Inhibition of glutathione S-transferases (GSTs) from parasitic nematodes by extracts from traditional Nigerian medicinal plants

B. B. Fakae, A. M. Campbell, John Barrett, Ian Scott, P. H. Teesdale-Spittle, E. Liebau, Peter Brophy

Research output: Contribution to journalArticlepeer-review

49 Citations (SciVal)

Abstract

Piliostigma thonningii, Ocimum gratissimum, Nauclea latifolia and Alstonia boonei are used in Nigerian traditional medicines against gastrointestinal helminths of animals and man. Proanthocyanidins were detected in Piliostigma and Nauclea, but not Alstonia or Ocimum. Extracts of these plants killed 50% of brine shrimp nauplii at <10 ppm (Nauclea), 100 ppm (Piliostigma) and <1000 ppm (Ocimum and Alstonia), the Nauclea LD50 being similar to the anthelmintic drug piperazine. Extracts were also toxic to the parasitic nematode Haemonchus infective L3 stage. Nematode glutathione-S-transferases (GSTs) are potential drug targets. Apart from Alstonia all the medicinal plants contained heat-stable inhibitory activities against recombinant Ascaris and Onchocerca GSTs in vitro. Piliostigma, Ocimum and Nauclea had IC50s of 2, 10 and 15 µg/mL respectively for Ascaris GST and 4, 8, 28 µg/mL respectively for Onchocerca GST. We suggest that the inhibitory properties of some of these Nigerian plant extracts against GST may contribute to the pharmacological basis of their efficacy against helminths in traditional herbal use. Copyright © 2000 John Wiley & Sons, Ltd
Original languageEnglish
Pages (from-to)630-634
Number of pages5
JournalPhytotherapy Research
Volume630-634
Issue number5
Early online date29 Nov 2000
DOIs
Publication statusPublished - 01 Dec 2000

Keywords

  • nematode
  • Piliostigma thonningii
  • glutathine-S-transferase
  • proanthrocyanidin
  • anthelmintic

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