Licensing MLH1 sites for crossover during meiosis

Azahara C. Martin, Peter Shaw, Dylan Phillips, Steve Reader, Graham Moore

Research output: Contribution to journalArticlepeer-review

59 Citations (SciVal)
138 Downloads (Pure)

Abstract

During meiosis, homologous chromosomes synapse and recombine at sites marked by the binding of the mismatch repair protein MLH1. In hexaploid wheat, the Ph1 locus has a major effect on whether crossover occurs between homologues or between related homoeologues. Here we report that—in wheat–rye hybrids where homologues are absent—Ph1 affects neither the level of synapsis nor the number of MLH1. Thus in the case of wheat–wild relative hybrids, Ph1 must affect whether MLH1 sites are able to progress to crossover. The observed level of synapsis implies that Ph1 functions to promote homologue pairing rather than suppress homoeologue pairing in wheat. Therefore, Ph1 stabilises polyploidy in wheat by both promoting homologue pairing and preventing MLH1 sites from becoming crossovers on paired homoeologues during meiosis.
Original languageEnglish
Article number4580
Number of pages5
JournalNature Communications
Volume5
DOIs
Publication statusPublished - 06 Aug 2014

Keywords

  • Acrylic Resins/chemistry
  • Adenosine Triphosphatases/genetics
  • Arabidopsis Proteins/genetics
  • Arabidopsis/genetics
  • Cell Cycle
  • Chromosomes, Plant
  • Crossing Over, Genetic
  • DNA Repair
  • Genes, Plant
  • Genetic Techniques
  • Genome, Plant
  • Image Processing, Computer-Assisted
  • Meiosis/genetics
  • Microscopy, Confocal
  • MutL Protein Homolog 1
  • Plant Proteins/genetics
  • Polyploidy
  • Recombination, Genetic
  • Secale/genetics
  • Seeds/metabolism
  • Triticum/genetics

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