Microarray analysis of Mycobacterium microti reveals deletion of genes encoding PE-PPE proteins and ESAT-6 family antigens

M. Carmen Garcia-Pelayo; Karina C. Caimi; Jacqueline K. Inwald; Jason Hinds; Fabiana Bigi; Maria I. Romano; Dick Van Soolingen; R. Glyn Hewinson; Angel Cataldi; Stephen V. Gordon

doi:10.1016/j.tube.2003.12.002

Microarray analysis of Mycobacterium microti reveals deletion of genes encoding PE-PPE proteins and ESAT-6 family antigens

M. Carmen Garcia-Pelayo, Karina C. Caimi, Jacqueline K. Inwald, Jason Hinds, Fabiana Bigi, Maria I. Romano, Dick Van Soolingen, R. Glyn Hewinson, Angel Cataldi, Stephen V. Gordon^*

^*Corresponding author for this work

Bovine TB, diseases and control

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Mycobacterium microti is the agent of tuberculosis in wild voles and has been used as a live vaccine against tuberculosis in man and cattle. To explore the M. microti genome in greater detail, we used a M. tuberculosis H37Rv genomic DNA microarray to detect gene deletions among M. microti isolates. A number of deletions were identified that correlated with those described previously (Infect. Immun. 70 (2002) 5568) but a novel M. microti deletion was also found (MiD4) which removes 5 genes that code for ESAT-6 family antigens and PE-PPE proteins. Southern blot experiments showed that this region was also deleted from M. pinnipedii, a mycobacterium isolated from seals that is closely related to M. microti. Genes encoding ESAT-6 antigens and PE-PPE proteins appear to be frequently deleted from M. microti, and the implications of this are discussed.

Original language	English
Pages (from-to)	159-166
Number of pages	8
Journal	Tuberculosis
Volume	84
Issue number	3-4
Early online date	16 Apr 2004
DOIs	https://doi.org/10.1016/j.tube.2003.12.002
Publication status	Published - 14 Jul 2004

Keywords

ESAT-6
Mycobacterium microti
PPE
Tuberculosis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.tube.2003.12.002

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title = "Microarray analysis of Mycobacterium microti reveals deletion of genes encoding PE-PPE proteins and ESAT-6 family antigens",

abstract = "Mycobacterium microti is the agent of tuberculosis in wild voles and has been used as a live vaccine against tuberculosis in man and cattle. To explore the M. microti genome in greater detail, we used a M. tuberculosis H37Rv genomic DNA microarray to detect gene deletions among M. microti isolates. A number of deletions were identified that correlated with those described previously (Infect. Immun. 70 (2002) 5568) but a novel M. microti deletion was also found (MiD4) which removes 5 genes that code for ESAT-6 family antigens and PE-PPE proteins. Southern blot experiments showed that this region was also deleted from M. pinnipedii, a mycobacterium isolated from seals that is closely related to M. microti. Genes encoding ESAT-6 antigens and PE-PPE proteins appear to be frequently deleted from M. microti, and the implications of this are discussed.",

keywords = "ESAT-6, Mycobacterium microti, PPE, Tuberculosis",

author = "Garcia-Pelayo, {M. Carmen} and Caimi, {Karina C.} and Inwald, {Jacqueline K.} and Jason Hinds and Fabiana Bigi and Romano, {Maria I.} and {Van Soolingen}, Dick and Hewinson, {R. Glyn} and Angel Cataldi and Gordon, {Stephen V.}",

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AU - Garcia-Pelayo, M. Carmen

AU - Caimi, Karina C.

AU - Inwald, Jacqueline K.

AU - Hinds, Jason

AU - Bigi, Fabiana

AU - Romano, Maria I.

AU - Van Soolingen, Dick

AU - Hewinson, R. Glyn

AU - Cataldi, Angel

AU - Gordon, Stephen V.

PY - 2004/7/14

Y1 - 2004/7/14

N2 - Mycobacterium microti is the agent of tuberculosis in wild voles and has been used as a live vaccine against tuberculosis in man and cattle. To explore the M. microti genome in greater detail, we used a M. tuberculosis H37Rv genomic DNA microarray to detect gene deletions among M. microti isolates. A number of deletions were identified that correlated with those described previously (Infect. Immun. 70 (2002) 5568) but a novel M. microti deletion was also found (MiD4) which removes 5 genes that code for ESAT-6 family antigens and PE-PPE proteins. Southern blot experiments showed that this region was also deleted from M. pinnipedii, a mycobacterium isolated from seals that is closely related to M. microti. Genes encoding ESAT-6 antigens and PE-PPE proteins appear to be frequently deleted from M. microti, and the implications of this are discussed.

AB - Mycobacterium microti is the agent of tuberculosis in wild voles and has been used as a live vaccine against tuberculosis in man and cattle. To explore the M. microti genome in greater detail, we used a M. tuberculosis H37Rv genomic DNA microarray to detect gene deletions among M. microti isolates. A number of deletions were identified that correlated with those described previously (Infect. Immun. 70 (2002) 5568) but a novel M. microti deletion was also found (MiD4) which removes 5 genes that code for ESAT-6 family antigens and PE-PPE proteins. Southern blot experiments showed that this region was also deleted from M. pinnipedii, a mycobacterium isolated from seals that is closely related to M. microti. Genes encoding ESAT-6 antigens and PE-PPE proteins appear to be frequently deleted from M. microti, and the implications of this are discussed.

KW - ESAT-6

KW - Mycobacterium microti

KW - PPE

KW - Tuberculosis

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DO - 10.1016/j.tube.2003.12.002

M3 - Article

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AN - SCOPUS:3042706681

SN - 1472-9792

VL - 84

SP - 159

EP - 166

JO - Tuberculosis

JF - Tuberculosis

IS - 3-4

ER -

Microarray analysis of Mycobacterium microti reveals deletion of genes encoding PE-PPE proteins and ESAT-6 family antigens

Abstract

Keywords

UN SDGs

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