Introgression of chromosomes of Festuca arundinacea  var. glaucescens into Lolium multiflorum  revealed by genomic in situ  hybridisation (GISH)

John A. Harper; W. Gareth Morgan; Huw M. Thomas; S. Koch; Ian P. King

doi:10.1007/s001220100634

Introgression of chromosomes of Festuca arundinacea var. glaucescens into Lolium multiflorum revealed by genomic in situ hybridisation (GISH)

John A. Harper, W. Gareth Morgan, Huw M. Thomas, S. Koch, Ian P. King

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

An F1 hybrid (n=4x=28) between the tetraploid species Festuca arundinacea var. glaucescens (GGG′G′) and a synthetic tetraploid Lolium multiflorum (LmLmLmLm) was backcrossed to diploid L. multiflorum to produce triploid (2n=3x=21) BC1 hybrids (LmLmG). At metaphase I of meiosis the triploids had a preponderance of ring bivalents and univalents with some linear and frying-pan trivalents. Genomic in situ hybridisation (GISH) differentiated the Festuca chromosomes from Lolium and revealed that the bivalents were exclusively between Lolium homologues, while the univalents were Festuca. Despite the limited amount of homoeologous chiasmata pairing in the triploids, some recombinant chromosomes were recovered in the second backcross when the hybrids were further crossed to diploid L. multiflorum. The progeny from the second backcross was predominantly diploid. Genotypes with recombinant chromosomes and chromosome additions involving an extra Festuca chromosome were identified using GISH. Changes in plant phenotype were related to the presence of Festuca chromatin.

Original language	English
Pages (from-to)	696-701
Number of pages	6
Journal	Theoretical and Applied Genetics
Volume	103
Issue number	5
DOIs	https://doi.org/10.1007/s001220100634
Publication status	Published - Oct 2001

Keywords

Lolium
Festuca
Genomic in situ hybridisation
Introgression
Recombination

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10.1007/s001220100634

http://hdl.handle.net/2160/3559

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@article{e23f1486609f462698baddc64ae88444,

title = "Introgression of chromosomes of Festuca arundinacea var. glaucescens into Lolium multiflorum revealed by genomic in situ hybridisation (GISH)",

abstract = "An F1 hybrid (n=4x=28) between the tetraploid species Festuca arundinacea var. glaucescens (GGG′G′) and a synthetic tetraploid Lolium multiflorum (LmLmLmLm) was backcrossed to diploid L. multiflorum to produce triploid (2n=3x=21) BC1 hybrids (LmLmG). At metaphase I of meiosis the triploids had a preponderance of ring bivalents and univalents with some linear and frying-pan trivalents. Genomic in situ hybridisation (GISH) differentiated the Festuca chromosomes from Lolium and revealed that the bivalents were exclusively between Lolium homologues, while the univalents were Festuca. Despite the limited amount of homoeologous chiasmata pairing in the triploids, some recombinant chromosomes were recovered in the second backcross when the hybrids were further crossed to diploid L. multiflorum. The progeny from the second backcross was predominantly diploid. Genotypes with recombinant chromosomes and chromosome additions involving an extra Festuca chromosome were identified using GISH. Changes in plant phenotype were related to the presence of Festuca chromatin.",

keywords = "Lolium, Festuca, Genomic in situ hybridisation, Introgression, Recombination",

author = "Harper, {John A.} and Morgan, {W. Gareth} and Thomas, {Huw M.} and S. Koch and King, {Ian P.}",

note = "Morgan W. G., King, I. P., Koch, S., Harper, J. A., Thomas, H. M. (2001). Introgression of chromosomes of Festuca arundinacea var. glaucescens into Lolium multiflorum revealed by genomic in situ hybridisation (GISH). Theoretical and Applied Genetics, 103, (5), 696-701.",

year = "2001",

month = oct,

doi = "10.1007/s001220100634",

language = "English",

volume = "103",

pages = "696--701",

journal = "Theoretical and Applied Genetics",

issn = "0040-5752",

publisher = "Springer Nature",

number = "5",

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T1 - Introgression of chromosomes of Festuca arundinacea var. glaucescens into Lolium multiflorum revealed by genomic in situ hybridisation (GISH)

AU - Harper, John A.

AU - Morgan, W. Gareth

AU - Thomas, Huw M.

AU - Koch, S.

AU - King, Ian P.

N1 - Morgan W. G., King, I. P., Koch, S., Harper, J. A., Thomas, H. M. (2001). Introgression of chromosomes of Festuca arundinacea var. glaucescens into Lolium multiflorum revealed by genomic in situ hybridisation (GISH). Theoretical and Applied Genetics, 103, (5), 696-701.

PY - 2001/10

Y1 - 2001/10

N2 - An F1 hybrid (n=4x=28) between the tetraploid species Festuca arundinacea var. glaucescens (GGG′G′) and a synthetic tetraploid Lolium multiflorum (LmLmLmLm) was backcrossed to diploid L. multiflorum to produce triploid (2n=3x=21) BC1 hybrids (LmLmG). At metaphase I of meiosis the triploids had a preponderance of ring bivalents and univalents with some linear and frying-pan trivalents. Genomic in situ hybridisation (GISH) differentiated the Festuca chromosomes from Lolium and revealed that the bivalents were exclusively between Lolium homologues, while the univalents were Festuca. Despite the limited amount of homoeologous chiasmata pairing in the triploids, some recombinant chromosomes were recovered in the second backcross when the hybrids were further crossed to diploid L. multiflorum. The progeny from the second backcross was predominantly diploid. Genotypes with recombinant chromosomes and chromosome additions involving an extra Festuca chromosome were identified using GISH. Changes in plant phenotype were related to the presence of Festuca chromatin.

AB - An F1 hybrid (n=4x=28) between the tetraploid species Festuca arundinacea var. glaucescens (GGG′G′) and a synthetic tetraploid Lolium multiflorum (LmLmLmLm) was backcrossed to diploid L. multiflorum to produce triploid (2n=3x=21) BC1 hybrids (LmLmG). At metaphase I of meiosis the triploids had a preponderance of ring bivalents and univalents with some linear and frying-pan trivalents. Genomic in situ hybridisation (GISH) differentiated the Festuca chromosomes from Lolium and revealed that the bivalents were exclusively between Lolium homologues, while the univalents were Festuca. Despite the limited amount of homoeologous chiasmata pairing in the triploids, some recombinant chromosomes were recovered in the second backcross when the hybrids were further crossed to diploid L. multiflorum. The progeny from the second backcross was predominantly diploid. Genotypes with recombinant chromosomes and chromosome additions involving an extra Festuca chromosome were identified using GISH. Changes in plant phenotype were related to the presence of Festuca chromatin.

KW - Lolium

KW - Festuca

KW - Genomic in situ hybridisation

KW - Introgression

KW - Recombination

U2 - 10.1007/s001220100634

DO - 10.1007/s001220100634

M3 - Article

SN - 0040-5752

VL - 103

SP - 696

EP - 701

JO - Theoretical and Applied Genetics

JF - Theoretical and Applied Genetics

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ER -