Methane oxidation and its coupled electron-sink reactions in ruminal fluid

H. Kajikawa, C. Valdes, K. Hillman, R. J. Wallace, C. J. Newbold

Research output: Contribution to journalArticlepeer-review

25 Citations (SciVal)

Abstract

Aims: This study was conducted to investigate the occurrence of methane oxidation in the rumen, and to identify the electron-sink reaction coupled to the oxidation if it occurred.

Methods and Results: Mixed ruminal microbes taken from sheep were incubated with (CH4)-C-13 . Oxidation of methane, estimated from the flux of C-13 to CO2 and microbial cells, occurred, but represented only 0.2-0.5% of the methane produced. Methane oxidation was suppressed by the presence of oxygen, and was also inhibited by 2-bromoethane-sulphonate, and molybdate, but not by tungstate.

Conclusion, Significance and Impact of the Study: Methane could be oxidized anaerobically in the rumen by reverse methanogenesis in consort with sulphate reduction.

Original languageEnglish
Pages (from-to)354-357
Number of pages4
JournalLetters in Applied Microbiology
Volume36
Issue number6
Early online date12 May 2003
DOIs
Publication statusPublished - Jun 2003

Keywords

  • OVINE RUMEN
  • SULFATE REDUCTION
  • oxygen
  • SAMPLES
  • rumen
  • nitrate reduction
  • METHANOGENIC BACTERIA
  • methane oxidation
  • MIXED RUMEN MICROORGANISMS
  • SEDIMENTS
  • sulphate reduction

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