Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

MISEV Consortium

doi:10.1002/jev2.12404

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

MISEV Consortium

Research output: Contribution to journal › Article › peer-review

1035 Citations (Scopus)

Abstract

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

Original language	English
Article number	e12404
Journal	Journal of Extracellular Vesicles
Volume	13
Issue number	2
DOIs	https://doi.org/10.1002/jev2.12404
Publication status	Published - 07 Feb 2024

Keywords

ectosomes
exosomes
extracellular particles
extracellular vesicles
guidelines
microparticles
microvesicles
minimal information requirements
MISEV
reproducibility
rigor
standardisation
Exosomes/metabolism
Biomarkers/metabolism
Extracellular Vesicles/metabolism
Phenotype
Biological Transport

Access to Document

10.1002/jev2.12404Licence: CC BY

Permanent link

Persistent link

Cite this

@article{beac4d058679452faf979b3997036449,

title = "Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches",

abstract = "Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its {\textquoteleft}Minimal Information for Studies of Extracellular Vesicles{\textquoteright}, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.",

keywords = "ectosomes, exosomes, extracellular particles, extracellular vesicles, guidelines, microparticles, microvesicles, minimal information requirements, MISEV, reproducibility, rigor, standardisation, Exosomes/metabolism, Biomarkers/metabolism, Extracellular Vesicles/metabolism, Phenotype, Biological Transport",

author = "{MISEV Consortium} and Welsh, {Joshua A.} and Goberdhan, {Deborah C.I.} and Lorraine O{\textquoteright}Driscoll and Buzas, {Edit I.} and Cherie Blenkiron and Benedetta Bussolati and Houjian Cai and {Di Vizio}, Dolores and Driedonks, {Tom A.P.} and Uta Erdbr{\"u}gger and Falcon-Perez, {Juan M.} and Fu, {Qing Ling} and Hill, {Andrew F.} and Metka Lenassi and Lim, {Sai Kiang} and M˜y, {G. Mahoney} and Sujata Mohanty and Andreas M{\"o}ller and Rienk Nieuwland and Takahiro Ochiya and Susmita Sahoo and Torrecilhas, {Ana C.} and Lei Zheng and Andries Zijlstra and Sarah Abuelreich and Reem Bagabas and Paolo Bergese and Bridges, {Esther M.} and Marco Brucale and Dylan Burger and Carney, {Randy P.} and Emanuele Cocucci and Rossella Crescitelli and Edveena Hanser and Harris, {Adrian L.} and Haughey, {Norman J.} and An Hendrix and Ivanov, {Alexander R.} and Tijana Jovanovic-Talisman and Kruh-Garcia, {Nicole A.} and Faustino, {Vroniqa Ku{\textquoteright}ulei Lyn} and Diego Kyburz and Cecilia L{\"a}sser and Lennon, {Kathleen M.} and Jan L{\"o}tvall and Maddox, {Adam L.} and Martens-Uzunova, {Elena S.} and Mizenko, {Rachel R.} and Newman, {Lauren A.} and Andrea Ridolfi and Eva Rohde and Tatu Rojalin and Andrew Rowland and Andras Saftics and Sandau, {Ursula S.} and Saugstad, {Julie A.} and Faezeh Shekari and Simon Swift and Dmitry Ter-Ovanesyan and Tosar, {Juan P.} and Zivile Useckaite and Francesco Valle and Zoltan Varga and {van der Pol}, Edwin and {van Herwijnen}, {Martijn J.C.} and Wauben, {Marca H.M.} and Wehman, {Ann M.} and Sarah Williams and Andrea Zendrini and Zimmerman, {Alan J.} and Clotilde Thery and Witwer, {Kenneth W.} and Samar Ahmad and Ahmed, {Dina A.K.} and Ahmed, {Sarah H.} and Elena Aikawa and Naveed Akbar and Kazunari Akiyoshi and Al-Adra, {David P.} and Al-Masawa, {Maimonah E.} and Manuel Albanese and Ainhoa Alberro and Alcaraz, {Mar{\'i}a Jos{\'e}} and Jen Alexander-Brett and Alexander, {Kimberley L.} and Nilufar Ali and Alibhai, {Faisal J.} and Susann Allelein and Allenby, {Mark C.} and Fausto Almeida and {de Almeida}, {Luis Pereira} and Almousa, {Sameh W.} and Nihal Altan-Bonnet and Altei, {Wanessa F.} and Gloria Alvarez-Llamas and Alvarez, {Cora L.} and An, {Hyo Jung} and Krishnan Anand and Andaloussi, {Samir E.L.} and Anderson, {Johnathon D.} and Ramaroson Andriantsitohaina and Ansari, {Khairul I.} and Achille Anselmo and Anna Antoniou and Farrukh Aqil and Tanina Arab and Fabienne Archer and Syrine Arif and Armstrong, {David A.} and Arntz, {Onno J.} and Pierre Ars{\`e}ne and Luis Arteaga-Blanco and Nandini Asokan and Trude Aspelin and Atkin-Smith, {Georgia K.} and Dimitri Aubert and Ayyar, {Kanchana K.} and Maryam Azlan and Ioannis Azoidis and Ana{\"i}s B{\'e}cot and Bach, {Jean Marie} and Daniel Bachurski and Seoyoon Bae and Bagge, {Roger Olofsson} and Monika Baj-Krzyworzeka and Leonora Balaj and Carolina Balbi and {van Balkom}, {Bas W.M.} and Ballal, {Abhijna R.} and Afsareen Bano and S{\'e}bastien Banzet and Yonis Bare and Lucio Barile and Bahnisikha Barman and Isabel Barranco and Valeria Barreca and Genevi{\`e}ve Bart and Barteneva, {Natasha S.} and Manuela Basso and Mona Batish and Bauer, {Natalie R.} and Baxter, {Amy A.} and Bazi{\'e}, {Wilfried W.} and Erica Bazzan and Beaumont, {Joel E.J.} and Mary Bebawy and Bebelman, {Maarten P.} and Apolonija Bedina-Zavec and Beetler, {Danielle J.} and Tam{\'a}s Beke-Somfai and Cl{\'e}mence Belleann{\'e}e and Benedikter, {Birke J.} and Benediktsd{\'o}ttir, {Berglind E.} and Berardi, {Anna C.} and Mathilde Bergamelli and Irene Bertolini and Asima Bhattacharyya and Bhattacharyya, {Suvendra N.} and Biller, {Steven J.} and Clotilde Billottet and Bissler, {John J.} and Olivier Blanc-Brude and Blijdorp, {Charles J.} and Sylwia Bobis-Wozowicz and Victor Bodart-Santos and Bodn{\'a}r, {Bernadett R.} and Eric Boilard and Wilfrid Boireau and Vladimir Bokun and Bollard, {Stephanie M.} and Sveva Bollini and Antonella Bongiovanni and Laura Bongiovanni and Amandine Bonifay and Boppart, {Marni D.} and Francesce Borr{\`a}s and Steffi Bosch and Daniela Boselli and Massimo Bottini and Jeff Bouffard and Boulanger, {Chantal M.} and Boutros, {Paul C.} and Oscar Boyadjian and Boysen, {Anders T.} and Bozkurt, {Batuhan T.} and Bramich, {Kyle P.} and Fabian Braun and Bravo-Miana, {Roc{\'i}o Del Carmen} and Breakefield, {Xandra O.} and Santra Brenna and Kieran Brennan and Meadhbh Brennan and Koen Breyne and Brigstock, {David R.} and Brisson, {Alain R.} and Chaya Brodie and Katelyna Bruno and Cecilia Bucci and Shilpa Buch and Amyh Buck and M{\'a}ty{\'a}s Bukva and Bulte, {Jeff W.M.} and Sandra Buratta and Olivier Burgy and Burnier, {Julia V.} and Kaiping Burrows and Sara Busatto and Krisztina Buzas and Byrd, {J. Brian} and Albano C{\'a}ceres-Verschae and Caires, {Hugo R.} and Carmen Campos-Silva and Giovanni Camussi and {de Candia}, Paola and Carmen Carceller and Carmen Fernandez-Becerra and Carrasco, {Alexis G.Murillo} and Carter, {David R.F.} and Sara Cavallaro and Serena Cavallero and Sophie Cavallero and Crist{\'o}bal Cerda-Troncoso and Richard Chahwan and Renata Chalupsk{\'a} and Chamley, {Lawrence W.} and Chandra, {Partha K.} and Chang, {Wen Wei} and Al Charest and Chihchen Chen and Hao Chen and Qiang Chen and Shuai Chen and Siyu Chen and Yunxi Chen and Lesley Cheng and Chernyshev, {Vasiliy S.} and Chetty, {Venkatesh Kumar} and Chitti, {Sai V.} and Cho, {Ssang Goo} and Cho, {Yoon Kyoung} and Choi, {Byeong Hyeon} and Somchai Chutipongtanate and Cicardi, {Maria Elena} and Anna Cifuentes-Rius and Alessandra Ciullo and Aled Clayton and Cleary, {Jacob A.} and Federico Cocozza and Coffey, {Robert J.} and Federica Collino and Federico Colombo and Pascal Colosetti and Alvaro Compa{\~n}-Bertomeu and Julie Constanzo and Denis Corbeil and Anabela Cordeiro-Da-Silva and J{\'u}lia Costa and Yvonne Couch and Yvan Courageux and Kelly Coutant and Beth Coyle and Marina Cretich and Andr{\'e} Cronemberger-Andrade and Crossland, {Rachel E.} and Cucher, {Marcela A.} and Malgorzata Czystowska-Kuzmicz and Pasquale D{\textquoteright}Acunzo and Igea D{\textquoteright}Agnano and D{\textquoteright}agostino, {Vito G.} and Daniele D{\textquoteright}Arrigo and Crislyn D{\textquoteright}Souza-Schorey and Dagur, {Raghubendra S.} and Danielson, {Kirsty M.} and Saumya Das and Thibaud Dauphin and Davidson, {Sean M.} and Davies, {Owen G.} and Davies, {Rebecca L.} and Davis, {Chelsea N.} and Gagan Deep and Jonathan Degosserie and {Van Delen}, Mats and Vatsal Deliwala and Dellar, {Elizabeth R.} and {Van Deun}, Jan and Apurba Dev and Sarah Deville and Andrew Devitt and Bert Dhondt and Dieterich, {Lothar C.} and Dittmer, {Dirk P.} and Brian Dobosh and Gabriella Dobra and Navneet Dogra and Eisuke Dohi and Vincenza Dolo and Domashevich, {Timothy V.} and Massimo Dominici and Liang Dong and Etienne Dor{\'e} and Dragovic, {Rebecca A.} and Lila Drittanti and Marvin Droste and Wei Duan and Esmahan Durmaz and Suman Dutta and Takanori Eguchi and Eichenberger, {Ramon M.} and Erez Eitan and Karin Ekstr{\"o}m and Maria Eldh and Celine Elie-Caille and Agustin Enciso-Martinez and Rezvan Esmaeili and Camille Ettelaie and F{\"o}rs{\"o}nits, {Andr{\'a}s I.} and Muller Fabbri and Marco Falasca and Hongkuan Fan and Farah Fatima and Alireza Fazeli and Mar{\'i}a Fern{\'a}ndez-Rhodes and Christopher Fernandez-Prada and Ferraro, {Mariola J.} and Ferreira, {Joao N.} and Ferreira, {Rafaela F.} and Figueroa-Hall, {Leandra K.} and Figueroa-Vald{\'e}s, {Aliosha I.} and Fioretti, {Paolo V.} and Sabine Flenady and Miguel Flores-Bellver and Fok, {Ellis K.} and Pamali Fonseka and Karen Forbes and Ford, {Verity J.} and Cristina Fornaguera and Dorian Forte and Stefano Forte and Orazio Fortunato and Franklin, {Jeffrey L.} and Daniela Freitas and Annie Frelet-Barrand and Yu Fujita and Kathrin G{\"a}rtner and Andr{\'e} G{\"o}rgens and Gabriel, {{\'A}urea M.} and Martina Gabrielli and Susanne Gabrielsson and Alicia Galinsoga and Andrea Galisova and Gamage, {Teena K.J.B.} and Yingtang Gao and Marta Garcia-Contreras and Garcia, {M. Madhy Garcia} and Garcia, {Maria No{\'e}} and Ernesto Gargiulo and Garibotti, {Hern{\'a}n Gonz{\'a}lez King} and {Mc Gee}, {Margaret M.} and Genard, {G{\'e}raldine C.} and Fabiana Geraci and Jamal Ghanam and Subhadip Ghatak and Mahlegha Ghavami and Ghebosu, {Raluca E.} and Gho, {Yong Song} and Sayam Ghosal and Georgios Giamas and Bernd Giebel and Caroline Gilbert and Mario Gimona and Henrique Gir{\~a}o and Ilaria Giusti and Gizzie, {Evan A.} and Sofija Glamo{\v c}lija and Glass, {Sarah E.} and Jessica Gobbo and Nihar Godbole and Goetz, {Jacky G.} and Olesia Gololobova and Manuel Gomez-Florit and Goncalves, {Jenifer Pendiuk} and Cansu Gorgun and Alessandro Gori and Sabina Gorska and Graner, {Michael W.} and Grau, {Georges E.} and Laura Grech and Greening, {David W.} and Gro{\ss}, {R{\"u}diger M.} and Gross, {Julia C.} and Jens Gruber and Alice Gualerzi and Dominic Guanzon and Gudbergsson, {Johann M.} and Guerin, {Coralie L.} and Flora Guerra and Guill{\'e}n, {Maria I.} and Vikramsingh Gujar and Wei Guo and Gupta, {Veer Bala} and Gupta, {Vivek Kumar} and Dakota Gustafson and Edina Gyukity-Sebesty{\'e}n and Patrick H{\"o}lker and Hade, {Mangesh D.} and Hagey, {Daniel W.} and Chungmin Han and Pingping Han and Rikinari Hanayama and Aase Handberg and Masako Harada and Maria Harmati and Paul Harrison and Harrison, {Rane A.} and Haynes, {Paul A.} and Mei He and Hargita Hegyesi and Hisey, {Colin L.} and Hochberg, {Fred H.} and {Nolte-{\textquoteright}t Hoen}, {Esther N.M.} and Marija Holcar and Beth Holder and Wolfgang Holnthoner and Harry Holthofer and Hooper, {D. Craig} and Elham Hosseini-Beheshti and Baharak Hosseinkhani and Jane Howard and Howe, {Kathryn L.} and Hoyle, {Nicholas R.} and Jiri Hrdy and Guoku Hu and Yiyao Huang and Veronica Huber and Samo Hudoklin and Antonia Hufnagel and Hulett, {Mark D.} and Stuart Hunt and Vincent Hyenne and {Di Ianni}, Emilio and Dalila Iannotta and Ibrahim, {Ahmed G.E.} and Ibrahim, {Sherif A.} and Seiko Ikezu and Tsuneya Ikezu and Hyungsoon Im and Inal, {Jameel M.} and Aleksandra Inic-Kanada and Marit Inngjerdingen and Yasuo Inoshima and Alena Ivanova and Elena Izquierdo and J{\o}rgensen, {Malene M{\o}ller} and Jackson, {Hannah K.} and Soren Jacobsen and Fernanda Jadue and Naureen Javeed and Jay, {Steven M.} and Muthuvel Jayachandran and Jayasinghe, {Migara K.} and Guido Jenster and Jeppesen, {Dennis K.} and Carmen Jer{\'o}nimo and Linglei Jiang and Jing Jin and Kentaro Jingushi and Jo, {Dong Gyu} and Joerger-Messerli, {Marianne S.} and Jones, {Jennifer C.} and Jones, {Melissa K.} and {de Jong}, {Olivier G.} and Ferrante, {Anthony W.} and Coleman, {Leon G.} and David Juncker and Stephanie Jung and Benjamin Jurek and Marcin Jurga and Verline Justilien and Mehdi Kabani and Raghu Kalluri and Masood Kamali-Moghaddam and Masamitsu Kanada and Taeyoung Kang and Kano, {Shin Ichi} and Maria Kaparakis-Liaskos and Elz˙Bieta Karnas and Antoine Karoichan and Fatah Kashanchi and Kashani, {Sara Assar} and Kashyap, {Namita N.} and Miroslava Katsur and Silvio Kau-Strebinger and Kauffman, {Amy C.} and Sukhbir Kaur and Oksana Kehoe and Kelwick, {Richard J.R.} and Kenari, {Amirmohammad Nasiri} and Kestecher, {Brachyahu M.} and Keulers, {Tom G.} and {Van Keuren-Jensen}, {Kendall R.} and Kasra Khalaj and Delaram Khamari and Ramin Khanabdali and Elena Khomyakova and Amanda Khoo and Kim, {Daniel H.} and Dongin Kim and Kim, {Han Sang} and Kim, {In San} and Soo Kim and Yohan Kim and Kima, {Peter E.} and Thomas Kislinger and Mikael Klingeborn and Rob Knight and Hiroaki Komuro and Anna Koncz and Timothea Konstantinou and {van der Koog}, Luke and Kooijmans, {Sander A.A.} and Kornek, {Miroslaw T.} and Maja Kosanovi{\'c} and Enis Kostallari and Koukoulis, {Tiana F.} and Stella Kourembanas and Kr{\"a}meralbers, {Eva Maria} and Veronika Kralj-Iglic and Susanne Krasemann and Krasnodembskaya, {Anna D.} and Krawczynska, {Natalia J.} and Kreft, {Mateja E.} and Kuehn, {Meta J.} and Kuipers, {Marije E.} and Konxhe Kulaj and Julia Kuligowski and Yumi Kumagai and Ashish Kumar and Saroj Kumar and Sharad Kumar and Meena Kumari and Gabrielis Kundrotas and Kurochkin, {Igor V.} and Masahiko Kuroda and Marzena Kurzawa-Akanbi and Kweskin, {Sasha J.} and Elisa L{\'a}zaro-Ib{\'a}{\~n}ez and {\'A}kosm L{\H o}rincz and Andrew Lai and Lai, {Charles P.} and Saara Laitinen and Solange Landreville and Sigrun Lange and Langevin, {Scott M.} and Langlois, {Marc Andr{\'e}} and Languino, {Lucia R.} and Joanne Lannigan and Lark, {Daniel S.} and Larregina, {Adriana T.} and Laurent, {Louise C.} and David Laurin and Gregory Lavieu and Charlotte Lawson and {Le Lay}, Soazig and Kevin Leandro and Aur{\'e}lie Ledreux and Changjin Lee and Lee, {Dong Sup} and Hakho Lee and Heonjin Lee and Lee, {Sun Young} and Lee, {Tae Ryong} and Lee, {Wai Leng} and Iliya Lefterov and Xinhua Lei and Janne Leivo and Quentin Lemaire and Leme, {Adriana F.Paes} and Lemon, {Stanley M.} and Stephen Lenzini and Jonathan Leor and Efrat Levy and Bo Li and Guoping Li and Li, {Jiao Jiao} and Qiubai Li and Xinlei Li and Xiuming Liang and Rebecca Lim and Tania Limongi and Aija Linē and Lins, {Paula Pincela} and Lien Lippens and Guanshu Liu and Alicia Llorente and Longjohn, {Modeline N.} and {van de Loo}, {Fons A.J.} and Lorenowicz, {Magdalena J.} and Aurelio Lorico and Olivier Loudig and Xavier Loyer and Estefan{\'i}a Lozano-Andr{\'e}s and Biao Lu and Quan Lu and Quentin Lubart and Fabrice Lucien and Lunavat, {Taral R.} and Lundberg, {Ludwig Ermann} and Lundy, {David J.} and Luoto, {Jens C.} and Lyden, {David C.} and M{\"u}ller, {Janis A.} and Macphee, {Daniel J.} and Elise Madec and Maga{\~n}a, {Setty M.} and Vasiliki Mahairaki and Harmeet Malhi and Malnou, {C{\'e}cile E.} and Mamand, {Doste R.} and Kenny Man and Mauro Manno and Mantel, {Pierre Yves} and Tecla Marangon and Eduardo Marb{\'a}n and Antonio Marcilla and Maremanda, {Krishna P.} and Leonid Margolis and Luis Mari{\~n}as-Pardo and Ivica Mari{\'c} and Mart{\'i}n, {Silvia S{\'a}nchez} and Eduardo Mart{\'i}nez-Mart{\'i}nez and Catherine Martel and Pilar Martin-Duque and Lorena Martin-Jaular and Martinez-Murillo, {Paola A.} and Sarai Martinez-Pacheco and Tania Martins-Marques and Benjamin Mary and Marzan, {Akbar L.} and Andreu Matamoros-Angles and Suresh Mathivanan and Juntaro Matsuzaki and Mayan, {Maria D.} and Carla Mazzeo and Mariama Mbengue and McCann, {Mark J.} and McIlvenna, {Luke C.} and McVey, {Mark J.} and Nicole Meisner-Kober and Maiken Mellergaard and Giorgia Melli and Kerstin Menck and Menjivar, {Nico G.} and Ramkumar Menon and Mentkowski, {Kyle I.} and Miklavcic, {John J.} and Miklosi, {Andras G.} and Bojana Milutinovic and Minciacchi, {Valentina R.} and Mehdi Mirzaei and Shalini Mishra and Mitchell, {Megan I.} and Danilo Mladenovi{\'c} and Eqbal Mohamadi and Fatemeh Momen-Heravi and Mondal, {Sujan K.} and Marta Mongui{\'o}-Tortajada and Jisook Moon and Morandi, {Mattia I.} and Violaine Moreau and Moreira, {Lissette Retana} and Morelli, {Adrian E.} and Mori, {Marcelo A.} and Masahiro Morimoto and Mathilde Mosser and Motaung, {Thabiso E.} and Etienne Moussay and Vera Mugoni and Francois Mullier and Maurizio Muraca and Saravanakumar Murugesan and Luca Musante and Angelo Music{\`o} and Afrodit{\'e} N{\'e}meth and Krisztina N{\'e}meth and Am{\'e}lie Nadeau and Nam, {Gi Hoon} and Honami Naora and Riccardo Natoli and Muhammad Nawaz and Irina Nazarenko and Ndukaife, {Justus C.} and Christina Nedeva and Peter Nejsum and Inge Nelissen and Christian Neri and Tommaso Neri and Paolo Neviani and Ng, {Chiew Yong} and {van Niel}, Guillaume and Nadezhda Nikiforova and Leonardo Nimrichter and Chitranshi Nitin and Njock, {Makon S{\'e}bastien} and Daniele No{\"e}l and Alessio Noghero and Nolan, {John P.} and Sam Noppen and Hooten, {Nicole Noren} and {da Silva Novaes}, Antonio and Ana O{\textquoteright}loghlen and Johannes Oesterreicher and Seungw Oh and Attila Ol{\'a}h and Martin Olivier and Ong, {Siew Ling} and Angelica Ortiz and Ortiz, {Luis A.} and Ole {\O}stergaard and Osorio, {Omar A.} and Xabier Osteikoetxea and Matias Ostrowski and David Otaegui and Alexander Otahal and Ozawa, {Patricia M.M.} and Ozkocak, {Dilara C.} and Krisztina P{\'a}l{\'o}czi and Roc{\'i}o P{\'e}rez-Gonz{\'a}lez and Pachane, {Bianca C.} and Hafiza Padinharayil and Daan Paget and Jerome Paggetti and Palacio, {Paola C.Loreto} and Pallasch, {Christian P.} and Roberta Palmulli and Bairen Pang and Liliia Paniushkina and Paschalia Pantazi and Lucia Paolini and Papademetrio, {Daniela L.} and Pietro Parisse and Park, {Dong Jun} and Juhee Park and Park, {Young Gyun} and Patton, {James G.} and Peake, {Nicholas J.} and Pegtel, {D. Michiel} and H{\'e}ctor Peinado and Francesca Perut and Pfaffl, {Michael W.} and Annika Pfeiffer and Phan, {Thanh Kha} and Phinney, {Donald G.} and Phylactou, {Leonidas A.} and Silvia Picciolini and Monika Pietrowska and Max Piffoux and Cl{\'a}udio Pinheiro and Pink, {Ryan C.} and Pleet, {Michelle L.} and Gabriella Pocsfalvi and Poh, {Qi Hui} and Ganesha Poojary and Poon, {Ivan K.H.} and Giuseppina Poppa and {Del Portillo}, {Hernando A.} and Vendula Pospichalova and Shirley Potter and Powell, {Bonita H.} and Powis, {Simon J.} and Ilaria Prada and Indira Prasadam and Christian Preu{\ss}er and Pua, {Heather H.} and Ferdinando Pucci and Florian Puhm and Berta Puig and Lynn Pulliam and Adityas Purnianto and Puutio, {Johanna M.M.} and Quilang, {Rachel C.} and Rabbani, {Piul S.} and Gorjana Rackov and Annalisa Radeghieri and Radu, {Claudia M.} and Raffai, {Robert L.} and Alok Raghav and Mohammad Rahbari and Rahman, {MD Matiur} and Rahman, {Md Mostafizur} and Rai, {Alex J.} and Stefania Raimondo and Sneha Raju and Janusz Rak and Lausonia Ramaswamy and Javier Ramirezricardo and Ramirez, {Marcel I.} and Shikha Rani and Graca Raposo and Rather, {Hilal A.} and Agnieszka Razim and Antonia Reale and Eduardo Reategui and Reddel, {Caroline J.} and Reddy, {Shivakumar K.} and Stephen Redenti and Reed, {Samantha L.} and Neta Regevrudzki and Reiners, {Katrin S.} and Nata{\v s}a Resnik and Rice, {Gregory E.} and Ricklefs, {Franz L.} and Kirsi Rilla and Rimmer, {Michael P.} and Roballo, {Kelly C.S.} and Robbins, {Paul D.} and Roberts, {David D.} and Jordi Roca and Rodal, {Avital A.} and Rodrigues-Junior, {Dorival M.} and Rodrigues, {Marcio L.} and Roefs, {Marieke T.} and Rogers, {Russell G.} and Rita Romani and Miriam Romano and Sophie Rome and Rok Romih and Anna Romolo and Rossi, {Tatiane De} and Rouschop, {Kasper M.} and Routenberg, {David A.} and Quentin Roux and {van Royen}, {Martin E.} and Rozo, {Anna{\"i}g J.} and David Rufino-Ramos and Aurelia Rughetti and Russell, {Ashley E.} and Rutter, {Stephanie F.} and Rysmakhanov, {Myltykbay S.} and S{\'a}nchez, {Catherine A.} and Yoel Sadovsky and Reihaneh Safavi-Sohi and Ram Sagar and Saidu, {Nathaniel E.B.} and Julien Saint-Pol and Edison Salas-Huenuleo and Salazar-Puerta, {Ana I.} and Ayesha Saleem and Salekdeh, {Ghasem Hosseini} and Carlos Salomon and Amanda Salviano-Silva and Salybekov, {Amankeldi A.} and Mark Samuels and Santavanond, {Jascinta P.} and Jessie Santoro and Mark Santos and Rahul Sanwlani and Saul, {Meike J.} and Sch{\o}yen, {Tine Hiorth} and Irma Schabussova and Emilia Scharrig and Randy Schekman and Jessica Schiavi-Tritz and Schiffelers, {Raymond M.} and Schmid, {Anna M.} and Raphael Schneider and Stefan Schneider and Andreina Schoeberlein and Schorey, {Jeffrey S.} and Naohiro Seo and Joaquin Seras-Franzoso and Sanjay Shahi and Olga Shatnyeva and Shea, {Deanna F.} and Shelke, {Ganesh V.} and Shetty, {Ashok K.} and Kiyotaka Shiba and Shiju, {Thomas Michael} and Surya Shrivastava and Sachin Shukla and Siljander, {Pia R.M.} and Silva, {Andreia M.} and Singh, {Ajay P.} and Sangeeta Singh and Mikhail Skliar and Johan Skog and Sluijter, {Joost P.G.} and Snyder, {Orman L.} and Carolina Soekmadji and Ahmed Somaida and Masaharu Somiya and Karolina Soroczy{\'n}ska and Javier Sotillo and Fernando Souza-Fonseca-Guimaraes and Sheila Spada and Spiers, {Harry V.M.} and Spitzberg, {Joshua D.} and Akhil Srivastava and Srivastava, {Amit K.} and Ewa St{\c e}pie{\'n} and Frederic St-Denis-Bissonnette and Stahl, {Philip D.} and Janine Stam and Oumaima Stambouli and Stanton, {Bruce A.} and Stassen, {Frank R.M.} and Oskar Staufer and Lo{\"i}c Steiner and Ganna Stepanova and Veronika Stoka and Willem Stoorvogel and {von Strandmann}, {Elke Pogge} and Dirk Strunk and Stylli, {Stanley S.} and Huaqi Su and Subbaya Subramanian and Bingdong Sui and Sonal Sukreet and Elias Sulaiman and Sung, {Bong Hwan} and Vijaya Sunkara and Zucai Suo and Per Svenningsen and Julian Swatler and Symonds, {Emma K.C.} and Viktoria Szeifert and Szigy{\'a}rt{\'o}, {Imola Cs} and Eszter T{\'o}th and Ta{\c s}lı, {Neslihan P.} and Hidetoshi Tahara and Takahashi, {Ryou U.} and Yoshinobu Takakura and Osamu Takikawa and Kaloyan Takov and Tang, {Vera A.} and Simona Taverna and Nadim Tawil and Loes Teeuwen and Sandra Tejedor and Tobias Tertel and Abhimanyu Thakur and Tara Thompson-Felix and Changhai Tian and Aleksei Tikhonov and Swasti Tiwari and Toh, {Wei Seong} and Tomes, {John J.} and Elisa Tonoli and Trinidad, {Camille V.} and Lucienne Tritten and Rucha Trivedi and Zach Troyer and Migmar Tsamchoe and Vera Tscherrig and Thupten Tsering and Kristyna Turkova and Tutanov, {Oleg S.} and Koji Ueda and Dinesh Upadhya and Fumihiko Urabe and Lorena Urbanelli and Ornella Urz{\`i} and Elena Vacchi and Pieter Vader and Riccardo Vago and Hadi Valadi and Sami Valkonen and Manuel Varas-Godoy and Vasconcelos, {M. Helena} and Vechetti, {Ivan J.} and Veiga, {Sara I.} and Vella, {Laura J.} and {\'E}milie Velot and Verweij, {Frederik J.} and Beate Vestad and Ludovic Vinay and Margarida Viola and Tam{\'a}s Visnovitz and Vreeland, {Wyatt N.} and Vukman, {Krisztina V.} and Wade, {Philippa K.} and {van de Wakker}, {Simonides I.} and Lucas Walther and Tong Wang and Xiaoqin Wang and Watson, {Dionysios C.} and Weaver, {Alissa M.} and Webber, {Jason P.} and Viktoria Weber and Luisa Weiss and Weiss, {Mark L.} and Ren{\'e} Weiss and Ralph Weissleder and Yi Wen and {de Wever}, Olivier and Wheelock, {Asa M.} and White, {Katherine E.} and Bradley Whitehead and Whiteside, {Theresa L.} and Joseph Whitley and Zolt{\'a}n Wiener",

note = "{\textcopyright} 2024 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.",

year = "2024",

month = feb,

day = "7",

doi = "10.1002/jev2.12404",

language = "English",

volume = "13",

journal = "Journal of Extracellular Vesicles",

issn = "2001-3078",

publisher = "Wiley",

number = "2",

}

TY - JOUR

T1 - Minimal information for studies of extracellular vesicles (MISEV2023)

T2 - From basic to advanced approaches

AU - MISEV Consortium

AU - Welsh, Joshua A.

AU - Goberdhan, Deborah C.I.

AU - O’Driscoll, Lorraine

AU - Buzas, Edit I.

AU - Blenkiron, Cherie

AU - Bussolati, Benedetta

AU - Cai, Houjian

AU - Di Vizio, Dolores

AU - Driedonks, Tom A.P.

AU - Erdbrügger, Uta

AU - Falcon-Perez, Juan M.

AU - Fu, Qing Ling

AU - Hill, Andrew F.

AU - Lenassi, Metka

AU - Lim, Sai Kiang

AU - M˜y, G. Mahoney

AU - Mohanty, Sujata

AU - Möller, Andreas

AU - Nieuwland, Rienk

AU - Ochiya, Takahiro

AU - Sahoo, Susmita

AU - Torrecilhas, Ana C.

AU - Zheng, Lei

AU - Zijlstra, Andries

AU - Abuelreich, Sarah

AU - Bagabas, Reem

AU - Bergese, Paolo

AU - Bridges, Esther M.

AU - Brucale, Marco

AU - Burger, Dylan

AU - Carney, Randy P.

AU - Cocucci, Emanuele

AU - Crescitelli, Rossella

AU - Hanser, Edveena

AU - Harris, Adrian L.

AU - Haughey, Norman J.

AU - Hendrix, An

AU - Ivanov, Alexander R.

AU - Jovanovic-Talisman, Tijana

AU - Kruh-Garcia, Nicole A.

AU - Faustino, Vroniqa Ku’ulei Lyn

AU - Kyburz, Diego

AU - Lässer, Cecilia

AU - Lennon, Kathleen M.

AU - Lötvall, Jan

AU - Maddox, Adam L.

AU - Martens-Uzunova, Elena S.

AU - Mizenko, Rachel R.

AU - Newman, Lauren A.

AU - Ridolfi, Andrea

AU - Rohde, Eva

AU - Rojalin, Tatu

AU - Rowland, Andrew

AU - Saftics, Andras

AU - Sandau, Ursula S.

AU - Saugstad, Julie A.

AU - Shekari, Faezeh

AU - Swift, Simon

AU - Ter-Ovanesyan, Dmitry

AU - Tosar, Juan P.

AU - Useckaite, Zivile

AU - Valle, Francesco

AU - Varga, Zoltan

AU - van der Pol, Edwin

AU - van Herwijnen, Martijn J.C.

AU - Wauben, Marca H.M.

AU - Wehman, Ann M.

AU - Williams, Sarah

AU - Zendrini, Andrea

AU - Zimmerman, Alan J.

AU - Thery, Clotilde

AU - Witwer, Kenneth W.

AU - Ahmad, Samar

AU - Ahmed, Dina A.K.

AU - Ahmed, Sarah H.

AU - Aikawa, Elena

AU - Akbar, Naveed

AU - Akiyoshi, Kazunari

AU - Al-Adra, David P.

AU - Al-Masawa, Maimonah E.

AU - Albanese, Manuel

AU - Alberro, Ainhoa

AU - Alcaraz, María José

AU - Alexander-Brett, Jen

AU - Alexander, Kimberley L.

AU - Ali, Nilufar

AU - Alibhai, Faisal J.

AU - Allelein, Susann

AU - Allenby, Mark C.

AU - Almeida, Fausto

AU - de Almeida, Luis Pereira

AU - Almousa, Sameh W.

AU - Altan-Bonnet, Nihal

AU - Altei, Wanessa F.

AU - Alvarez-Llamas, Gloria

AU - Alvarez, Cora L.

AU - An, Hyo Jung

AU - Anand, Krishnan

AU - Andaloussi, Samir E.L.

AU - Anderson, Johnathon D.

AU - Andriantsitohaina, Ramaroson

AU - Ansari, Khairul I.

AU - Anselmo, Achille

AU - Antoniou, Anna

AU - Aqil, Farrukh

AU - Arab, Tanina

AU - Archer, Fabienne

AU - Arif, Syrine

AU - Armstrong, David A.

AU - Arntz, Onno J.

AU - Arsène, Pierre

AU - Arteaga-Blanco, Luis

AU - Asokan, Nandini

AU - Aspelin, Trude

AU - Atkin-Smith, Georgia K.

AU - Aubert, Dimitri

AU - Ayyar, Kanchana K.

AU - Azlan, Maryam

AU - Azoidis, Ioannis

AU - Bécot, Anaïs

AU - Bach, Jean Marie

AU - Bachurski, Daniel

AU - Bae, Seoyoon

AU - Bagge, Roger Olofsson

AU - Baj-Krzyworzeka, Monika

AU - Balaj, Leonora

AU - Balbi, Carolina

AU - van Balkom, Bas W.M.

AU - Ballal, Abhijna R.

AU - Bano, Afsareen

AU - Banzet, Sébastien

AU - Bare, Yonis

AU - Barile, Lucio

AU - Barman, Bahnisikha

AU - Barranco, Isabel

AU - Barreca, Valeria

AU - Bart, Geneviève

AU - Barteneva, Natasha S.

AU - Basso, Manuela

AU - Batish, Mona

AU - Bauer, Natalie R.

AU - Baxter, Amy A.

AU - Bazié, Wilfried W.

AU - Bazzan, Erica

AU - Beaumont, Joel E.J.

AU - Bebawy, Mary

AU - Bebelman, Maarten P.

AU - Bedina-Zavec, Apolonija

AU - Beetler, Danielle J.

AU - Beke-Somfai, Tamás

AU - Belleannée, Clémence

AU - Benedikter, Birke J.

AU - Benediktsdóttir, Berglind E.

AU - Berardi, Anna C.

AU - Bergamelli, Mathilde

AU - Bertolini, Irene

AU - Bhattacharyya, Asima

AU - Bhattacharyya, Suvendra N.

AU - Biller, Steven J.

AU - Billottet, Clotilde

AU - Bissler, John J.

AU - Blanc-Brude, Olivier

AU - Blijdorp, Charles J.

AU - Bobis-Wozowicz, Sylwia

AU - Bodart-Santos, Victor

AU - Bodnár, Bernadett R.

AU - Boilard, Eric

AU - Boireau, Wilfrid

AU - Bokun, Vladimir

AU - Bollard, Stephanie M.

AU - Bollini, Sveva

AU - Bongiovanni, Antonella

AU - Bongiovanni, Laura

AU - Bonifay, Amandine

AU - Boppart, Marni D.

AU - Borràs, Francesce

AU - Bosch, Steffi

AU - Boselli, Daniela

AU - Bottini, Massimo

AU - Bouffard, Jeff

AU - Boulanger, Chantal M.

AU - Boutros, Paul C.

AU - Boyadjian, Oscar

AU - Boysen, Anders T.

AU - Bozkurt, Batuhan T.

AU - Bramich, Kyle P.

AU - Braun, Fabian

AU - Bravo-Miana, Rocío Del Carmen

AU - Breakefield, Xandra O.

AU - Brenna, Santra

AU - Brennan, Kieran

AU - Brennan, Meadhbh

AU - Breyne, Koen

AU - Brigstock, David R.

AU - Brisson, Alain R.

AU - Brodie, Chaya

AU - Bruno, Katelyna

AU - Bucci, Cecilia

AU - Buch, Shilpa

AU - Buck, Amyh

AU - Bukva, Mátyás

AU - Bulte, Jeff W.M.

AU - Buratta, Sandra

AU - Burgy, Olivier

AU - Burnier, Julia V.

AU - Burrows, Kaiping

AU - Busatto, Sara

AU - Buzas, Krisztina

AU - Byrd, J. Brian

AU - Cáceres-Verschae, Albano

AU - Caires, Hugo R.

AU - Campos-Silva, Carmen

AU - Camussi, Giovanni

AU - de Candia, Paola

AU - Carceller, Carmen

AU - Fernandez-Becerra, Carmen

AU - Carrasco, Alexis G.Murillo

AU - Carter, David R.F.

AU - Cavallaro, Sara

AU - Cavallero, Serena

AU - Cavallero, Sophie

AU - Cerda-Troncoso, Cristóbal

AU - Chahwan, Richard

AU - Chalupská, Renata

AU - Chamley, Lawrence W.

AU - Chandra, Partha K.

AU - Chang, Wen Wei

AU - Charest, Al

AU - Chen, Chihchen

AU - Chen, Hao

AU - Chen, Qiang

AU - Chen, Shuai

AU - Chen, Siyu

AU - Chen, Yunxi

AU - Cheng, Lesley

AU - Chernyshev, Vasiliy S.

AU - Chetty, Venkatesh Kumar

AU - Chitti, Sai V.

AU - Cho, Ssang Goo

AU - Cho, Yoon Kyoung

AU - Choi, Byeong Hyeon

AU - Chutipongtanate, Somchai

AU - Cicardi, Maria Elena

AU - Cifuentes-Rius, Anna

AU - Ciullo, Alessandra

AU - Clayton, Aled

AU - Cleary, Jacob A.

AU - Cocozza, Federico

AU - Coffey, Robert J.

AU - Collino, Federica

AU - Colombo, Federico

AU - Colosetti, Pascal

AU - Compañ-Bertomeu, Alvaro

AU - Constanzo, Julie

AU - Corbeil, Denis

AU - Cordeiro-Da-Silva, Anabela

AU - Costa, Júlia

AU - Couch, Yvonne

AU - Courageux, Yvan

AU - Coutant, Kelly

AU - Coyle, Beth

AU - Cretich, Marina

AU - Cronemberger-Andrade, André

AU - Crossland, Rachel E.

AU - Cucher, Marcela A.

AU - Czystowska-Kuzmicz, Malgorzata

AU - D’Acunzo, Pasquale

AU - D’Agnano, Igea

AU - D’agostino, Vito G.

AU - D’Arrigo, Daniele

AU - D’Souza-Schorey, Crislyn

AU - Dagur, Raghubendra S.

AU - Danielson, Kirsty M.

AU - Das, Saumya

AU - Dauphin, Thibaud

AU - Davidson, Sean M.

AU - Davies, Owen G.

AU - Davies, Rebecca L.

AU - Davis, Chelsea N.

AU - Deep, Gagan

AU - Degosserie, Jonathan

AU - Van Delen, Mats

AU - Deliwala, Vatsal

AU - Dellar, Elizabeth R.

AU - Van Deun, Jan

AU - Dev, Apurba

AU - Deville, Sarah

AU - Devitt, Andrew

AU - Dhondt, Bert

AU - Dieterich, Lothar C.

AU - Dittmer, Dirk P.

AU - Dobosh, Brian

AU - Dobra, Gabriella

AU - Dogra, Navneet

AU - Dohi, Eisuke

AU - Dolo, Vincenza

AU - Domashevich, Timothy V.

AU - Dominici, Massimo

AU - Dong, Liang

AU - Doré, Etienne

AU - Dragovic, Rebecca A.

AU - Drittanti, Lila

AU - Droste, Marvin

AU - Duan, Wei

AU - Durmaz, Esmahan

AU - Dutta, Suman

AU - Eguchi, Takanori

AU - Eichenberger, Ramon M.

AU - Eitan, Erez

AU - Ekström, Karin

AU - Eldh, Maria

AU - Elie-Caille, Celine

AU - Enciso-Martinez, Agustin

AU - Esmaeili, Rezvan

AU - Ettelaie, Camille

AU - Försönits, András I.

AU - Fabbri, Muller

AU - Falasca, Marco

AU - Fan, Hongkuan

AU - Fatima, Farah

AU - Fazeli, Alireza

AU - Fernández-Rhodes, María

AU - Fernandez-Prada, Christopher

AU - Ferraro, Mariola J.

AU - Ferreira, Joao N.

AU - Ferreira, Rafaela F.

AU - Figueroa-Hall, Leandra K.

AU - Figueroa-Valdés, Aliosha I.

AU - Fioretti, Paolo V.

AU - Flenady, Sabine

AU - Flores-Bellver, Miguel

AU - Fok, Ellis K.

AU - Fonseka, Pamali

AU - Forbes, Karen

AU - Ford, Verity J.

AU - Fornaguera, Cristina

AU - Forte, Dorian

AU - Forte, Stefano

AU - Fortunato, Orazio

AU - Franklin, Jeffrey L.

AU - Freitas, Daniela

AU - Frelet-Barrand, Annie

AU - Fujita, Yu

AU - Gärtner, Kathrin

AU - Görgens, André

AU - Gabriel, Áurea M.

AU - Gabrielli, Martina

AU - Gabrielsson, Susanne

AU - Galinsoga, Alicia

AU - Galisova, Andrea

AU - Gamage, Teena K.J.B.

AU - Gao, Yingtang

AU - Garcia-Contreras, Marta

AU - Garcia, M. Madhy Garcia

AU - Garcia, Maria Noé

AU - Gargiulo, Ernesto

AU - Garibotti, Hernán González King

AU - Mc Gee, Margaret M.

AU - Genard, Géraldine C.

AU - Geraci, Fabiana

AU - Ghanam, Jamal

AU - Ghatak, Subhadip

AU - Ghavami, Mahlegha

AU - Ghebosu, Raluca E.

AU - Gho, Yong Song

AU - Ghosal, Sayam

AU - Giamas, Georgios

AU - Giebel, Bernd

AU - Gilbert, Caroline

AU - Gimona, Mario

AU - Girão, Henrique

AU - Giusti, Ilaria

AU - Gizzie, Evan A.

AU - Glamočlija, Sofija

AU - Glass, Sarah E.

AU - Gobbo, Jessica

AU - Godbole, Nihar

AU - Goetz, Jacky G.

AU - Gololobova, Olesia

AU - Gomez-Florit, Manuel

AU - Goncalves, Jenifer Pendiuk

AU - Gorgun, Cansu

AU - Gori, Alessandro

AU - Gorska, Sabina

AU - Graner, Michael W.

AU - Grau, Georges E.

AU - Grech, Laura

AU - Greening, David W.

AU - Groß, Rüdiger M.

AU - Gross, Julia C.

AU - Gruber, Jens

AU - Gualerzi, Alice

AU - Guanzon, Dominic

AU - Gudbergsson, Johann M.

AU - Guerin, Coralie L.

AU - Guerra, Flora

AU - Guillén, Maria I.

AU - Gujar, Vikramsingh

AU - Guo, Wei

AU - Gupta, Veer Bala

AU - Gupta, Vivek Kumar

AU - Gustafson, Dakota

AU - Gyukity-Sebestyén, Edina

AU - Hölker, Patrick

AU - Hade, Mangesh D.

AU - Hagey, Daniel W.

AU - Han, Chungmin

AU - Han, Pingping

AU - Hanayama, Rikinari

AU - Handberg, Aase

AU - Harada, Masako

AU - Harmati, Maria

AU - Harrison, Paul

AU - Harrison, Rane A.

AU - Haynes, Paul A.

AU - He, Mei

AU - Hegyesi, Hargita

AU - Hisey, Colin L.

AU - Hochberg, Fred H.

AU - Nolte-’t Hoen, Esther N.M.

AU - Holcar, Marija

AU - Holder, Beth

AU - Holnthoner, Wolfgang

AU - Holthofer, Harry

AU - Hooper, D. Craig

AU - Hosseini-Beheshti, Elham

AU - Hosseinkhani, Baharak

AU - Howard, Jane

AU - Howe, Kathryn L.

AU - Hoyle, Nicholas R.

AU - Hrdy, Jiri

AU - Hu, Guoku

AU - Huang, Yiyao

AU - Huber, Veronica

AU - Hudoklin, Samo

AU - Hufnagel, Antonia

AU - Hulett, Mark D.

AU - Hunt, Stuart

AU - Hyenne, Vincent

AU - Di Ianni, Emilio

AU - Iannotta, Dalila

AU - Ibrahim, Ahmed G.E.

AU - Ibrahim, Sherif A.

AU - Ikezu, Seiko

AU - Ikezu, Tsuneya

AU - Im, Hyungsoon

AU - Inal, Jameel M.

AU - Inic-Kanada, Aleksandra

AU - Inngjerdingen, Marit

AU - Inoshima, Yasuo

AU - Ivanova, Alena

AU - Izquierdo, Elena

AU - Jørgensen, Malene Møller

AU - Jackson, Hannah K.

AU - Jacobsen, Soren

AU - Jadue, Fernanda

AU - Javeed, Naureen

AU - Jay, Steven M.

AU - Jayachandran, Muthuvel

AU - Jayasinghe, Migara K.

AU - Jenster, Guido

AU - Jeppesen, Dennis K.

AU - Jerónimo, Carmen

AU - Jiang, Linglei

AU - Jin, Jing

AU - Jingushi, Kentaro

AU - Jo, Dong Gyu

AU - Joerger-Messerli, Marianne S.

AU - Jones, Jennifer C.

AU - Jones, Melissa K.

AU - de Jong, Olivier G.

AU - Ferrante, Anthony W.

AU - Coleman, Leon G.

AU - Juncker, David

AU - Jung, Stephanie

AU - Jurek, Benjamin

AU - Jurga, Marcin

AU - Justilien, Verline

AU - Kabani, Mehdi

AU - Kalluri, Raghu

AU - Kamali-Moghaddam, Masood

AU - Kanada, Masamitsu

AU - Kang, Taeyoung

AU - Kano, Shin Ichi

AU - Kaparakis-Liaskos, Maria

AU - Karnas, Elz˙Bieta

AU - Karoichan, Antoine

AU - Kashanchi, Fatah

AU - Kashani, Sara Assar

AU - Kashyap, Namita N.

AU - Katsur, Miroslava

AU - Kau-Strebinger, Silvio

AU - Kauffman, Amy C.

AU - Kaur, Sukhbir

AU - Kehoe, Oksana

AU - Kelwick, Richard J.R.

AU - Kenari, Amirmohammad Nasiri

AU - Kestecher, Brachyahu M.

AU - Keulers, Tom G.

AU - Van Keuren-Jensen, Kendall R.

AU - Khalaj, Kasra

AU - Khamari, Delaram

AU - Khanabdali, Ramin

AU - Khomyakova, Elena

AU - Khoo, Amanda

AU - Kim, Daniel H.

AU - Kim, Dongin

AU - Kim, Han Sang

AU - Kim, In San

AU - Kim, Soo

AU - Kim, Yohan

AU - Kima, Peter E.

AU - Kislinger, Thomas

AU - Klingeborn, Mikael

AU - Knight, Rob

AU - Komuro, Hiroaki

AU - Koncz, Anna

AU - Konstantinou, Timothea

AU - van der Koog, Luke

AU - Kooijmans, Sander A.A.

AU - Kornek, Miroslaw T.

AU - Kosanović, Maja

AU - Kostallari, Enis

AU - Koukoulis, Tiana F.

AU - Kourembanas, Stella

AU - Krämeralbers, Eva Maria

AU - Kralj-Iglic, Veronika

AU - Krasemann, Susanne

AU - Krasnodembskaya, Anna D.

AU - Krawczynska, Natalia J.

AU - Kreft, Mateja E.

AU - Kuehn, Meta J.

AU - Kuipers, Marije E.

AU - Kulaj, Konxhe

AU - Kuligowski, Julia

AU - Kumagai, Yumi

AU - Kumar, Ashish

AU - Kumar, Saroj

AU - Kumar, Sharad

AU - Kumari, Meena

AU - Kundrotas, Gabrielis

AU - Kurochkin, Igor V.

AU - Kuroda, Masahiko

AU - Kurzawa-Akanbi, Marzena

AU - Kweskin, Sasha J.

AU - Lázaro-Ibáñez, Elisa

AU - Lőrincz, Ákosm

AU - Lai, Andrew

AU - Lai, Charles P.

AU - Laitinen, Saara

AU - Landreville, Solange

AU - Lange, Sigrun

AU - Langevin, Scott M.

AU - Langlois, Marc André

AU - Languino, Lucia R.

AU - Lannigan, Joanne

AU - Lark, Daniel S.

AU - Larregina, Adriana T.

AU - Laurent, Louise C.

AU - Laurin, David

AU - Lavieu, Gregory

AU - Lawson, Charlotte

AU - Le Lay, Soazig

AU - Leandro, Kevin

AU - Ledreux, Aurélie

AU - Lee, Changjin

AU - Lee, Dong Sup

AU - Lee, Hakho

AU - Lee, Heonjin

AU - Lee, Sun Young

AU - Lee, Tae Ryong

AU - Lee, Wai Leng

AU - Lefterov, Iliya

AU - Lei, Xinhua

AU - Leivo, Janne

AU - Lemaire, Quentin

AU - Leme, Adriana F.Paes

AU - Lemon, Stanley M.

AU - Lenzini, Stephen

AU - Leor, Jonathan

AU - Levy, Efrat

AU - Li, Bo

AU - Li, Guoping

AU - Li, Jiao Jiao

AU - Li, Qiubai

AU - Li, Xinlei

AU - Liang, Xiuming

AU - Lim, Rebecca

AU - Limongi, Tania

AU - Linē, Aija

AU - Lins, Paula Pincela

AU - Lippens, Lien

AU - Liu, Guanshu

AU - Llorente, Alicia

AU - Longjohn, Modeline N.

AU - van de Loo, Fons A.J.

AU - Lorenowicz, Magdalena J.

AU - Lorico, Aurelio

AU - Loudig, Olivier

AU - Loyer, Xavier

AU - Lozano-Andrés, Estefanía

AU - Lu, Biao

AU - Lu, Quan

AU - Lubart, Quentin

AU - Lucien, Fabrice

AU - Lunavat, Taral R.

AU - Lundberg, Ludwig Ermann

AU - Lundy, David J.

AU - Luoto, Jens C.

AU - Lyden, David C.

AU - Müller, Janis A.

AU - Macphee, Daniel J.

AU - Madec, Elise

AU - Magaña, Setty M.

AU - Mahairaki, Vasiliki

AU - Malhi, Harmeet

AU - Malnou, Cécile E.

AU - Mamand, Doste R.

AU - Man, Kenny

AU - Manno, Mauro

AU - Mantel, Pierre Yves

AU - Marangon, Tecla

AU - Marbán, Eduardo

AU - Marcilla, Antonio

AU - Maremanda, Krishna P.

AU - Margolis, Leonid

AU - Mariñas-Pardo, Luis

AU - Marić, Ivica

AU - Martín, Silvia Sánchez

AU - Martínez-Martínez, Eduardo

AU - Martel, Catherine

AU - Martin-Duque, Pilar

AU - Martin-Jaular, Lorena

AU - Martinez-Murillo, Paola A.

AU - Martinez-Pacheco, Sarai

AU - Martins-Marques, Tania

AU - Mary, Benjamin

AU - Marzan, Akbar L.

AU - Matamoros-Angles, Andreu

AU - Mathivanan, Suresh

AU - Matsuzaki, Juntaro

AU - Mayan, Maria D.

AU - Mazzeo, Carla

AU - Mbengue, Mariama

AU - McCann, Mark J.

AU - McIlvenna, Luke C.

AU - McVey, Mark J.

AU - Meisner-Kober, Nicole

AU - Mellergaard, Maiken

AU - Melli, Giorgia

AU - Menck, Kerstin

AU - Menjivar, Nico G.

AU - Menon, Ramkumar

AU - Mentkowski, Kyle I.

AU - Miklavcic, John J.

AU - Miklosi, Andras G.

AU - Milutinovic, Bojana

AU - Minciacchi, Valentina R.

AU - Mirzaei, Mehdi

AU - Mishra, Shalini

AU - Mitchell, Megan I.

AU - Mladenović, Danilo

AU - Mohamadi, Eqbal

AU - Momen-Heravi, Fatemeh

AU - Mondal, Sujan K.

AU - Monguió-Tortajada, Marta

AU - Moon, Jisook

AU - Morandi, Mattia I.

AU - Moreau, Violaine

AU - Moreira, Lissette Retana

AU - Morelli, Adrian E.

AU - Mori, Marcelo A.

AU - Morimoto, Masahiro

AU - Mosser, Mathilde

AU - Motaung, Thabiso E.

AU - Moussay, Etienne

AU - Mugoni, Vera

AU - Mullier, Francois

AU - Muraca, Maurizio

AU - Murugesan, Saravanakumar

AU - Musante, Luca

AU - Musicò, Angelo

AU - Németh, Afrodité

AU - Németh, Krisztina

AU - Nadeau, Amélie

AU - Nam, Gi Hoon

AU - Naora, Honami

AU - Natoli, Riccardo

AU - Nawaz, Muhammad

AU - Nazarenko, Irina

AU - Ndukaife, Justus C.

AU - Nedeva, Christina

AU - Nejsum, Peter

AU - Nelissen, Inge

AU - Neri, Christian

AU - Neri, Tommaso

AU - Neviani, Paolo

AU - Ng, Chiew Yong

AU - van Niel, Guillaume

AU - Nikiforova, Nadezhda

AU - Nimrichter, Leonardo

AU - Nitin, Chitranshi

AU - Njock, Makon Sébastien

AU - Noël, Daniele

AU - Noghero, Alessio

AU - Nolan, John P.

AU - Noppen, Sam

AU - Hooten, Nicole Noren

AU - da Silva Novaes, Antonio

AU - O’loghlen, Ana

AU - Oesterreicher, Johannes

AU - Oh, Seungw

AU - Oláh, Attila

AU - Olivier, Martin

AU - Ong, Siew Ling

AU - Ortiz, Angelica

AU - Ortiz, Luis A.

AU - Østergaard, Ole

AU - Osorio, Omar A.

AU - Osteikoetxea, Xabier

AU - Ostrowski, Matias

AU - Otaegui, David

AU - Otahal, Alexander

AU - Ozawa, Patricia M.M.

AU - Ozkocak, Dilara C.

AU - Pálóczi, Krisztina

AU - Pérez-González, Rocío

AU - Pachane, Bianca C.

AU - Padinharayil, Hafiza

AU - Paget, Daan

AU - Paggetti, Jerome

AU - Palacio, Paola C.Loreto

AU - Pallasch, Christian P.

AU - Palmulli, Roberta

AU - Pang, Bairen

AU - Paniushkina, Liliia

AU - Pantazi, Paschalia

AU - Paolini, Lucia

AU - Papademetrio, Daniela L.

AU - Parisse, Pietro

AU - Park, Dong Jun

AU - Park, Juhee

AU - Park, Young Gyun

AU - Patton, James G.

AU - Peake, Nicholas J.

AU - Pegtel, D. Michiel

AU - Peinado, Héctor

AU - Perut, Francesca

AU - Pfaffl, Michael W.

AU - Pfeiffer, Annika

AU - Phan, Thanh Kha

AU - Phinney, Donald G.

AU - Phylactou, Leonidas A.

AU - Picciolini, Silvia

AU - Pietrowska, Monika

AU - Piffoux, Max

AU - Pinheiro, Cláudio

AU - Pink, Ryan C.

AU - Pleet, Michelle L.

AU - Pocsfalvi, Gabriella

AU - Poh, Qi Hui

AU - Poojary, Ganesha

AU - Poon, Ivan K.H.

AU - Poppa, Giuseppina

AU - Del Portillo, Hernando A.

AU - Pospichalova, Vendula

AU - Potter, Shirley

AU - Powell, Bonita H.

AU - Powis, Simon J.

AU - Prada, Ilaria

AU - Prasadam, Indira

AU - Preußer, Christian

AU - Pua, Heather H.

AU - Pucci, Ferdinando

AU - Puhm, Florian

AU - Puig, Berta

AU - Pulliam, Lynn

AU - Purnianto, Adityas

AU - Puutio, Johanna M.M.

AU - Quilang, Rachel C.

AU - Rabbani, Piul S.

AU - Rackov, Gorjana

AU - Radeghieri, Annalisa

AU - Radu, Claudia M.

AU - Raffai, Robert L.

AU - Raghav, Alok

AU - Rahbari, Mohammad

AU - Rahman, MD Matiur

AU - Rahman, Md Mostafizur

AU - Rai, Alex J.

AU - Raimondo, Stefania

AU - Raju, Sneha

AU - Rak, Janusz

AU - Ramaswamy, Lausonia

AU - Ramirezricardo, Javier

AU - Ramirez, Marcel I.

AU - Rani, Shikha

AU - Raposo, Graca

AU - Rather, Hilal A.

AU - Razim, Agnieszka

AU - Reale, Antonia

AU - Reategui, Eduardo

AU - Reddel, Caroline J.

AU - Reddy, Shivakumar K.

AU - Redenti, Stephen

AU - Reed, Samantha L.

AU - Regevrudzki, Neta

AU - Reiners, Katrin S.

AU - Resnik, Nataša

AU - Rice, Gregory E.

AU - Ricklefs, Franz L.

AU - Rilla, Kirsi

AU - Rimmer, Michael P.

AU - Roballo, Kelly C.S.

AU - Robbins, Paul D.

AU - Roberts, David D.

AU - Roca, Jordi

AU - Rodal, Avital A.

AU - Rodrigues-Junior, Dorival M.

AU - Rodrigues, Marcio L.

AU - Roefs, Marieke T.

AU - Rogers, Russell G.

AU - Romani, Rita

AU - Romano, Miriam

AU - Rome, Sophie

AU - Romih, Rok

AU - Romolo, Anna

AU - Rossi, Tatiane De

AU - Rouschop, Kasper M.

AU - Routenberg, David A.

AU - Roux, Quentin

AU - van Royen, Martin E.

AU - Rozo, Annaïg J.

AU - Rufino-Ramos, David

AU - Rughetti, Aurelia

AU - Russell, Ashley E.

AU - Rutter, Stephanie F.

AU - Rysmakhanov, Myltykbay S.

AU - Sánchez, Catherine A.

AU - Sadovsky, Yoel

AU - Safavi-Sohi, Reihaneh

AU - Sagar, Ram

AU - Saidu, Nathaniel E.B.

AU - Saint-Pol, Julien

AU - Salas-Huenuleo, Edison

AU - Salazar-Puerta, Ana I.

AU - Saleem, Ayesha

AU - Salekdeh, Ghasem Hosseini

AU - Salomon, Carlos

AU - Salviano-Silva, Amanda

AU - Salybekov, Amankeldi A.

AU - Samuels, Mark

AU - Santavanond, Jascinta P.

AU - Santoro, Jessie

AU - Santos, Mark

AU - Sanwlani, Rahul

AU - Saul, Meike J.

AU - Schøyen, Tine Hiorth

AU - Schabussova, Irma

AU - Scharrig, Emilia

AU - Schekman, Randy

AU - Schiavi-Tritz, Jessica

AU - Schiffelers, Raymond M.

AU - Schmid, Anna M.

AU - Schneider, Raphael

AU - Schneider, Stefan

AU - Schoeberlein, Andreina

AU - Schorey, Jeffrey S.

AU - Seo, Naohiro

AU - Seras-Franzoso, Joaquin

AU - Shahi, Sanjay

AU - Shatnyeva, Olga

AU - Shea, Deanna F.

AU - Shelke, Ganesh V.

AU - Shetty, Ashok K.

AU - Shiba, Kiyotaka

AU - Shiju, Thomas Michael

AU - Shrivastava, Surya

AU - Shukla, Sachin

AU - Siljander, Pia R.M.

AU - Silva, Andreia M.

AU - Singh, Ajay P.

AU - Singh, Sangeeta

AU - Skliar, Mikhail

AU - Skog, Johan

AU - Sluijter, Joost P.G.

AU - Snyder, Orman L.

AU - Soekmadji, Carolina

AU - Somaida, Ahmed

AU - Somiya, Masaharu

AU - Soroczyńska, Karolina

AU - Sotillo, Javier

AU - Souza-Fonseca-Guimaraes, Fernando

AU - Spada, Sheila

AU - Spiers, Harry V.M.

AU - Spitzberg, Joshua D.

AU - Srivastava, Akhil

AU - Srivastava, Amit K.

AU - Stępień, Ewa

AU - St-Denis-Bissonnette, Frederic

AU - Stahl, Philip D.

AU - Stam, Janine

AU - Stambouli, Oumaima

AU - Stanton, Bruce A.

AU - Stassen, Frank R.M.

AU - Staufer, Oskar

AU - Steiner, Loïc

AU - Stepanova, Ganna

AU - Stoka, Veronika

AU - Stoorvogel, Willem

AU - von Strandmann, Elke Pogge

AU - Strunk, Dirk

AU - Stylli, Stanley S.

AU - Su, Huaqi

AU - Subramanian, Subbaya

AU - Sui, Bingdong

AU - Sukreet, Sonal

AU - Sulaiman, Elias

AU - Sung, Bong Hwan

AU - Sunkara, Vijaya

AU - Suo, Zucai

AU - Svenningsen, Per

AU - Swatler, Julian

AU - Symonds, Emma K.C.

AU - Szeifert, Viktoria

AU - Szigyártó, Imola Cs

AU - Tóth, Eszter

AU - Taşlı, Neslihan P.

AU - Tahara, Hidetoshi

AU - Takahashi, Ryou U.

AU - Takakura, Yoshinobu

AU - Takikawa, Osamu

AU - Takov, Kaloyan

AU - Tang, Vera A.

AU - Taverna, Simona

AU - Tawil, Nadim

AU - Teeuwen, Loes

AU - Tejedor, Sandra

AU - Tertel, Tobias

AU - Thakur, Abhimanyu

AU - Thompson-Felix, Tara

AU - Tian, Changhai

AU - Tikhonov, Aleksei

AU - Tiwari, Swasti

AU - Toh, Wei Seong

AU - Tomes, John J.

AU - Tonoli, Elisa

AU - Trinidad, Camille V.

AU - Tritten, Lucienne

AU - Trivedi, Rucha

AU - Troyer, Zach

AU - Tsamchoe, Migmar

AU - Tscherrig, Vera

AU - Tsering, Thupten

AU - Turkova, Kristyna

AU - Tutanov, Oleg S.

AU - Ueda, Koji

AU - Upadhya, Dinesh

AU - Urabe, Fumihiko

AU - Urbanelli, Lorena

AU - Urzì, Ornella

AU - Vacchi, Elena

AU - Vader, Pieter

AU - Vago, Riccardo

AU - Valadi, Hadi

AU - Valkonen, Sami

AU - Varas-Godoy, Manuel

AU - Vasconcelos, M. Helena

AU - Vechetti, Ivan J.

AU - Veiga, Sara I.

AU - Vella, Laura J.

AU - Velot, Émilie

AU - Verweij, Frederik J.

AU - Vestad, Beate

AU - Vinay, Ludovic

AU - Viola, Margarida

AU - Visnovitz, Tamás

AU - Vreeland, Wyatt N.

AU - Vukman, Krisztina V.

AU - Wade, Philippa K.

AU - van de Wakker, Simonides I.

AU - Walther, Lucas

AU - Wang, Tong

AU - Wang, Xiaoqin

AU - Watson, Dionysios C.

AU - Weaver, Alissa M.

AU - Webber, Jason P.

AU - Weber, Viktoria

AU - Weiss, Luisa

AU - Weiss, Mark L.

AU - Weiss, René

AU - Weissleder, Ralph

AU - Wen, Yi

AU - de Wever, Olivier

AU - Wheelock, Asa M.

AU - White, Katherine E.

AU - Whitehead, Bradley

AU - Whiteside, Theresa L.

AU - Whitley, Joseph

AU - Wiener, Zoltán

PY - 2024/2/7

Y1 - 2024/2/7

N2 - Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

AB - Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

KW - ectosomes

KW - exosomes

KW - extracellular particles

KW - extracellular vesicles

KW - guidelines

KW - microparticles

KW - microvesicles

KW - minimal information requirements

KW - MISEV

KW - reproducibility

KW - rigor

KW - standardisation

KW - Exosomes/metabolism

KW - Biomarkers/metabolism

KW - Extracellular Vesicles/metabolism

KW - Phenotype

KW - Biological Transport

UR - http://www.scopus.com/inward/record.url?scp=85183359756&partnerID=8YFLogxK

U2 - 10.1002/jev2.12404

DO - 10.1002/jev2.12404

M3 - Article

C2 - 38326288

AN - SCOPUS:85183359756

SN - 2001-3078

VL - 13

JO - Journal of Extracellular Vesicles

JF - Journal of Extracellular Vesicles

IS - 2

M1 - e12404

ER -

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Abstract

Keywords

Access to Document

Permanent link

Other files and links

Fingerprint

Cite this