Methods for Delivery of dsRNAi Against Canonical Clock Genes and Immunocytodetection of Clock Proteins in Crustacea

David C. Wilcockson, Lin Zhang, Charalambos P. Kyriacou*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (SciVal)

Abstract

The use of nonclassical model organisms for biological rhythm research has become popular in the last two decades. Here we describe techniques for delivery of dsRNAi molecules to knock down clock gene transcripts in a small intertidal crustacean, Eurydice pulchra, as well as our method for immunodetection of clock proteins in the brain. These methods can be generalized for gene knockdown in any small crustacean or arthropod in which mutagenesis by other methods is neither practical nor possible.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages385-394
Number of pages10
ISBN (Electronic)978-1-0716-2249-0
ISBN (Print)978-1-0716-2248-3
DOIs
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2482
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Clock genes
  • Crustacea
  • dsRNAi
  • Immunocytochemistry
  • Microinjection

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