Inhibitory effects of Zingiber officinale roscoe derived components on aldose reductase activity in vitro and in vivo

Atsushi Kato, Yasuko Higuchi, Hirozo Goto, Haruhisa Kizu, Tadashi Okamoto, Naoki Asano, Jackie Hollinshead, Robert J. Nash, Isao Adachi

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63 Citations (SciVal)


Ginger (Zingiber officinale Roscoe) continues to be used as an important cooking spice and herbal medicine around the world. Scientific research has gradually verified the antidiabetic effects of ginger. Especially gingerols, which are the major components of ginger, are known to improve diabetes including the effect of enhancement against insulin-sensitivity. Aldose reductase inhibitors have considerable potential for the treatment of diabetes, without increased risk of hypoglycemia. The assay for aldose reductase inhibitors in ginger led to the isolation of five active compounds including 2-(4-hydroxy-3-methoxyphenyl)ethanol (2) and 2-(4-hydroxy-3-methoxyphenyl)ethanoic acid (3). Compounds 2 and 3 were good inhibitors of recombinant human aldose reductase, with IC50 values of 19.2 ± 1.9 and 18.5 ± 1.1 μM, respectively. Furthermore, these compounds significantly suppressed not only sorbitol accumulation in human erythrocytes but also lens galactitol accumulation in 30% of galactose-fed cataract rat model. A structure−activity relationship study revealed that the applicable side alkyl chain length and the presence of a C3 OCH3 group in the aromatic ring are essential features for enzyme recognition and binding. These results suggested that it would contribute to the protection against or improvement of diabetic complications for a dietary supplement of ginger or its extract containing aldose reductase inhibitors.
Original languageEnglish
Pages (from-to)6640-6644
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Issue number18
Early online date05 Aug 2006
Publication statusPublished - 06 Sept 2006


  • Zingiber officinale
  • ginger
  • aldose redudtase inhibitor
  • erythrocyte
  • sorbitol
  • galactosemia
  • diabetes


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