Methane oxidation and its coupled electron-sink reactions in ruminal fluid

H. Kajikawa; C. Valdes; K. Hillman; R. J. Wallace; C. J. Newbold

doi:10.1046/j.1472-765X.2003.01317.x

Methane oxidation and its coupled electron-sink reactions in ruminal fluid

H. Kajikawa, C. Valdes, K. Hillman, R. J. Wallace, C. J. Newbold

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Aims: This study was conducted to investigate the occurrence of methane oxidation in the rumen, and to identify the electron-sink reaction coupled to the oxidation if it occurred.

Methods and Results: Mixed ruminal microbes taken from sheep were incubated with (CH4)-C-13 . Oxidation of methane, estimated from the flux of C-13 to CO2 and microbial cells, occurred, but represented only 0.2-0.5% of the methane produced. Methane oxidation was suppressed by the presence of oxygen, and was also inhibited by 2-bromoethane-sulphonate, and molybdate, but not by tungstate.

Conclusion, Significance and Impact of the Study: Methane could be oxidized anaerobically in the rumen by reverse methanogenesis in consort with sulphate reduction.

Original language	English
Pages (from-to)	354-357
Number of pages	4
Journal	Letters in Applied Microbiology
Volume	36
Issue number	6
Early online date	12 May 2003
DOIs	https://doi.org/10.1046/j.1472-765X.2003.01317.x
Publication status	Published - Jun 2003

Keywords

OVINE RUMEN
SULFATE REDUCTION
oxygen
SAMPLES
rumen
nitrate reduction
METHANOGENIC BACTERIA
methane oxidation
MIXED RUMEN MICROORGANISMS
SEDIMENTS
sulphate reduction

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title = "Methane oxidation and its coupled electron-sink reactions in ruminal fluid",

abstract = "Aims: This study was conducted to investigate the occurrence of methane oxidation in the rumen, and to identify the electron-sink reaction coupled to the oxidation if it occurred.Methods and Results: Mixed ruminal microbes taken from sheep were incubated with (CH4)-C-13 . Oxidation of methane, estimated from the flux of C-13 to CO2 and microbial cells, occurred, but represented only 0.2-0.5% of the methane produced. Methane oxidation was suppressed by the presence of oxygen, and was also inhibited by 2-bromoethane-sulphonate, and molybdate, but not by tungstate.Conclusion, Significance and Impact of the Study: Methane could be oxidized anaerobically in the rumen by reverse methanogenesis in consort with sulphate reduction.",

keywords = "OVINE RUMEN, SULFATE REDUCTION, oxygen, SAMPLES, rumen, nitrate reduction, METHANOGENIC BACTERIA, methane oxidation, MIXED RUMEN MICROORGANISMS, SEDIMENTS, sulphate reduction",

author = "H. Kajikawa and C. Valdes and K. Hillman and Wallace, {R. J.} and Newbold, {C. J.}",

note = "Kajikawa, H., Valdes, C., Hillman, K., Wallace, R. J., Newbold, C. J. (2003). Methane oxidation and its coupled electron-sink reactions in ruminal fluid. Letters in Applied Microbiology, 36 (6), 354-357.",

year = "2003",

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doi = "10.1046/j.1472-765X.2003.01317.x",

language = "English",

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pages = "354--357",

journal = "Letters in Applied Microbiology",

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TY - JOUR

T1 - Methane oxidation and its coupled electron-sink reactions in ruminal fluid

AU - Kajikawa, H.

AU - Valdes, C.

AU - Hillman, K.

AU - Wallace, R. J.

AU - Newbold, C. J.

N1 - Kajikawa, H., Valdes, C., Hillman, K., Wallace, R. J., Newbold, C. J. (2003). Methane oxidation and its coupled electron-sink reactions in ruminal fluid. Letters in Applied Microbiology, 36 (6), 354-357.

PY - 2003/6

Y1 - 2003/6

N2 - Aims: This study was conducted to investigate the occurrence of methane oxidation in the rumen, and to identify the electron-sink reaction coupled to the oxidation if it occurred.Methods and Results: Mixed ruminal microbes taken from sheep were incubated with (CH4)-C-13 . Oxidation of methane, estimated from the flux of C-13 to CO2 and microbial cells, occurred, but represented only 0.2-0.5% of the methane produced. Methane oxidation was suppressed by the presence of oxygen, and was also inhibited by 2-bromoethane-sulphonate, and molybdate, but not by tungstate.Conclusion, Significance and Impact of the Study: Methane could be oxidized anaerobically in the rumen by reverse methanogenesis in consort with sulphate reduction.

AB - Aims: This study was conducted to investigate the occurrence of methane oxidation in the rumen, and to identify the electron-sink reaction coupled to the oxidation if it occurred.Methods and Results: Mixed ruminal microbes taken from sheep were incubated with (CH4)-C-13 . Oxidation of methane, estimated from the flux of C-13 to CO2 and microbial cells, occurred, but represented only 0.2-0.5% of the methane produced. Methane oxidation was suppressed by the presence of oxygen, and was also inhibited by 2-bromoethane-sulphonate, and molybdate, but not by tungstate.Conclusion, Significance and Impact of the Study: Methane could be oxidized anaerobically in the rumen by reverse methanogenesis in consort with sulphate reduction.

KW - OVINE RUMEN

KW - SULFATE REDUCTION

KW - oxygen

KW - SAMPLES

KW - rumen

KW - nitrate reduction

KW - METHANOGENIC BACTERIA

KW - methane oxidation

KW - MIXED RUMEN MICROORGANISMS

KW - SEDIMENTS

KW - sulphate reduction

UR - http://hdl.handle.net/2160/9403

U2 - 10.1046/j.1472-765X.2003.01317.x

DO - 10.1046/j.1472-765X.2003.01317.x

M3 - Article

SN - 0266-8254

VL - 36

SP - 354

EP - 357

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

IS - 6

ER -

Methane oxidation and its coupled electron-sink reactions in ruminal fluid

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