Isolation and characterisation of novel starch mutants of oats

Tamara Verhoeven, Brendan Fahy, J. Michael Leggett, Graham Moates, Kay Denyer

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16 Citations (SciVal)

Abstract

Mutagenised grains of the diploid oat, Avena strigosa Schreb., were screened for altered starch granules in the endosperm. Three mutant lines were identified: mutants lam-1 and lam-2 had granules that stained red with iodine solution and sga-1 had endosperm containing soluble material that stained red with iodine solution, as well as blue-staining starch granules. The lam-1 and lam-2 lines lack or have severely reduced amounts of granule-bound starch synthase activity, the granule-bound starch synthase I protein and the amylose component of starch in the endosperm. They therefore represent mutations of the waxy type. The red-staining, soluble material in the sga-1 mutant is very similar in appearance and chain-length profile to the phytoglycogen that accumulates in the endosperms of the sugary-1 mutants of cereals. All sugary-1 mutants lack or have reduced amounts of isoamylase activity. However, native gel assays reveal a normal isoamylase activity in the developing endosperms of sga-1 suggesting that it is not of the sugary-1 type. The nature of the mutation responsible for the sga-1 phenotype has yet to be discovered.
Original languageEnglish
Pages (from-to)69-79
Number of pages11
JournalJournal of Cereal Science
DOIs
Publication statusPublished - Jul 2004
Externally publishedYes

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