments in NDF degradation for both the barley straw and the sugarbeet pulp. Peptides increased (P0.05) by the presence of peptides compared with the AMMO treatment, but values were greater for the PEPHIGH compared with the PEPLOW N source, reaching statistical significance (P0.05) by N source. These results indicate that N forms other than ammonia are needed for maximal growth of fibre-digesting ruminal micro-organisms.",

keywords = "liquid-associated micro-organisms, BACTERIA, AMINO-ACIDS, SHEEP RUMEN, solids-associated micro-organisms, nitrogen, CONTINUOUS-CULTURE, SOLID FRACTIONS, IN-VITRO, batch cultures, CHEMICAL-COMPOSITION, DE-NOVO SYNTHESIS, GROWTH, rumen microbial protein synthesis, RUMEN DIGESTA",

author = "Ranilla, {M. J.} and Carro, {M. D.} and S L{\'o}pez and Newbold, {C. J.} and Wallace, {R. J.}",

note = "Ranilla, M. J., Carro, M. D., L{\'o}pez, S., Newbold, C. J., Wallace, R. J. (2001). Influence of nitrogen source on the fermentation of fibre from barley straw and sugarbeet pulp by ruminal micro-organisms in vitro. British Journal of Nutrition, 86 (6), 717-724.",

year = "2001",

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doi = "10.1079/BJN2001475",

language = "English",

volume = "86",

pages = "717--724",

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T1 - Influence of nitrogen source on the fermentation of fibre from barley straw and sugarbeet pulp by ruminal micro-organisms in vitro

AU - Ranilla, M. J.

AU - Carro, M. D.

AU - López, S

AU - Newbold, C. J.

AU - Wallace, R. J.

N1 - Ranilla, M. J., Carro, M. D., López, S., Newbold, C. J., Wallace, R. J. (2001). Influence of nitrogen source on the fermentation of fibre from barley straw and sugarbeet pulp by ruminal micro-organisms in vitro. British Journal of Nutrition, 86 (6), 717-724.

PY - 2001/12

Y1 - 2001/12

N2 - Incubations were carried out with a batch culture system to study the effects of different N sources on the fermentation by ruminal micro-organisms from Merino sheep of two fibre substrates derived from feedstuffs that differed in their fermentation rate. The substrates were neutral-detergent fibre (NDF) from barley straw and sugarbeet pulp. N sources were ammonia (NH4Cl) and peptides (Trypticase). Three treatments were made by replacing ammonia-N with peptide-N at levels of 0 (AMMO), 33 (PEPLOW) and 66 % (PEPHIGH) of total N. There were no differences (P>0.05) between treatments in NDF degradation for both the barley straw and the sugarbeet pulp. Peptides increased (P0.05) by the presence of peptides compared with the AMMO treatment, but values were greater for the PEPHIGH compared with the PEPLOW N source, reaching statistical significance (P0.05) by N source. These results indicate that N forms other than ammonia are needed for maximal growth of fibre-digesting ruminal micro-organisms.

AB - Incubations were carried out with a batch culture system to study the effects of different N sources on the fermentation by ruminal micro-organisms from Merino sheep of two fibre substrates derived from feedstuffs that differed in their fermentation rate. The substrates were neutral-detergent fibre (NDF) from barley straw and sugarbeet pulp. N sources were ammonia (NH4Cl) and peptides (Trypticase). Three treatments were made by replacing ammonia-N with peptide-N at levels of 0 (AMMO), 33 (PEPLOW) and 66 % (PEPHIGH) of total N. There were no differences (P>0.05) between treatments in NDF degradation for both the barley straw and the sugarbeet pulp. Peptides increased (P0.05) by the presence of peptides compared with the AMMO treatment, but values were greater for the PEPHIGH compared with the PEPLOW N source, reaching statistical significance (P0.05) by N source. These results indicate that N forms other than ammonia are needed for maximal growth of fibre-digesting ruminal micro-organisms.

KW - liquid-associated micro-organisms

KW - BACTERIA

KW - AMINO-ACIDS

KW - SHEEP RUMEN

KW - solids-associated micro-organisms

KW - nitrogen

KW - CONTINUOUS-CULTURE

KW - SOLID FRACTIONS

KW - IN-VITRO

KW - batch cultures

KW - CHEMICAL-COMPOSITION

KW - DE-NOVO SYNTHESIS

KW - GROWTH

KW - rumen microbial protein synthesis

KW - RUMEN DIGESTA

UR - http://hdl.handle.net/2160/9396

U2 - 10.1079/BJN2001475

DO - 10.1079/BJN2001475

M3 - Article

SN - 0007-1145

VL - 86

SP - 717

EP - 724

JO - British Journal of Nutrition

JF - British Journal of Nutrition

IS - 6

ER -

Influence of nitrogen source on the fermentation of fibre from barley straw and sugarbeet pulp by ruminal micro-organisms in vitro

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