Microarray analysis of Mycobacterium microti reveals deletion of genes encoding PE-PPE proteins and ESAT-6 family antigens

M. Carmen Garcia-Pelayo, Karina C. Caimi, Jacqueline K. Inwald, Jason Hinds, Fabiana Bigi, Maria I. Romano, Dick Van Soolingen, R. Glyn Hewinson, Angel Cataldi, Stephen V. Gordon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Citations (SciVal)

Abstract

Mycobacterium microti is the agent of tuberculosis in wild voles and has been used as a live vaccine against tuberculosis in man and cattle. To explore the M. microti genome in greater detail, we used a M. tuberculosis H37Rv genomic DNA microarray to detect gene deletions among M. microti isolates. A number of deletions were identified that correlated with those described previously (Infect. Immun. 70 (2002) 5568) but a novel M. microti deletion was also found (MiD4) which removes 5 genes that code for ESAT-6 family antigens and PE-PPE proteins. Southern blot experiments showed that this region was also deleted from M. pinnipedii, a mycobacterium isolated from seals that is closely related to M. microti. Genes encoding ESAT-6 antigens and PE-PPE proteins appear to be frequently deleted from M. microti, and the implications of this are discussed.

Original languageEnglish
Pages (from-to)159-166
Number of pages8
JournalTuberculosis
Volume84
Issue number3-4
Early online date16 Apr 2004
DOIs
Publication statusPublished - 14 Jul 2004

Keywords

  • ESAT-6
  • Mycobacterium microti
  • PPE
  • Tuberculosis

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