Abstract
The tetraploid species Avena agadiriana that was first described in 1985 is
distributed on the Atlantic coastal strip south of Casablanca in Morocco. Five
accessions of this species (M55, M59, M60, M71 and M74) were compared by
using FISH and RFLP analysis of 18S-5.8S-26S rDNA. The FISH data indicated
that three pairs of major hybridization sites of the rDNA were located on satellite
chromosomes in accessions M55, M59, M60 and M71. Accession M74, however,
had only two pairs of major sites of hybridization. A pair of the major rDNA
sites in M71 was very small and closely located at the terminal region of Nor-ST
(Nucleolar organizing region of subtelocentrics) chromosomes. RFLP analysis of
the rDNA sequence fragments identified differences among M55, M71 and M74,
whilst M59 and M60 were the same with regard to the four restriction enzyme
fragments utilized. M74 always lost single rDNA fragments in four restriction
enzyme digests. The RFLP data made it possible to distinguish M55 from M59
and M60 in the northern Haut-Atlas Mountains group. A unique 20 kb EcoRI
fragment characterized M71. Thus, a combination of FISH and RFLP analysis of
rDNA was a good tool for inferring intraspecific evolutionary relationship of A.
agadiriana.
Original language | English |
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Pages (from-to) | 9-14 |
Number of pages | 6 |
Journal | Genes and Genetic Systems |
Volume | 76 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2001 |