Isolation and characterization of a genomic region upstream from the ovine Na+/D-glucose cotransporter (SGLT1) cDNA

I. Stuart Wood, Gordon Allison, Soraya P. Shirazi-Beechey

Research output: Contribution to journalArticlepeer-review

7 Citations (SciVal)

Abstract

d-Glucose and non-metabolisable analogues of D-glucose regulate the expression of intestinal SGLT1 at both transcriptional and post-transcriptional levels. In order to investigate the molecular mechanisms involved in the transcriptional regulation of the ovine intestinal SGLT1 gene, we have isolated an upstream element of about 1 kb in size. This DNA fragment contains a TATA box motif, 48 bp upstream of the transcriptional start site and includes transcription factor binding sites for HNF-1 and AP-2. We have shown that the ovine SGLT1 promoter fragment can drive the transcription of a reporter gene when transfected into the epithelial cell lines STC-1 and LLC-PK1, which endogenously express SGLT1. Deletion analyses of the promoter indicate that −66/+21 bp proximal sequence directs the highest level of reporter gene activity. There are one and possibly two sites of transcriptional suppression.
Original languageEnglish
Pages (from-to)533-537
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume257
Issue number2
DOIs
Publication statusPublished - 13 Apr 1999

Keywords

  • SGLT1
  • promoter
  • transcription
  • ovine intestine
  • Promoter
  • Ovine intestine
  • Transcription

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