Isoniazid treatment of Mycobacterium bovis in cattle as a model for human tuberculosis

G. S. Dean; S. G. Rhodes; M. Coad; A. O. Whelan; P. Wheeler; B. Villareal-Ramos; E. Mead; L. Johnson; D. J. Clifford; R. G. Hewinson; H. M. Vordermeier

doi:10.1016/j.tube.2008.01.004

Isoniazid treatment of Mycobacterium bovis in cattle as a model for human tuberculosis

G. S. Dean^*, S. G. Rhodes, M. Coad, A. O. Whelan, P. Wheeler, B. Villareal-Ramos, E. Mead, L. Johnson, D. J. Clifford, R. G. Hewinson, H. M. Vordermeier

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Cattle infected with Mycobacterium bovis spoligotype 9 were treated with Isoniazid (INH) from three to 14 weeks post infection, rested for four weeks to allow INH depletion and then challenged with M. bovis spoligotype 35. Post mortem examination (PME) 35 weeks after the initial infection showed partial protection against infectious challenge following INH-attenuated infection compared with the spoligotype 35 challenge controls. Antigen-specific IFN-γ responses decreased over time with INH therapy, following a similar pattern to that observed in the treatment of M. tuberculosis infection in humans. Following cessation of therapy, specific IFN-γ responses increased more strongly in those calves that were visibly lesioned at PME. IFN-γ responses were also used to identify two antigens, TB10.4 and Acr2, that induced anamnestic responses in INH-treated, re-challenged calves, suggesting a role for both antigens in protective immunity. Specific IL-10 responses were observed in all calves following treatment with INH suggesting a role for IL-10 in the resolution of infection. Crown

Original language	English
Pages (from-to)	586-594
Number of pages	9
Journal	Tuberculosis
Volume	88
Issue number	6
Early online date	02 May 2008
DOIs	https://doi.org/10.1016/j.tube.2008.01.004
Publication status	Published - 01 Nov 2008

Keywords

Drug-induced
Protection
Tuberculosis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Isoniazid treatment of Mycobacterium bovis in cattle as a model for human tuberculosis",

abstract = "Cattle infected with Mycobacterium bovis spoligotype 9 were treated with Isoniazid (INH) from three to 14 weeks post infection, rested for four weeks to allow INH depletion and then challenged with M. bovis spoligotype 35. Post mortem examination (PME) 35 weeks after the initial infection showed partial protection against infectious challenge following INH-attenuated infection compared with the spoligotype 35 challenge controls. Antigen-specific IFN-γ responses decreased over time with INH therapy, following a similar pattern to that observed in the treatment of M. tuberculosis infection in humans. Following cessation of therapy, specific IFN-γ responses increased more strongly in those calves that were visibly lesioned at PME. IFN-γ responses were also used to identify two antigens, TB10.4 and Acr2, that induced anamnestic responses in INH-treated, re-challenged calves, suggesting a role for both antigens in protective immunity. Specific IL-10 responses were observed in all calves following treatment with INH suggesting a role for IL-10 in the resolution of infection. Crown",

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AU - Dean, G. S.

AU - Rhodes, S. G.

AU - Coad, M.

AU - Whelan, A. O.

AU - Wheeler, P.

AU - Villareal-Ramos, B.

AU - Mead, E.

AU - Johnson, L.

AU - Clifford, D. J.

AU - Hewinson, R. G.

AU - Vordermeier, H. M.

PY - 2008/11/1

Y1 - 2008/11/1

N2 - Cattle infected with Mycobacterium bovis spoligotype 9 were treated with Isoniazid (INH) from three to 14 weeks post infection, rested for four weeks to allow INH depletion and then challenged with M. bovis spoligotype 35. Post mortem examination (PME) 35 weeks after the initial infection showed partial protection against infectious challenge following INH-attenuated infection compared with the spoligotype 35 challenge controls. Antigen-specific IFN-γ responses decreased over time with INH therapy, following a similar pattern to that observed in the treatment of M. tuberculosis infection in humans. Following cessation of therapy, specific IFN-γ responses increased more strongly in those calves that were visibly lesioned at PME. IFN-γ responses were also used to identify two antigens, TB10.4 and Acr2, that induced anamnestic responses in INH-treated, re-challenged calves, suggesting a role for both antigens in protective immunity. Specific IL-10 responses were observed in all calves following treatment with INH suggesting a role for IL-10 in the resolution of infection. Crown

AB - Cattle infected with Mycobacterium bovis spoligotype 9 were treated with Isoniazid (INH) from three to 14 weeks post infection, rested for four weeks to allow INH depletion and then challenged with M. bovis spoligotype 35. Post mortem examination (PME) 35 weeks after the initial infection showed partial protection against infectious challenge following INH-attenuated infection compared with the spoligotype 35 challenge controls. Antigen-specific IFN-γ responses decreased over time with INH therapy, following a similar pattern to that observed in the treatment of M. tuberculosis infection in humans. Following cessation of therapy, specific IFN-γ responses increased more strongly in those calves that were visibly lesioned at PME. IFN-γ responses were also used to identify two antigens, TB10.4 and Acr2, that induced anamnestic responses in INH-treated, re-challenged calves, suggesting a role for both antigens in protective immunity. Specific IL-10 responses were observed in all calves following treatment with INH suggesting a role for IL-10 in the resolution of infection. Crown

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KW - Protection

KW - Tuberculosis

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ER -

Isoniazid treatment of Mycobacterium bovis in cattle as a model for human tuberculosis

Abstract

Keywords

UN SDGs

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