Interleukin-17A as a Biomarker for Bovine Tuberculosis

W. Ray Waters*, Mayara F. Maggioli, Mitchell V. Palmer, Tyler C. Thacker, Jodi L. McGill, H. Martin Vordermeier, Linda Berney-Meyer, William R. Jacobs, Michelle H. Larsen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Citations (SciVal)


T helper 17 (Th17)-associated cytokines are integral to the immune responses to tuberculosis, initiating both protective and harmful inflammatory responses. The aim of the present study was to evaluate applied aspects of interleukin-17 (IL-17) biology in the context of Mycobacterium bovis infection of cattle. Using transcriptome sequencing (RNA-Seq), numerous Th17-associated cytokine genes (including IL-17A, IL-17F, IL-22, IL-19, and IL-27) were upregulated>9-fold in response to purified protein derivative stimulation of peripheral blood mononuclear cells from experimentally M. bovis-infected cattle. Protective vaccines elicited IL-17A, IL-17F, IL-22, and IL-27 responses. Reduced IL-17A responses by vaccine recipients, compared to nonvaccinated animals, at 2.5 weeks after M. bovis challenge correlated with reduced disease burdens. Additionally, IL-17A and interferon gamma (IFN-γ) responses were highly correlated and exhibited similar diagnostic capacities. The present findings support the use of Th17-associated cytokines as biomarkers of infection and protection in the immune responses to bovine tuberculosis.

Original languageEnglish
Pages (from-to)168-180
Number of pages13
JournalClinical and Vaccine Immunology
Issue number2
Publication statusPublished - 05 Feb 2016


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