TY - JOUR
T1 - Metabolic molecular markers of the tidal clock in the marine crustacean Eurydice pulchra
AU - O'Neill, John Stuart
AU - Lee, Kate D.
AU - Zhang, Lin
AU - Feeney, Kevin
AU - Webster, Simon George
AU - Blades, James
AU - Kyriacou, Charalambos Panayiotis
AU - Hastings, Michael
AU - Wilcockson, David
N1 - O'Neill, J. S., Lee, K. D., Zhang, L., Feeney, K., Webster, S. G., Blades, J., Kyriacou, C. P., Hastings, M. & Wilcockson, D. (2015). Metabolic molecular markers of the tidal clock in the marine crustacean Eurydice pulchra. Current Biology, 25 (8), R326-R327
PY - 2015/4/20
Y1 - 2015/4/20
N2 - In contrast to the well mapped molecular orchestration of circadian timekeeping in terrestrial organisms, the mechanisms that direct tidal and lunar rhythms in marine species are entirely unknown. Using a combination of biochemical and molecular approaches we have identified a series of metabolic markers of the tidal clock of the intertidal isopod Eurydice pulchra. Specifically, we show that the overoxidation of peroxiredoxin (PRX), a conserved marker of circadian timekeeping in terrestrial eukaryotes [1], follows a circatidal (approximately 12.4 hours) pattern in E. pulchra, in register with the tidal pattern of swimming. In parallel, we show that mitochondrially encoded genes are expressed with a circatidal rhythm. Together, these findings demonstrate that PRX overoxidation rhythms are not intrinsically circadian; rather they appear to resonate with the dominant metabolic cycle of an organism, regardless of its frequency. Moreover, they provide the first molecular leads for dissecting the tidal clockwork.
AB - In contrast to the well mapped molecular orchestration of circadian timekeeping in terrestrial organisms, the mechanisms that direct tidal and lunar rhythms in marine species are entirely unknown. Using a combination of biochemical and molecular approaches we have identified a series of metabolic markers of the tidal clock of the intertidal isopod Eurydice pulchra. Specifically, we show that the overoxidation of peroxiredoxin (PRX), a conserved marker of circadian timekeeping in terrestrial eukaryotes [1], follows a circatidal (approximately 12.4 hours) pattern in E. pulchra, in register with the tidal pattern of swimming. In parallel, we show that mitochondrially encoded genes are expressed with a circatidal rhythm. Together, these findings demonstrate that PRX overoxidation rhythms are not intrinsically circadian; rather they appear to resonate with the dominant metabolic cycle of an organism, regardless of its frequency. Moreover, they provide the first molecular leads for dissecting the tidal clockwork.
UR - http://hdl.handle.net/2160/28239
UR - https://ars.els-cdn.com/content/image/1-s2.0-S0960982215002213-mmc1.pdf
U2 - 10.1016/j.cub.2015.02.052
DO - 10.1016/j.cub.2015.02.052
M3 - Article
C2 - 25898100
SN - 1879-0445
VL - 25
SP - R326-R327
JO - Current Biology
JF - Current Biology
IS - 8
ER -