Projects per year
Abstract
Miscanthus × giganteus is a source of platform chemicals and bioethanol through fermentation. Cinnamates in leaves and stems were analysed by LC–ESI-MSn. Free phenols were extracted and separated chromatographically. More than 20 hydroxycinnamates were identified by UV and LC–ESI-MSn. Comparative LC–MS studies on the leaf extract showed isomers of O-caffeoylquinic acid (3-CQA, 4-CQA and 5-CQA), O-feruloylquinic acid (3-FQA, 4-FQA and 5-FQA) and para-coumaroylquinic acid (3-pCoQA and 5-pCoQA). Excepting 3-pCoQA, all were also detected in stem. 5-CQA dominated in leaf; a mandelonitrile–caffeoylquinic acid dominated in stem. Three minor leaf components were distinguished by fragmentation patterns in a targetted MS2 experiment as dicaffeoylquinic acid isomers. Others (Mr 516) were tentatively identified as hexosylcaffeoyl-quinates. Three positional isomers of O-caffeoylshikimic acid were minor components. p-Hydroxybenzaldehyde was also a major component in stem. This is the first report of the hydroxycinnamic acid profile of leaves and stems of M. × giganteus.
Original language | English |
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Pages (from-to) | 2376-2384 |
Number of pages | 9 |
Journal | Phytochemistry |
Volume | 72 |
Issue number | 18 |
Early online date | 06 Sept 2011 |
DOIs | |
Publication status | Published - 01 Dec 2011 |
Keywords
- Miscanthus x giganteus leaf and stem tissue
- hydroxycinnamic esters
- UV
- LC-ESI-MSn
- high-value chemicals
- High-value chemicals
- LC-ESI-MS
- Hydroxycinnamic esters
- Miscanthus × giganteus leaf and stem tissue
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Dive into the research topics of 'Isolation, identification and quantitation of hydroxycinnamic acid conjugates, potential platform chemicals, in the leaves and stems of Miscanthus × giganteus using LC–ESI-MSn'. Together they form a unique fingerprint.Projects
- 2 Finished
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Biorefining Centre of Excellence (BEACON)
Fish, S. A. (PI) & Shah, I. P. (CoI)
01 Apr 2010 → 31 Mar 2015
Project: Externally funded research
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Regulation of polyphenolics and genetic manipulation of lignins in grasses
Donnison, I. (PI), Allison, G. (PI) & Bosch, M. (PI)
Biotechnology and Biological Sciences Research Council
01 Apr 2008 → 31 Mar 2012
Project: Externally funded research