Isothermal Amplification of Genetically Modified DNA Sequences Directly from Plant Tissues Lowers the Barriers to High-Throughput and Field-Based Genotyping

David Lee; Maurizio  La Mura; Theo Allnutt; Wayne Powell; Andy Greenland

doi:10.1021/jf902455j

Isothermal Amplification of Genetically Modified DNA Sequences Directly from Plant Tissues Lowers the Barriers to High-Throughput and Field-Based Genotyping

David Lee, Maurizio La Mura, Theo Allnutt, Wayne Powell, Andy Greenland

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

DNA extractions are a major cost for high-throughput genotyping. The loop-mediated isothermal amplification (LAMP) assay has been used for the detection of two genetically modified (GM) related sequences. The amplification of target DNA sequences from leaf and maize seed tissues prepared with minimum preparative treatment (disruption in water) demonstrates the ability of LAMP to work in conditions normally inhibitive to PCRs. The wide dynamic range of detection in these samples suggests that LAMP is highly sensitive even when the target is presented in such a crude form. LAMP offers a means of reducing genotyping costs as well as simplifying testing procedures.

Original language	English
Pages (from-to)	9400-9402
Number of pages	3
Journal	Journal of Agricultural and Food Chemistry
Volume	57
Issue number	20
DOIs	https://doi.org/10.1021/jf902455j
Publication status	Published - 22 Sept 2009

Keywords

Loop-mediated isothermal amplification (LAMP)
GM detection
RF3 oilseed rape
leaf tissue
MON810 maize seed

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10.1021/jf902455j

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title = "Isothermal Amplification of Genetically Modified DNA Sequences Directly from Plant Tissues Lowers the Barriers to High-Throughput and Field-Based Genotyping",

abstract = "DNA extractions are a major cost for high-throughput genotyping. The loop-mediated isothermal amplification (LAMP) assay has been used for the detection of two genetically modified (GM) related sequences. The amplification of target DNA sequences from leaf and maize seed tissues prepared with minimum preparative treatment (disruption in water) demonstrates the ability of LAMP to work in conditions normally inhibitive to PCRs. The wide dynamic range of detection in these samples suggests that LAMP is highly sensitive even when the target is presented in such a crude form. LAMP offers a means of reducing genotyping costs as well as simplifying testing procedures.",

keywords = "Loop-mediated isothermal amplification (LAMP), GM detection, RF3 oilseed rape, leaf tissue, MON810 maize seed",

author = "David Lee and {La Mura}, Maurizio and Theo Allnutt and Wayne Powell and Andy Greenland",

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Isothermal Amplification of Genetically Modified DNA Sequences Directly from Plant Tissues Lowers the Barriers to High-Throughput and Field-Based Genotyping. / Lee, David; La Mura, Maurizio ; Allnutt, Theo et al.
In: Journal of Agricultural and Food Chemistry, Vol. 57, No. 20, 22.09.2009, p. 9400-9402.

Research output: Contribution to journal › Article › peer-review

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T1 - Isothermal Amplification of Genetically Modified DNA Sequences Directly from Plant Tissues Lowers the Barriers to High-Throughput and Field-Based Genotyping

AU - Lee, David

AU - La Mura, Maurizio

AU - Allnutt, Theo

AU - Powell, Wayne

AU - Greenland, Andy

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KW - GM detection

KW - RF3 oilseed rape

KW - leaf tissue

KW - MON810 maize seed

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ER -

Isothermal Amplification of Genetically Modified DNA Sequences Directly from Plant Tissues Lowers the Barriers to High-Throughput and Field-Based Genotyping

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Keywords

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